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Cortical Colonization by Plasmodiophora brassicae in Susceptible and Resistant Cabbage Cultivars

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Abstract

Three cultivars of cabbage (Brassica oleracea L. var. capitata) that differed in reaction to clubroot caused by Plasmodiophora brassicae were identified in field trials. Cultivar Bronco was susceptible to pathotype 6 (all plants severely clubbed), ‘Kilaherb’ was resistant (no plants with symptoms), and ‘B-2819’ was intermediate (small clubs). The development of P. brassicae in the roots of these cultivars was then assessed under controlled conditions at 28 days after inoculation using root sectioning, staining with methylene blue, and bright field microscopy. Plasmodia of P. brassicae were present in the root tissue of each cultivar. There was extensive cortical colonization (26 %) and some resting spore formation in ‘Bronco’, and lower colonization (9 %) and only trace levels of resting spore formation in ‘B-2819’. In the clubroot-resistant ‘Kilaherb’, root colonization was similar (7 %) to the intermediate ‘B-2819’, but no resting spores formed and no clubbing symptoms developed. This pattern of response differed substantially from that observed previously in commercial cultivars of spring canola (B. napus), and may indicate that the mechanism of resistance in ‘Kilaherb’ is different from that currently available in canola. Also, the intermediate phenotype of ‘B-2819’ may represent a quantitative resistance reaction that may also differ from that in canola.

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Acknowledgments

We thank the field crew of the Muck Crops Research Station for their technical assistance, and the Clubroot Risk Mitigation Initiative of Agriculture and Agri-Food Canada and the Canola Council of Canada for their financial support.

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Authors and Affiliations

  1. Department of Plant Agriculture, University of Guelph, 50 Stone Road East, N1G 2 W1, Guelph, ON, Canada

    T. V. Gludovacz, A. Deora & M. R. McDonald

  2. Agriculture and Agri-Food Canada, 107 Science Place, S7N 0X2, Saskatoon, SK, Canada

    B. D. Gossen

Authors
  1. T. V. Gludovacz
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  2. A. Deora
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  3. M. R. McDonald
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  4. B. D. Gossen
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Correspondence to B. D. Gossen.

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Gludovacz, T.V., Deora, A., McDonald, M.R. et al. Cortical Colonization by Plasmodiophora brassicae in Susceptible and Resistant Cabbage Cultivars. Eur J Plant Pathol 140, 859–862 (2014). https://doi.org/10.1007/s10658-014-0492-8

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  • DOI: https://doi.org/10.1007/s10658-014-0492-8

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