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Molecular diversity of Radopholus similis (Cobb 1913) Thorne (1949) (Nematoda: Pratylenchidae) affecting banana from Costa Rica

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Abstract

Radopholus similis is one of the most important diseases in banana in tropical/subtropical regions worldwide. The knowledge of its molecular diversity in specific areas could help to understand its distribution, ways of expansion, control methods, differential pathogenicity, and sustainable plant breeding programs. This study explores the molecular diversity of R. similis in an extensive survey in banana plantations in Costa Rica using two molecular markers, one genomic (ITS region) and another mitochondrial (COI). The results showed a low molecular variability, being dominated by a prevalent haplotype in both markers. Three haplotypes have been detected for each molecular marker respectively. The haplotypes present in Costa Rica are similar to others found in other areas of Central America (Colombia, Panama) and these haplotypes are also distributed worldwide. These results suggested a unique or limited number of introductions of this pathogen in Central America and a possible expansion within the continent by infected plant material.

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Fig. 1
Fig. 2

Data availability

R. similis GenBank accession number sequences: ON479314-ON479316 and ON479582-ON479584.

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Acknowledgements

The authors thank further anonymous reviewers and editors for their effort in reviewing the manuscript and helping to improve this study.

Authors’ contributions (optional: Please review the submission guidelines from the journal whether statements are mandatory)

All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by all authors. All authors read and approved the final manuscript.

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Authors and Affiliations

  1. Corporación Bananera Nacional – CORBANA S.A. – Dirección de Investigaciones, Sección Relación Suelo-Planta, Área de Nematología, Apdo. 390-7210, La Rita, Cantón de Pococí, Costa Rica

    Pedro E. Torres & Rafael Segura

  2. Doctorado en ciencias Naturales para el Desarrollo (DOCINADE), Instituto Tecnológico de Costa Rica, Universidad Nacional, Universidad Estatal a Distancia, APDO. 223-21001, Alajuela, San Carlos, Ciudad Quesada, Costa Rica

    Pedro E. Torres

  3. Independent Consultant, Turrialba, 30501, Costa Rica

    Jorge Sandoval

  4. Institute for Sustainable Agriculture (IAS), Spanish National Research Council (CSIC), Avenida Menéndez Pidal s/n, Campus de Excelencia Internacional Agroalimentario, ceiA3, 14004, Córdoba, Spain

    Carolina Cantalapiedra-Navarrete, Ilenia Clavero-Camacho, Pablo Castillo & Juan E. Palomares-Rius

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  1. Pedro E. Torres
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  2. Rafael Segura
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  3. Jorge Sandoval
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  4. Carolina Cantalapiedra-Navarrete
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  7. Juan E. Palomares-Rius
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Correspondence to Juan E. Palomares-Rius.

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All authors certify that 1) they do not have any actual or potential conflict of interest, 2) the study described is original and has not been published previously, and is not under consideration for publication elsewhere, 3) all prevailing local, national and international regulations and conventions, and normal scientific ethical practices, have been respected.

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The authors certify that 1) no special permits were required for the fieldwork investigations, 2) soil samplings did not involve any species endangered or protected in Costa Rica, 3) the soil samples analyzed in this study were obtained farming areas that are not under protection in any way.

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Torres, P.E., Segura, R., Sandoval, J. et al. Molecular diversity of Radopholus similis (Cobb 1913) Thorne (1949) (Nematoda: Pratylenchidae) affecting banana from Costa Rica. Eur J Plant Pathol 164, 439–446 (2022). https://doi.org/10.1007/s10658-022-02558-y

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