Abstract
Six populations (Parent 1, Parent 2, F1, F2, BC1 and BC2) generated from each of four crosses involving four resistant and two susceptible varieties of cowpea (Vigna unguiculata L. Walp) were evaluated for resistance to Cercospora leaf spot (CLS) disease caused by Pseudocercospora cruenta under induced epiphytotic conditions, in four separate field experiments. Climatic conditions determined the onset of CLS disease in the susceptible cultivar and varied in the four experiments from 35 to 48 days after planting (DAP). Genetic analysis revealed that the mode of inheritance of resistance to P. cruenta can be oligogenic or polygenic depending upon the cross. This is the first report of polygenic inheritance of CLS resistance. Number of nodes infected fitted a simple additive dominance model with predominance of additive effects based on generation mean analysis. Oligogenic resistance was observed for the other three crosses, with the most plausible models being: a single gene model with incomplete dominance in CB27 × IT87D-939-1; a single gene model with complete dominance in CB27 × VRB-10; and a triger model in Los Banos Bush Sitao × IT86D-792, based on segregation analysis of symptomatic : non-symptomatic plants. The role of minor genes was also indicated in the above crosses. Suggested approaches to breeding for resistance to CLS are discussed.
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References
Allen CJ (1983) The pathology of tropical food legumes. Wiley, Chichester, UK
Baker RED, Dale WT (1951) Fungi of Trinidad and Tobago. Anonymous. The Commonwealth Mycological Institute. Mycological Papers 33, Anonymous, Kew, Surrey, UK
Booker HM (2006) Studies on resistance to Cercospora leaf spot (CLS) disease and the impact of CLS and Cowpea severe mosaic virus (CPSMV) diseases, the productivity of Vigna unguiculata (L.) Walp. In Trinidad. Ph.D. thesis. The University of West Indies, St. Augustine, Trinidad and Tobago, WI
Booker HM, Umaharan P (2007) Identification of identification of resistance to Cercospora leaf spot (CLS) of cowpea. Eur J Plant Pathol doi: 10-1007/s10658-007-9152-6
Braun U (1995) A monograph of Cercosporella, Ramularia and allied genera (phytopathogenic hyphomycetes), vol 1. IHW-Verlag, Eching bei München, Germany
Braun U, Mouchacca J, McKenzie EHC (1999) Cercosporoid hyphomycetes from New Caledonia and some other South Pacific Islands. New Zeal J Bot 37:297–327
Brown CB, Bally GS (1970) Land capability survey of Trinidad and Tobago: Central Range, No. 4, Port of Spain, Trinidad, Government Printery
Castro NR, Menezes GC, Coelho RSB (2003) Inheritance of the genetic resistance of caupi to cercosporiose. Fitopathology 28(5):552–554
Cavali LL (1952) An analysis of linkage in quantitative inheritance. In: Reeve ECR, Waddington CJ (eds) Quantitative inheritance. HMSO, London, UK, pp 135–144
Crous PW, Braun U (2003) Mycospaerella and its anamorphs: 1. Names published in Cercospora and Passalora. Centraalbureau voor Schimmelcultures (CBS), Utrecht, The Netherlands
Deighton FC (1976) Studies on Cercospora and allied genera. VI. Psuedocercospora Speg., Pantospora Cif. and Cercoseptoria Petr. Mycol Pap 140:1–168
Fery RL, Dukes PD, Cuthbert FP Jr (1976) The inheritance of Cercospora leaf spot resistance in Southernpea (Vigna unguiculata (L.) Walp.). J Hortic Sci 101(2):148–149
Fery RL, Dukes PD (1977) An assessment of two genes for Cercospora leaf spot resistance in the Southernpea (Vigna unguiculata (L.) Walp.). J Hortic Sci 12(5):454–456
Fery RL, Dukes PD, Cuthbert FP Jr (1977) Yield-loss of Southern Pea (Vigna unguiculata) caused by Cercospora leaf spot. Plant Dis Rep 61(9):741–743
Hart RO (1977) Etiology of Cercospora cruenta Sacc. on Vigna unguiculata (L.) Walp. M.Sc. Thesis. The University of Guyana, Turkeyen, E.C.D. Guyana
Hayman BI (1960) Maximum likelihood estimation of genetic components of variation. Biometrics 16:369–381
Jhooty JS, Sokhi SS, Sohi HS, Bains SS, Rewal HS, Mushi GD (1980) The reaction of cowpea germplasm to different diseases. Crop Improv 7(1):25–31
Kannaiyan J, Greenberg DC, Haciwa HC, Mbewe MN (1987) Screening cowpea for resistance to major diseases in Zambia. Trop Grain Bull 34:23–26
Jinks JL, Jones RM (1958) Estimation of components of heterosis. Genetics 43:223–224
Kearsey MJ, Pooni HS (1996) The genetical analysis of quantitative traits, 1st edn. Chapman and Hall, London, UK
Lin MC, Chen HP, Wang YF, Zhang WZ (1995) Evaluation of cowpea varieties resistant to cowpea leaf mould (Cercospora cruenta Sacc.). Crop Genet Resour 4:36–37
Mather K (1949) Biometrical genetics, 1st edn. Methuen, London
Mather K, Jinks JL (1982) Biometrical genetics, 3rd edn. Chapman and Hall, London, UK
Mulder JL, Holliday P (1975) Cercospora cruenta. CMI Descriptions of Pathogenic Fungi and Bacteria No. 463
Mishra SP, Asthana AN, Yadav L (1988) Inheritance of Cercospora leaf spot resistance in Mung Bean, Vigna radiata (L.) Wilczek. Plant Breed 100:228–229
Quedraogo JT, Gowda BS, Jean M, Close TJ, Ehlers JD, Hall AE, Gillaspie AG, Roberts PA, Ismail AM, Bruening G, Gepts P, Timko MP, Belzile FJ (2002) An improved genetic linkage map for cowpea (Vigna unguiculata L.) Combining AFLP, RFLP, RAPD, biochemical markers, and biological resistance traits. Genome 45:175–188
Rachie KO, Bawal K, Franckowiok JK (1975) A rapid method of hand crossing cowpea. Technical Bulletin 2, International Institute of Tropical Agriculture, Ibadan, Nigeria
Rosenkranz E, Scott GE (1984) Determination of the number of genes for resistance to maize dwarf mosaic virus strain a in five corn inbred lines. Phytopathology 74(1):71–76
Schneider RW, Williams RJ, Sinclair JB (1976) Cercospora leaf spot of cowpea: models for estimating yield loss. Phytopathology 66:384–388
Scott GE, Rosenkranz E (1982) A method to determine the number of genes for resistance to maize dwarf mosaic in maize. Crop Sci 22:756–761
Singh RK, Chaudhary BD (1977) Biometrical methods in quantitative genetic analysis. Kanyani publishers, Ludhiana, India, 2nd edn, 1979
Thakur RP, Patel PN, Verma JP (2002) Genetical relationships between reactions to bacterial leaf spot, yellow mosaic and Cercospora leaf spot in mungbean (Vigna radiata). Euphytica 26:765–774
Umaharan P, Ariyanayagam RP, Haque SQ (1997) Response of cowpea genotypes [Vigna unguiculata (L.) Walp.] to artificial pollination in the tropics. Trop Agric (Trinidad), 74(1):39–44
Vakili NG (1977) Field screening of cowpeas for Cercospora leaf spot resistance. Trop Agric (Trinidad) 54(1):70–76
Williams RJ (1975) Diseases of cowpea (Vigna unguiculata (L.) Walp.) in Nigeria. Proc Natl Acad Sci USA 21(3):253–267
Williams RJ (1977) Identification of multiple disease resistance in cowpea. Trop Agric (Trinidad) 54(1):53–59
Zhang WZ, Wang YF, Lin MC (1994) Genetic distance estimation and cluster analysis of some yardlong bean resources. Acta Hortic Sin 21(2):180–184
Acknowledgements
The authors would like to thank the following: Professor Uwe Braun; Prof. Pedro W. Crous and Dr. John C. David for their assistance in identification of the CLS pathogens; Dr. A. E. Hall for providing seed for cowpea line CB27 and Dr. C. A. Fatokun for providing seed of the ‘IT’ cowpea lines. The financial assistance provided to the senior author by the Government of Trinidad and Tobago, under the Dean’s Scholarship for postgraduate study is gratefully acknowledged.
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Booker, H.M., Umaharan, P. Quantitative resistance to Cercospora leaf spot disease caused by Pseudocercospora cruenta in cowpea. Euphytica 162, 167–177 (2008). https://doi.org/10.1007/s10681-007-9490-7
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DOI: https://doi.org/10.1007/s10681-007-9490-7