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Efficacy of mozuku fucoidan in alternative protein-based diet to improve growth, health performance, and stress resistance of juvenile red sea bream, Pagrus major

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Abstract

We evaluated the efficacy of mozuku fucoidan supplementation to alternative dietary proteins used in fish meal (FM) replacement to enhance growth, immunity, and stress resistance of Pagrus major. Seven isonitrogenous (45% protein) experimental diets were formulated where diet 1 (D1) was FM-based control diet. Diets 2 to 7 were formulated by replacing 25, 50, and 75% of FM protein with soy protein isolate (SPI) protein, and each replacement level was supplemented without or with fucoidan at 0.4% for diet groups D2 (FM25), D3 (FM25Fu), D4 (FM50), D5 (FM50Fu), D6 (FM75), and D7(FM75Fu), respectively. Each diet was randomly allocated to triplicate groups of fish (4.1 g) for 56 days. Significantly higher weight gain and specific growth rate were observed in fish fed FM50Fu diet group, and it was not differed (P > 0.05) with fish fed FM25Fu diet group. FM-based control diet showed intermediate value, and it was not differed (P > 0.05) with or without fucoidan-supplemented ≤ 50% FM replacement groups and FM75Fu diet group. Significantly lower growth performances were observed in FM75 diet group. At each replacement level, fucoidan-supplemented groups showed nonsignificant improvement of feed utilization performances. Fish fed fucoidan-supplemented diets showed best condition of oxidative and freshwater stress resistance. Lysozyme activity, NBT, and peroxidase activity showed higher (P > 0.05) values in fucoidan-supplemented groups compared with the non-supplemented groups. Catalase activity was significantly lower in FM75Fu diet group. Catalase activity is significantly influenced by the interaction effects of fucoidan and FM replacement level. In conclusion, fucoidan supplementation could increase the efficiency of utilizing SPI (≥ 75%) without any adverse effects on red sea bream performance.

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Acknowledgments

Thanks are also due to Mr. Serge Dossou, Mr. Kokubu, and Mr. Taniguchi for their technical and logistic support during feeding trial and sample analysis.

Funding

This study was supported by the Management Expenses Grants of the United Graduate School of Agricultural Sciences, Kagoshima University, provided to Dr. Shunsuke Koshio.

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  1. Md. Sakhawat Hossain

    Present address: Hagerman Fish Culture Experiment Station, Aquaculture Research Institute, University of Idaho, 3059F National Fish Hatchery Road, Hagerman, ID, 83332, USA

Authors and Affiliations

  1. The Graduate School of Fisheries, Kagoshima University, Shimoarata 4-50-20, Kagoshima, 890-0065, Japan

    Nadia Mahjabin Sony, Manabu Ishikawa & Shunsuke Koshio

  2. Laboratory of Aquatic Animal Nutrition, Faculty of Fisheries, Kagoshima University, Shimoarata 4-50-20, Kagoshima, 890-0056, Japan

    Nadia Mahjabin Sony, Md. Sakhawat Hossain, Manabu Ishikawa, Shunsuke Koshio & Saichiro Yokoyama

  3. Department of Aquaculture, Faculty of Fisheries, Sylhet Agricultural University, -3100, Sylhet, Bangladesh

    Md. Sakhawat Hossain

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Sony, N.M., Hossain, M.S., Ishikawa, M. et al. Efficacy of mozuku fucoidan in alternative protein-based diet to improve growth, health performance, and stress resistance of juvenile red sea bream, Pagrus major. Fish Physiol Biochem 46, 2437–2455 (2020). https://doi.org/10.1007/s10695-020-00881-x

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