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Identification of lipid raft glycoproteins obtained from boar spermatozoa

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Abstract

The surface of the spermatozoa is coated with glycoproteins the redistribution of which during in vitro capacitation plays a key role in the subsequent fertilization process. Lipid rafts are membrane microdomains involved in signal transduction through receptors and include or recruit specific types of proteins and glycoproteins. Few studies have focused on identifying glycoproteins resident in the lipid rafts of spermatozoa. Proteins associated with lipid rafts modify their localization during capacitation. The objective of the study was to identify the glycoproteins associated with lipid rafts of capacitated boar spermatozoa through a lectin-binding assay coupled to mass spectrometry approach. From the proteomic profiles generated by the raft proteins extractions, we observed that after capacitation the intensity of some bands increased while that of others decreased. To determine whether the proteins obtained from lipid rafts are glycosylated, lectin blot assays were performed. Protein bands with a good resolution and showing significant glycosylation modifications after capacitation were analyzed by mass spectrometry. The bands of interest had an apparent molecular weight of 64, 45, 36, 34, 24, 18 and 15 kDa. We sequenced the 7 bands and 20 known or potential glycoproteins were identified. According to us, for ten of them this is the first time that their association with sperm lipid rafts is described (ADAM5, SPMI, SPACA1, Seminal plasma protein pB1, PSP-I, MFGE8, tACE, PGK2, SUCLA2, MDH1). Moreover, LYDP4, SPAM-1, HSP60, ZPBP1, AK1 were previously reported in lipid rafts of mouse and human spermatozoa but not in boar spermatozoa. We also found and confirmed the presence of ACR, ACRBP, AWN, AQN3 and PRDX5 in lipid rafts of boar spermatozoa. This paper provides an overview of the glycosylation pattern in lipid rafts of boar spermatozoa before and after capacitation. Further glycomic analysis is needed to determine the type and the variation of glycan chains of the lipid rafts glycoproteins on the surface of spermatozoa during capacitation and acrosome reaction.

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List of abbreviations

AR: acrosome reaction; C: capacitated; DIG: digoxigenin; DRM: detergent-resistant membrane; DSA: Datura stramonium agglutinin; Gal: galactose; GlcNAc: N-acetylglucosamine; GM1: ganglioside M1; GNA: Galanthus nivalis agglutinin; Man: mannose; NC: non-capacitated; NeuAc: sialic acid; OD: optical density; PNA: peanut agglutinin; RP: raft proteins; RPC: raft protein of C; RPNC: raft protein of NC; SNA: Sambucus nigra agglutinin; TPC: total protein of capacitated spermatozoa; TPNC: total protein of non-capacitated spermatozoa; WGA: wheat germ agglutinin; ZP: zona pellucida.

Acknowledgments

We thank Yobana Pérez-Cervera, Ludivine Drougat, Ikram El Yazidi Belkoura and Marlène Mortuaire for technical assistance.

Funding

This work was partially supported by CONACYT, México (grant number 105961-M), scholarship to JBLS (number 233535), Bilateral Cooperation Projects Mexico-France CONACYT-ANUIES-ECOS (grant number M10-A02). The Proteomics facility is funded by the European Community (FEDER), the “Région Nord-Pas de Calais”, the IBISA network, the CNRS, and the University of Lille-Faculté des Sciences et Technologies.

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Authors and Affiliations

  1. Doctorado en Ciencias Biológicas y de la Salud, Universidad Autónoma Metropolitana, Ciudad de México, México

    José Benito López-Salguero

  2. Departamento de Ciencias de la Salud. D.C.B.S, Universidad Autónoma Metropolitana-Iztapalapa, San Rafael Atlixco 186, Col. Vicentina, C.P, 09340, Ciudad de México, México

    Reyna Fierro, Irma Jiménez-Morales & Humberto González-Márquez

  3. Univ. Lille, CNRS, UMR 8576 - UGSF - Unité de Glycobiologie Structurale et Fonctionnelle, F-59000, Lille, France

    Jean-Claude Michalski, Tony Lefebvre, Steffi F. Baldini & Anne-Sophie Vercoutter-Edouart

  4. Maestría en Biología Experimental, Universidad Autónoma Metropolitana-Iztapalapa, Ciudad de México, México

    Oscar Mondragón-Payne

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  1. José Benito López-Salguero
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  2. Reyna Fierro
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Contributions

Direction of the study and established the experimental design: RF, IJM, HGM, ASVE, TL, JCM. Performed the experiments: JBLS, OMP, SFB. All authors analyzed the results, contributed to the finalized manuscript and approved the manuscript.

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Correspondence to Reyna Fierro.

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López-Salguero, J.B., Fierro, R., Michalski, JC. et al. Identification of lipid raft glycoproteins obtained from boar spermatozoa. Glycoconj J 37, 499–509 (2020). https://doi.org/10.1007/s10719-020-09924-0

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  • DOI: https://doi.org/10.1007/s10719-020-09924-0

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