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Kiwi fruit PMEI inhibits PME activity, modulates root elongation and induces pollen tube burst in Arabidopsis thaliana

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Abstract

Pectins are major components of primary cell wall that play a crucial role in plant development. After biosynthesis, pectins are secreted in the cell wall by Golgi-derived vesicles under a highly methylesterified form and are de-methylesterified by pectin methylesterases (PME). It is hypothesized that PME might be regulated by pectin methylesterase inhibitor (PMEI). In this paper, we show by isoelectric focalisation and subsequent zymogram that kiwi PMEI was able to inhibit Arabidopsis PME activity by forming a complex. The complexes were stable under a wide range of ionic strength and pH. Moreover, PMEI might be able to form a complex with basic PMEs including three PMEs strongly expressed in root and four PMEs expressed in pollen grains. Finally, exogenous treatment with kiwi PMEI was able to reduce the activity of cell wall resident PMEs with persistent effects such as an increase of the root growth and a dramatic effect on pollen tube stability.

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Abbreviations

HG:

Homogalacturonan

IEF:

Isoelectric focalisation

PME:

Pectin methylesterase

PMEI:

Pectin methylesterase inhibitor

RG-I:

Rhamnogalacturonan-I

RG-II:

Rhamnogalacturonan-II

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Acknowledgments

The authors are grateful to Anne Gée for her excellent technical assistance and the University of Rouen for its constant logistic helps. This work was supported by the ANR (ANR-09-BLANC-0007-01 Project GROWPEC). This work was supported by the University of Rouen and the “Trans Channel Wallnet” project that has been selected in the context of the INTERREG IVA France (Channel)—England European cross-border cooperation programme, which is co-financed by the ERDF.

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Authors and Affiliations

  1. Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale (Glyco-MEV) EA 4358, Normandy University, University of Rouen, Institut de Recherche et d’Innovation Biomédicale, 76821, Mont-Saint-Aignan, France

    Florence Paynel, Christelle Leroux, Ogier Surcouf, Azeddine Driouich, Jean Claude Mollet, Patrice Lerouge, Arnaud Lehner & Alain Mareck

  2. UMR-CNRS 6270, Normandy University, University of Rouen, Institut de Recherche et d’Innovation Biomédicale, 76821, Mont-Saint-Aignan, France

    Annick Schaumann

  3. Laboratoire Biologie des Plantes et Innovation (BIOPI) EA3900, University of Picardie Jules Verne, 33 Rue St Leu, 80039, Amiens, France

    Jérôme Pelloux

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  1. Florence Paynel
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  2. Christelle Leroux
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  3. Ogier Surcouf
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  7. Jean Claude Mollet
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  9. Arnaud Lehner
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Correspondence to Arnaud Lehner.

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Arnaud Lehner and Alain Mareck have equal contribution of the senior authors.

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10725_2014_9919_MOESM1_ESM.tif

Fig S1 Electrophoresis gel of purified kiwi PMEI – (a) Molecular weight standards. (b) Purified kiwi PMEI (10 µg). (TIFF 3,477 kb)

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Paynel, F., Leroux, C., Surcouf, O. et al. Kiwi fruit PMEI inhibits PME activity, modulates root elongation and induces pollen tube burst in Arabidopsis thaliana . Plant Growth Regul 74, 285–297 (2014). https://doi.org/10.1007/s10725-014-9919-7

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