Abstract
Circumvallate papilla (CVP) is a distinctively structured with dome-shaped apex, and the surrounding trench which contains over two hundred taste buds on the lateral walls. Although CVP was extensively studied to determine the regulatory mechanisms during organogenesis, it still remains to be elucidated the principle mechanisms of signaling regulations on morphogenesis including taste buds formation. The key role of Yes-associated protein (YAP) in the regulation of organ size and cell proliferation in vertebrates is well understood, but little is known about the role of this signaling pathway in CVP development. We aimed to determine the putative roles of YAP signaling in the epithelial patterning during CVP morphogenesis. To evaluate the precise localization patterns of YAP and other related signaling molecules, including β-catenin, Ki67, cytokeratins, and PGP9.5, in CVP tissue, histology and immunohistochemistry were employed at E16 and adult mice. Our results suggested that there are specific localization patterns of YAP and Wnt signaling molecules in developing and adult CVP. These concrete localization patterns would provide putative involvements of YAP and Wnt signaling for proper epithelial cell differentiation including the formation and maintenance of taste buds.
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Acknowledgements
This work was supported by a grant from the National Research Foundation of Korea (NRF) funded by the Korean government (Grant No. NRF-2019R1I1A3A01062091).
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JYK and TYK contributed to conception, data acquisition, analysis and interpretation, drafted and revised the manuscript. ESL and YPA contributed to the analysis and interpretation of data. EP, SS, and WJS contributed to the analysis and quantification of data. JYK and JKJ contributed to conception, design, analysis and data interpretation and critically revised the manuscript.
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Kim, JY., Kim, TY., Lee, ES. et al. Implications of the specific localization of YAP signaling on the epithelial patterning of circumvallate papilla. J Mol Histol 52, 313–320 (2021). https://doi.org/10.1007/s10735-020-09951-z
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DOI: https://doi.org/10.1007/s10735-020-09951-z