Abstract
Mesenchymal stem cells (MSCs) are considered a promising tool for treating cerebral ischemic injury. However, their poor survival after transplantation limits their therapeutic effect and applications. Salidroside has been reported to exert potent cytoprotective and neuroprotective effects. This study aimed to investigate whether salidroside could improve MSC survival under hypoxic-ischemic conditions and, subsequently, alleviate cerebral ischemic injury in a rat model. MSCs were pretreated by salidroside under hypoxic-ischemic conditions. The cell proliferation, migratory capacity, and apoptosis were evaluated by means of Cell Counting Kit-8, transwell assay, and flow cytometry. MSCs pretreated with salidroside were transplanted into the rats subsequent to middle cerebral artery occlusion. The grip strength, 2,3,5-triphenyltetrazolium chloride, and hematoxylin–eosin staining were used to analyze the therapeutic efficiency and pathological changes. The mature neuron marker NeuN and astrocyte marker GFAP in the focal area were detected by immunofluorescence. These results indicated that salidroside promoted the proliferation, migration and reduced apoptosis of MSCs under hypoxic-ischemic conditions. In vivo experiments revealed that transplantation of salidroside-pretreated MSCs strengthened the therapeutic efficiency by enhancing neurogenesis and inhibiting neuroinflammation in the hippocampal CA1 area after ischemia. Our results suggest that pretreatment with salidroside could be an effective strategy to enhance the cell survival rate and the therapeutic effect of MSCs in treating cerebral ischemic injury.
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The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.
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This work was financially supported by the National Natural Science Foundation of China (31570994); Project of Zhejiang Education Department (Y20163679).
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QY conceived the idea and designed the experiments. LPZ and PPY performed the experiments and wrote the manuscript. LXJ performed the animal experiments. ZYW and XHM performed the cellular experiment. GXW and JTY participated in part of the animal experiments. BJZ collected and analyzed the data. All authors read and approved the final manuscript.
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Zhou, L., Yao, P., Jiang, L. et al. Salidroside-pretreated mesenchymal stem cells contribute to neuroprotection in cerebral ischemic injury in vitro and in vivo. J Mol Histol 52, 1145–1154 (2021). https://doi.org/10.1007/s10735-021-10022-0
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DOI: https://doi.org/10.1007/s10735-021-10022-0