Abstract
Background
Curcuma longa has been well documented for managing joint inflammation and pain. The present study investigated the effect of polar extract of C. longa (NR-INF-02) on cartilage homeostasis in human articular chondrocytes knee (NHAC-kn) cells to understand its plausible mechanism of action.
Methods
Dysregulation of cartilage homeostasis was induced by IL-1β and H2O2. Modulating effects of NR-INF-02 on degradation markers viz., chondrocyte apoptosis, senescence, cytokine, eicosanoids, and cartilage synthesis markers viz., glycosaminoglycans and type II collagen degradation was evaluated in human articular chondrocytes knee (NHAC-kn) cells. Further, the effect of NR-INF-02 on lipopolysaccharide (LPS)-induced expression of NF-kB in RAW264.7 macrophages was investigated.
Results
NR-INF-02 significantly attenuated IL-1β-induced chondrocyte cytotoxicity, apoptosis and release of chondrocyte degradation markers such as IL-6, IL-8, COX-2, PGE2, TNF-α, ICAM-1 in NHAC-kn cells. Also, NR-INF-02 protected IL-1β-induced damage to synthesis markers such as glycosaminoglycans, type II collagen and further attenuated H2O2-induced chondrocyte senescence. In addition NR-INF-02 suppressed LPS-induced NF-kB expression in RAW264.7 cells.
Conclusions
NR-INF-02 protects cartilage homeostasis by maintaining the balance between synthesis and degradation of cartilage matrix.
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Acknowledgements
The authors are thankful to Indo–Spanish Joint Programme, Department of Biotechnology (DBT) of India, Centre for the development of Industrial Technology (CDTI) of Spain for providing partial financial assistance to this study.
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Velusami, C.C., Richard, E.J. & Bethapudi, B. Polar extract of Curcuma longa protects cartilage homeostasis: possible mechanism of action. Inflammopharmacol 26, 1233–1243 (2018). https://doi.org/10.1007/s10787-017-0433-1
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DOI: https://doi.org/10.1007/s10787-017-0433-1