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The complete plastid genomes of Betaphycus gelatinus, Eucheuma denticulatum, and Kappaphycus striatus (Solieriaceae: Rhodophyta) and their phylogenetic analysis

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Abstract

Betaphycus, Eucheuma and Kappaphycus (Gigartinales, Florideophyceae) are the most commercially important genera of the family Solieriaceae that produce carrageenan. Here, three complete plastid genomes of Betaphycus gelatinus, Eucheuma denticulatum and Kappaphycus striatus were fully sequenced using next-generation sequencing technology. Genome organizations and gene contents of the three plastid genomes were highly alike. They all had circular mapping organizations and the sizes were 178,394 bp (B. gelatinus), 177,003 bp (E. denticulatum), and 176,763 bp (K. striatus). They encoded almost the same set of plastid genes (238–240), including 202 to 204 protein-encoding genes, 30 transfer RNA genes (tRNAs), 3 ribosomal RNA genes (rRNAs), 2 misc_RNAs (ffs, rnpB), and 1 transfer-messenger RNA gene (tmRNA). One group II intron interrupting the trnMe gene was identified in each of these three plastid genomes. Other three plastid genomes from species of the order Gigartinales including Kappaphycus alvarezii, Chondrus crispus and Mastocarpus papillatus have been reported. The plastid genome organization at the level of the order Gigartinales was highly conserved. Co-linear analysis among the six plastid genomes of the Gigartinales showed the considerable sequence synteny with the exception of one remarkable gene rearrangement. The approximately 12.5-kb gene fragment from gene psaM to ycf21 in plastid genomes of the four species of the Solieriaceae was completely reversed compared to that of M. papillatus and C. crispus. It might be used as the potential phylogenetic markers uniting the species of the Solieriaceae. In addition, phylogenetic analysis based on 138 shared protein-encoding genes from 53 Florideophyceae plastid genomes indicated all species were clearly divided into five clades corresponding to their subclasses. The results suggested there was a non-monophyletic relationship of the order Gigartinales. Four species of the family Solieriaceae formed one clade and E. denticulatum was basal relative to the other three species. The novel plastid genomes expand the available plastid pool for red algae which would facilitate the phylogenetic study in algae.

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Funding

This work was supported by the China-ASEAN Maritime Cooperation Fund; Public Science and Technology Research Funds Projects of Ocean (Grant No. 201405020); the Natural Science Foundation of Shandong Province (Grant No. ZR2019MC049).

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  1. Jing Zhang and Na Liu contributed equally to this work.

Authors and Affiliations

  1. School of Bioengineering, Qilu University of Technology (Shandong Academy of Sciences), 3501 Daxue Road, Jinan, 250353, Shandong Province, China

    Jing Zhang

  2. College of Marine Life Sciences, Ocean University of China, 5 Yushan Road, Qingdao, 266003, Shandong Province, China

    Na Liu, Yuemei Jin & Tao Liu

  3. College of Life Sciences, Beijing Normal University, 19 Xinjiekouwai Road, Beijing, 100875, China

    Na Liu

  4. National Institute of Biological Sciences, 7 Kexueyuan Road, Beijing, 102206, China

    Na Liu

  5. Fisheries and Marine Science, Jenderal Soedirman University, Purwokerto, 53122, Indonesia

    Maria Dyah Nur Meinita

  6. College of Life Sciences, Yantai University, 30 Qingshan Road, Yantai, 264005, Shandong Province, China

    Xumin Wang

  7. Hainan Academy of Ocean and Fisheries Sciences, 54 Haifu Road, Haikou, 570203, Hainan Province, China

    Xianming Tang

  8. Beijing Academy of Agriculture and Forestry Sciences, 11 Shuguanghuayuan Road, Beijing, 100097, China

    Guoliang Wang

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  1. Jing Zhang
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Zhang, J., Liu, N., Meinita, M.D.N. et al. The complete plastid genomes of Betaphycus gelatinus, Eucheuma denticulatum, and Kappaphycus striatus (Solieriaceae: Rhodophyta) and their phylogenetic analysis. J Appl Phycol 32, 3521–3532 (2020). https://doi.org/10.1007/s10811-020-02120-5

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