Skip to main content

Advertisement

Log in

Investigating Isotopic Niche Space: Using rKIN for Stable Isotope Studies in Archaeology

  • Published:
Journal of Archaeological Method and Theory Aims and scope Submit manuscript

This article has been updated

Abstract

Archaeological applications of stable isotope data have become increasingly prevalent, and the use of these data continues to expand rapidly. Researchers are starting to find that recovering data for multiple elements provides additional insight and quantitative data for answering questions about past human activities and behaviors. Many stable isotope studies in archaeology, however, rarely move beyond comparisons of descriptive statistics such as mean, median, and standard deviation. Over the last decade, ecologists have formalized the concept of isotopic niche space, and corresponding isotopic niche overlap, to incorporate data from two or more isotopic systems into a single analysis. Additionally, several methods for quantifying isotopic niche space and overlap are now available. Here, I describe the evolution of the isotopic niche space concept and demonstrate the usefulness of it for archaeological research through three case studies using the recently developed rKIN package that allows for a comparison of different methods of isotopic niche space and overlap estimations. Two case studies apply these new measures to previously published studies, while a third case study illustrates its applicability to exploring new hypotheses and research directions. The benefits and limitations of quantifying isotopic niche space and overlap are discussed, as well as suggestions for data reporting and transparency when using these methods. Isotopic niche space and overlap metrics will allow archaeologists to extract more nuanced information from stable isotope datasets in their drive to understand more fully the histories of the human conditions.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

Change history

  • 15 November 2021

    The original version of this paper was updated due to the revised statement of Availability of Data and Material

References

  • Albeke, S. E. (2017). rKIN:(kernel) isotope niche estimation. R package version 0.1.

  • Ambrose, S. H. (1991). Effects of diet, climate and physiology on nitrogen isotope abundances in terrestrial foodwebs. Journal of Archaeological Science, 18(3), 293–317.

    Article  Google Scholar 

  • Ambrose, S. H. (1993). Isotopic analysis of paleodiets: Methodological and interpretive consideration. In M. K. Sandford (Ed.), Investigations of ancient human tissue: Chemical analyses in anthropology (pp. 50-130). Gordon and Breach.

  • Ambrose, S. H., & DeNiro, M. (1986). Reconstruction of African human diet using bone collagen carbon and nitrogen isotope ratios. Nature, 319, 321–324.

    Article  Google Scholar 

  • Bentley, R. A. (2006). Strontium isotopes from the earth to the archaeological skeleton: A review. Journal of Archaeological Method and Theory, 13(3), 135–187.

    Article  Google Scholar 

  • Bentley, R. A., & Knipper, C. (2005). Geographical patterns in biologically available strontium, carbon and oxygen isotope signatures in prehistoric SW Germany. Archaeometry, 47(3), 629–644.

    Article  Google Scholar 

  • Blumenthal, S. A., Levin, N. E., Brown, F. H., Brugal, J. P., Chritz, K. L., Harris, J. M., Jehle, G. E., & Cerling, T. E. (2017). Aridity and hominin environments. Proceedings of the National Academy of Sciences, 114(28), 7331–7336.

    Article  Google Scholar 

  • Boyd, M., Feibel, C. S., Manthi, F. K., Ward, C. V., & Plavcan, J. M. (2018). A synthesis of multi-proxy paleoenvironmental reconstruction methods: The depositional environments of the Lomekwi Member, Nachukui Formation, West Turkana. In AGU Fall Meeting Abstracts (Vol. 2018, pp. PP31B-1657).

  • Brill, R. H., & Wampler, J. M. (1967). Isotope studies of ancient lead. American Journal of Archaeology, 71(1), 63–77.

    Article  Google Scholar 

  • Cerling, T.E. (2014). 14.12 Stable isotope evidence for hominin environments in Africa. Treatise on Geochemistry, Springer, pp. 158-166.

  • Cerling, T. E., Bowman, J. R., & O’Neil, J. R. (1988). An isotopic study of a fluvial-lacustrine sequence: The Plio-Pleistocene Koobi Fora sequence, East Africa. Palaeogeography, Palaeoclimatology, Palaeoecology, 63(4), 335–356.

    Article  Google Scholar 

  • Cerling, T. E., Harris, J. M., & Passey, B. H. (2003). Diets of East African Bovidae based on stable isotope analysis. Journal of Mammalogy, 84, 456–470.

    Article  Google Scholar 

  • Cerling, T. E., Manthi, F. K., Mbua, E. N., Leakey, L. N., Leakey, M. G., Leakey, R. E., Brown, F. H., Grine, F. E., Hart, J. A., Kaleme, P., Roche, H., Uno, K. T., & Wood, B. A. (2013). Stable isotope-based diet reconstructions of Turkana Basin hominins. Proceedings of the National Academy of Sciences, 110(26), 10501–10506.

    Article  Google Scholar 

  • Cheung, C., Jing, Z., Tang, J., Weston, D. A., & Richards, M. P. (2017). Diets, social roles, and geographical origins of sacrificial victims at the royal cemetery at Yinxu, Shang China: New evidence from stable carbon, nitrogen, and sulfur isotope analysis. Journal of Anthropological Archaeology, 48, 28–45.

    Article  Google Scholar 

  • Chritz, K. L., Marshall, F. B., Zagal, M. E., Kirera, F., & Cerling, T. E. (2015). Environments and trypanosomiasis risks for early herders in the later Holocene of the Lake Victoria basin, Kenya. Proceedings of the National Academy of Sciences, 112(12), 3674–3679.

    Article  Google Scholar 

  • DeNiro, M. J. (1987). Stable isotopy and archaeology. American Scientist, 75(2), 182–191.

    Google Scholar 

  • DeNiro, M. J., & Epstein, S. (1981). Influence of diet on the distribution of nitrogen isotopes in animals. Geochimica et cosmochimica acta, 45(3), 341–351.

    Article  Google Scholar 

  • Diefendorf, A. F., Mueller, K. E., Wing, S. L., Koch, P. L., & Freeman, K. H. (2010). Global patterns in leaf 13C discrimination and implications for studies of past and future climate. Proceedings of the National Academy of Sciences, 107, 5738–5743.

  • Du, A., Rowan, J., Wang, S. C., Wood, B. A., & Alemseged, Z. (2020). Statistical estimates of hominin origination and extinction dates: A case study examining the Australopithecus anamensis–afarensis lineage. Journal of Human Evolution, 138, 102688.

    Article  Google Scholar 

  • Dupras, T. L., Schwarcz, H. P., & Fairgrieve, S. I. (2001). Infant feeding and weaning practices in Roman Egypt. American Journal of Physical Anthropology, 115(3), 204–212.

    Article  Google Scholar 

  • Eckrich, C. A., Albeke, S. E., Flaherty, E. A., Bowyer, R. T., & Ben-David, M. (2020). rKIN: Kernel-based method for estimating isotopic niche size and overlap. Journal of Animal Ecology, 89(3), 757–771.

    Article  Google Scholar 

  • Ericson, J. E. (1985). Strontium isotope characterization in the study of prehistoric human ecology. Journal of Human Evolution, 14(5), 503–514.

    Article  Google Scholar 

  • Evans, J. A., Chenery, C. A., & Fitzpatrick, A. P. (2006). Bronze Age childhood migration of individuals near Stonehenge, revealed by strontium and oxygen isotope tooth enamel analysis. Archaeometry, 48(2), 309–321.

    Article  Google Scholar 

  • Faith, J. T. (2018). Paleodietary change and its implications for aridity indices derived from δ18O of herbivore tooth enamel. Palaeogeography, Palaeoclimatology, Palaeoecology, 490, 571–578.

    Article  Google Scholar 

  • Flaherty, E. A., & Ben-David, M. (2010). Overlap and partitioning of the ecological and isotopic niches. Oikos, 119(9), 1409–1416.

    Article  Google Scholar 

  • Galetti, M., Rodarte, R. R., Neves, C. L., Moreira, M., & Costa-Pereira, R. (2016). Trophic niche differentiation in rodents and marsupials revealed by stable isotopes. PLoS One, 11(4), e0152494.

    Article  Google Scholar 

  • Garrett, N. D., Fox, D. L., McNulty, K. P., Faith, J. T., Peppe, D. J., Van Plantinga, A., & Tryon, C. A. (2015). Stable isotope paleoecology of late Pleistocene middle stone age humans from the Lake Victoria Basin, Kenya. Journal of human evolution, 82, 1–14.

    Article  Google Scholar 

  • Haile-Selassie, Y., Gibert, L., Melillo, S. M., Ryan, T. M., Alene, M., Deino, A., Levin, N. E., Scott, G., & Saylor, B. Z. (2015). New species from Ethiopia further expands Middle Pliocene hominin diversity. Nature, 521(7553), 483–488.

    Article  Google Scholar 

  • Haile-Selassie, Y., Melillo, S. M., Vazzana, A., Benazzi, S., & Ryan, T. M. (2019). A 3.8-million-year-old hominin cranium from Woranso-Mille, Ethiopia. Nature, 573(7773), 214–219.

    Article  Google Scholar 

  • Harmand, S., Lewis, J. E., Feibel, C. S., Lepre, C. J., Prat, S., Lenoble, A., Roche, H., et al. (2015). 3.3-million-year-old stone tools from Lomekwi 3, West Turkana, Kenya. Nature, 521(7552), 310–315.

    Article  Google Scholar 

  • Hartman, G. (2011). Are elevated δ15N values in herbivores in hot and arid environments caused by diet or animal physiology? Functional Ecology, 25(1), 122–131.

    Article  Google Scholar 

  • Hedges, R. E., & Reynard, L. M. (2007). Nitrogen isotopes and the trophic level of humans in archaeology. Journal of archaeological science, 34(8), 1240–1251.

    Article  Google Scholar 

  • Hermes, T. R., Frachetti, M. D., Bullion, E. A., Maksudov, F., Mustafokulov, S., & Makarewicz, C. A. (2018). Urban and nomadic isotopic niches reveal dietary connectivities along Central Asia’s Silk Roads. Scientific Reports, 8(1), 1–11.

    Article  Google Scholar 

  • Hette-Tronquart, N. (2019). Isotopic niche is not equal to trophic niche. Ecology Letters, 22(11), 1987–1989.

    Article  Google Scholar 

  • Hutchinson, G. E. (1957). Concluding remarks. Cold Spring Harbor Symposia on Quantitative Biology, 22, 415–427.

    Article  Google Scholar 

  • Jackson, A. L., Inger, R., Parnell, A. C., & Bearhop, S. (2011). Comparing isotopic niche widths among and within communities: SIBER–Stable Isotope Bayesian Ellipses in R. Journal of Animal Ecology, 80(3), 595–602.

    Article  Google Scholar 

  • Jackson, M. C., & Britton, J. R. (2014). Divergence in the trophic niche of sympatric freshwater invaders. Biological Invasions, 16(5), 1095–1103.

    Article  Google Scholar 

  • Karlson, A. M., Gorokhova, E., & Elmgren, R. (2015). Do deposit-feeders compete? Isotopic niche analysis of an invasion in a species-poor system. Scientific Reports, 5(1), 1–8.

    Article  Google Scholar 

  • Kimbel, W. H., Lockwood, C. A., Ward, C. V., Leakey, M. G., Rak, Y., & Johanson, D. C. (2006). Was Australopithecus anamensis ancestral to A. afarensis? A case of anagenesis in the hominin fossil record. Journal of Human Evolution, 51(2), 134–152.

    Article  Google Scholar 

  • Klein, R. G. (2013). Stable carbon isotopes and human evolution. Proceedings of the National Academy of Sciences, 110(26), 10470–10472.

    Article  Google Scholar 

  • Kohn, M. J. (2010). Carbon isotope compositions of terrestrial C3 plants as indicators of (paleo) ecology and (paleo)climate. Proceedings of the National Academy of Sciences, 107, 19691–19695.

  • Larsen, C. S., Schoeninger, M. J., Van der Merwe, N. J., Moore, K. M., & Lee-Thorp, J. A. (1992). Carbon and nitrogen stable isotopic signatures of human dietary change in the Georgia Bight. American Journal of Physical Anthropology, 89(2), 197–214.

    Article  Google Scholar 

  • Layman, C. A., Arrington, D. A., Montaña, C. G., & Post, D. M. (2007). Can stable isotope ratios provide for community-wide measures of trophic structure? Ecology, 88(1), 42–48.

    Article  Google Scholar 

  • Levin, N. E., Cerling, T. E., Passey, B. H., Harris, J. M., & Ehleringer, J. R. (2006). A stable isotope aridity index for terrestrial environments. Proceedings of the National Academy of Sciences, 103(30), 11201–11205.

    Article  Google Scholar 

  • Levin, N. E., Zipser, E. J., & Cerling, T. E. (2009). Isotopic composition of waters from Ethiopia and Kenya: Insights into moisture sources for eastern Africa. Journal of Geophysical Research: Atmospheres, 114(D23).

  • Levin, N. E., Haile-Selassie, Y., Frost, S. R., & Saylor, B. Z. (2015). Dietary change among hominins and cercopithecids in Ethiopia during the early Pliocene. Proceedings of the National Academy of Sciences, 112(40), 12304–12309.

    Article  Google Scholar 

  • Loponte, D., & Corriale, M. J. (2020). Patterns of resource use and isotopic niche overlap among guanaco (Lama guanicoe), pampas deer (Ozotoceros bezoarticus) and marsh deer (Blastocerus dichotomus) in the pampas. Ecological, paleoenvironmental and archaeological implications. Environmental Archaeology, 25(4), 411–444.

    Article  Google Scholar 

  • Martin, J. E., Tacail, T., Braga, J., Cerling, T. E., & Balter, V. (2020). Calcium isotopic ecology of Turkana Basin hominins. Nature Communications, 11(1), 1–7.

    Article  Google Scholar 

  • Martínez del Rio, C., Wolf, N., Carleton, S. A., & Gannes, L. Z. (2009). Isotopic ecology ten years after a call for more laboratory experiments. Biological Reviews, 84(1), 91–111.

    Article  Google Scholar 

  • Newsome, S. D., Martinez del Rio, C., Bearhop, S., & Phillips, D. L. (2007). A niche for isotopic ecology. Frontiers in Ecology and the Environment, 5(8), 429–436.

    Article  Google Scholar 

  • Newsome, S. D., Yeakel, J. D., Wheatley, P. V., & Tinker, M. T. (2012). Tools for quantifying isotopic niche space and dietary variation at the individual and population level. Journal of Mammalogy, 93(2), 329–341.

    Article  Google Scholar 

  • Patterson, D. B., Braun, D. R., Allen, K., Barr, W. A., Behrensmeyer, A. K., Biernat, M., Lehmann, S. B., Maddox, T., Manthi, F. K., Merritt, S. R., Morris, S. E., O’Brien, K., Reeves, J. S., Wood, B. A., & Bobe, R. (2019). Comparative isotopic evidence from East Turkana supports a dietary shift within the genus Homo. Nature Ecology & Evolution, 3(7), 1048–1056.

    Article  Google Scholar 

  • Quevedo, M., Svanbäck, R., & Eklöv, P. (2009). Intrapopulation niche partitioning in a generalist predator limits food web connectivity. Ecology, 90(8), 2263–2274.

    Article  Google Scholar 

  • Quinn, R. L., & Lepre, C. J. (2021). Contracting eastern African C4 grasslands during the extinction of Paranthropus boisei. Scientific Reports, 11(1), 1–10.

    Article  Google Scholar 

  • Roberts, P., Fernandes, R., Craig, O. E., Larsen, T., Lucquin, A., Swift, J., & Zech, J. (2018). Calling all archaeologists: Guidelines for terminology, methodology, data handling, and reporting when undertaking and reviewing stable isotope applications in archaeology. Rapid Communications in Mass Spectrometry, 32(5), 361–372.

    Article  Google Scholar 

  • Robinson, J. R., & Rowan, J. (2017). Holocene paleoenvironmental change in southeastern Africa (Makwe Rockshelter, Zambia): Implications for the spread of pastoralism. Quaternary Science Reviews, 156, 57–68.

    Article  Google Scholar 

  • Robinson, J. R., Rowan, J., Campisano, C. J., Wynn, J. G., & Reed, K. E. (2017). Late Pliocene environmental change during the transition from Australopithecus to Homo. Nature Ecology & Evolution, 1(6), 1–7.

    Article  Google Scholar 

  • Rossman, S., Ostrom, P. H., Gordon, F., & Zipkin, E. F. (2016). Beyond carbon and nitrogen: Guidelines for estimating three-dimensional isotopic niche space. Ecology and Evolution, 6(8), 2405–2413.

    Article  Google Scholar 

  • Scaffidi, B. K., Kamenov, G. D., Sharpe, A. E., & Krigbaum, J. (2021). Non-local enemies or local subjects of violence?: Using strontium (87Sr/86Sr) and lead (206Pb/204Pb, 207Pb/204Pb, 208Pb/204Pb) isobiographies to reconstruct geographic origins and early childhood mobility of decapitated male heads from the Majes Valley, Peru. Journal of Archaeological Method and Theory, 1-54.

  • Schoeninger, M. J. (1985). Trophic level effects on 15N/14N and 13C/12C ratios in bone collagen and strontium levels in bone mineral. Journal of Human Evolution, 14(5), 515–525.

    Article  Google Scholar 

  • Schoeninger, M. J. (1989). Reconstructing prehistoric human diet. In T. D. Price (Ed.), The chemistry of prehistoric human bone (pp. 38-67). Cambridge University Press Cambridge.

  • Schoeninger, M. J., & DeNiro, M. J. (1984). Nitrogen and carbon isotopic composition of bone collagen from marine and terrestrial animals. Geochimica et Cosmochimica acta, 48(4), 625–639.

    Article  Google Scholar 

  • Schoeninger, M. J., & Moore, K. (1992). Bone stable isotope studies in archaeology. Journal of World Prehistory, 6(2), 247–296.

    Article  Google Scholar 

  • Schoeninger, M. J., Reeser, H., & Hallin, K. (2003). Paleoenvironment of Australopithecus anamensis at Allia Bay, East Turkana, Kenya: Evidence from mammalian herbivore enamel stable isotopes. Journal of Anthropological Archaeology, 22(3), 200–207.

    Article  Google Scholar 

  • Sealy, J. C., van der Merwe, N. J., Sillen, A., Kruger, F. J., & Krueger, H. W. (1991). 87Sr/86Sr as a dietary indicator in modern and archaeological bone. Journal of Archaeological Science, 18(3), 399-416.

  • Sealy, J. C., Armstrong, R., & Schrire, C. (1995). Beyond lifetime averages: Tracing life histories through isotopic analysis of different calcified tissues from archaeological human skeletons. Antiquity, 69(263), 290.

    Article  Google Scholar 

  • Shackleton, N. J., Backman, J., Zimmerman, H. T., Kent, D. V., Hall, M. A., Roberts, D. G., Schnitker, D., Baldauf, J. G., Desprairies, A., Homrighausen, R., Huddlestun, P., Keene, J. B., Kaltenback, A. J., Krumsiek, K. A. O., Morton, A. C., Murray, J. W., & Westberg-Smith, J. (1984). Oxygen isotope calibration of the onset of ice-rafting and history of glaciation in the North Atlantic region. Nature, 307(5952), 620–623.

    Article  Google Scholar 

  • Sharma, S., Joachimski, M. M., Tobschall, H. J., Singh, I. B., Tewari, D. P., & Tewari, R. (2004). Oxygen isotopes of bovid teeth as archives of paleoclimatic variations in archaeological deposits of the Ganga plain, India. Quaternary Research, 62(1), 19–28.

    Article  Google Scholar 

  • Sponheimer, M., Alemseged, Z., Cerling, T. E., Grine, F. E., Kimbel, W. H., Leakey, M. G., Lee-Thorp, J. A., Manthi, F. K., Reed, K. E., Wood, B. A., & Wynn, J. G. (2013). Isotopic evidence of early hominin diets. Proceedings of the National Academy of Sciences, 110(26), 10513–10518.

    Article  Google Scholar 

  • Swanson, H. K., Lysy, M., Power, M., Stasko, A. D., Johnson, J. D., & Reist, J. D. (2015). A new probabilistic method for quantifying n-dimensional ecological niches and niche overlap. Ecology, 96(2), 318–324.

    Article  Google Scholar 

  • Ugan, A., & Coltrain, J. (2012). Stable isotopes, diet, and taphonomy: A look at using isotope-based dietary reconstructions to infer differential survivorship in zooarchaeological assemblages. Journal of Archaeological Science, 39(5), 1401–1411.

    Article  Google Scholar 

  • Van der Merwe, N. J., & Vogel, J. C. (1978). 13C content of human collagen as a measure of prehistoric diet in Woodland North America. Nature, 276(5690), 815-816.

  • Vogel, J. C., & Van Der Merwe, N. J. (1977). Isotopic evidence for early maize cultivation in New York State. American Antiquity, 238–242.

  • Ward, C. V., Plavcan, J. M., & Manthi, F. K. (2010). Anterior dental evolution in the Australopithecus anamensis–afarensis lineage. Philosophical Transactions of the Royal Society B: Biological Sciences, 365(1556), 3333–3344.

    Article  Google Scholar 

  • West, C. F., & France, C. A. (2015). Human and canid dietary relationships: Comparative stable isotope analysis from the Kodiak Archipelago, Alaska. Journal of Ethnobiology, 35(3), 519–535.

    Article  Google Scholar 

  • Wright, L. E. (2012). Immigration to Tikal, Guatemala: Evidence from stable strontium and oxygen isotopes. Journal of Anthropological Archaeology, 31(3), 334–352.

    Article  Google Scholar 

  • Wynn, J. G., Sponheimer, M., Kimbel, W. H., Alemseged, Z., Reed, K., Bedaso, Z. K., & Wilson, J. N. (2013). Diet of Australopithecus afarensis from the Pliocene Hadar formation, Ethiopia. Proceedings of the National Academy of Sciences, 110(26), 10495–10500.

    Article  Google Scholar 

  • Wynn, J. G., Alemseged, Z., Bobe, R., Grine, F. E., Negash, E. W., & Sponheimer, M. (2020). Isotopic evidence for the timing of the dietary shift toward C4 foods in eastern African Paranthropus. Proceedings of the National Academy of Sciences, 117(36), 21978–21984.

    Article  Google Scholar 

  • Yeakel, J. D., Bhat, U., Elliott Smith, E. A., & Newsome, S. D. (2016). Exploring the isotopic niche: Isotopic variance, physiological incorporation, and the temporal dynamics of foraging. Frontiers in Ecology and Evolution, 4, 1.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Joshua R. Robinson.

Ethics declarations

Competing Interests

The author declares no competing interests.

Additional information

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Supplementary Information

ESM 1

(XLSX 30 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Robinson, J.R. Investigating Isotopic Niche Space: Using rKIN for Stable Isotope Studies in Archaeology. J Archaeol Method Theory 29, 831–861 (2022). https://doi.org/10.1007/s10816-021-09541-7

Download citation

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10816-021-09541-7

Keywords

Navigation