Skip to main content
SpringerLink
Log in

Release and antimicrobial activity of levofloxacin from composite mats of poly(ɛ-caprolactone) and mesoporous silica nanoparticles fabricated by core–shell electrospinning

  • Original Paper
  • Published:
Journal of Materials Science Aims and scope Submit manuscript

Abstract

Nanofibrous materials have often been reported as carriers for clinical drugs but face the limitation of releasing the drugs in a burst fashion during use. The aim of this study is to produce composite nanofibrous mats with sustained release, using the broad spectrum antibiotic levofloxacin (LVF) as a model. Sustained release was achieved through two approaches, i.e. by firstly loading LVF into mesoporous silica nanoparticles (MSN) and then incorporating the MSN in the core regions of poly(ɛ-caprolactone) (PCL) nanofibres via core–shell electrospinning. Uniform PCL/LVF nanofibrous mats were also produced as controls. Loading of LVF into the MSN nanopores was confirmed by FTIR, BJH and BET measurements (100 mg LVF/g MSN). After electrospinning, electron microscopy revealed that the MSN were indeed confined in the core regions of the nanofibres. Drug release profiles showed that burst release was decreased from 59 % in the uniform PCL/LVF electrospun mats to 39 % in the core–shell PCL/LVF–MSN mats after 1 day in phosphate buffer at 37 °C, and gradual release in the latter was observed over the next 13 days. Antimicrobial assays showed that the composite electrospun mats were highly effective in killing Escherichia coli even after the mats had been incubated in a phosphate buffer for 14 days while the uniform PCL/LVF mats lost the ability after only 7 days. The results indicate that adsorption of the drug onto MSN and confining them in the core of nanofibres are effective ways of minimizing burst release and achieving sustained release of the drug.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

References

  1. Langer R (1998) Drug delivery and targeting. Nature 392(6679 Suppl):5–10

    Google Scholar 

  2. De Jong WH, Borm PJ (2008) Drug delivery and nanoparticles: applications and hazards. Int J Nanomed 3(2):133

    Article  Google Scholar 

  3. Jain K (2008) Drug delivery systems—an overview. In: Jain K (ed) Drug delivery systems. Humana Press, Totowa, pp 1–50

    Chapter  Google Scholar 

  4. Lavan DA, McGuire T, Langer R (2003) Small-scale systems for in vivo drug delivery. Nat Biotechnol 21(10):1184–1191

    Article  Google Scholar 

  5. Ranade VV, Cannon JB (2011) Drug delivery systems. CRC Press, Boca Raton

    Google Scholar 

  6. Ramakrishna S et al (2005) An introduction to electrospinning and nanofibers, vol 90. World Scientific, Singapore

    Book  Google Scholar 

  7. Wang X et al (2002) Electrospinning technology: a novel approach to sensor application. J Macromol Sci Part A 39(10):1251–1258

    Article  Google Scholar 

  8. Huang Z-M et al (2003) A review on polymer nanofibers by electrospinning and their applications in nanocomposites. Compos Sci Technol 63(15):2223–2253

    Article  Google Scholar 

  9. Kenawy E-R et al (2009) Processing of polymer nanofibers through electrospinning as drug delivery systems. Mater Chem Phys 113(1):296–302

    Article  Google Scholar 

  10. Sill TJ, von Recum HA (2008) Electrospinning: applications in drug delivery and tissue engineering. Biomaterials 29(13):1989–2006

    Article  Google Scholar 

  11. Zeng J et al (2003) Biodegradable electrospun fibers for drug delivery. J Control Release 92(3):227–231

    Article  Google Scholar 

  12. Kenawy E-R et al (2007) Controlled release of ketoprofen from electrospun poly (vinyl alcohol) nanofibers. Mater Sci Eng A 459(1):390–396

    Article  Google Scholar 

  13. He CL et al (2006) Coaxial electrospun poly (L-lactic acid) ultrafine fibers for sustained drug delivery. J Macromol Sci Part B 45(4):515–524

    Article  Google Scholar 

  14. Zong X et al (2002) Structure and process relationship of electrospun bioabsorbable nanofiber membranes. Polymer 43(16):4403–4412

    Article  Google Scholar 

  15. Xiaoqiang L et al (2009) Fabrication and properties of core-shell structure P (LLA-CL) nanofibers by coaxial electrospinning. J Appl Polym Sci 111(3):1564–1570

    Article  Google Scholar 

  16. Huang ZM et al (2006) Encapsulating drugs in biodegradable ultrafine fibers through co-axial electrospinning. J Biomed Mater Res Part A 77(1):169–179

    Article  Google Scholar 

  17. Song W et al (2013) Coaxial PCL/PVA electrospun nanofibers: osseointegration enhancer and controlled drug release device. Biofabrication 5(3):035006

    Article  Google Scholar 

  18. Yu D et al (2013) Electrospun biphasic drug release polyvinylpyrrolidone/ethyl cellulose core/sheath nanofibers. Acta Biomater 9(3):5665–5672

    Article  Google Scholar 

  19. Qian W et al (2014) Dual drug release electrospun core-shell nanofibers with tunable dose in the second phase. Int J Mol Sci 15(1):774–786

    Article  Google Scholar 

  20. Su Y et al (2011) Encapsulation and controlled release of heparin from electrospun poly (l-lactide-co-ε-caprolactone) nanofibers. J Biomater Sci Polym Ed 22(1–3):165–177

    Article  Google Scholar 

  21. Wang C et al (2010) Biodegradable core/shell fibers by coaxial electrospinning: processing, fiber characterization, and its application in sustained drug release. Macromolecules 43(15):6389–6397

    Article  Google Scholar 

  22. Meng ZX et al (2011) Fabrication, characterization and in vitro drug release behavior of electrospun PLGA/chitosan nanofibrous scaffold. Mater Chem Phys 125(3):606–611

    Article  Google Scholar 

  23. Li L et al (2011) Electrospun poly (ɛ-caprolactone)/silk fibroin core-sheath nanofibers and their potential applications in tissue engineering and drug release. Int J Biol Macromol 49(2):223–232

    Article  Google Scholar 

  24. Tarn D et al (2013) Mesoporous silica nanoparticle nanocarriers: biofunctionality and biocompatibility. Acc Chem Res 46(3):792–801

    Article  Google Scholar 

  25. Liu X (2013) Fabrication of heparinized mesoporous silica nanoparticles as multifunctional drug carriers. J Chem 2013:430459

    Google Scholar 

  26. Grumezescu AM et al (2013) Biocompatible magnetic hollow silica microspheres for drug delivery. Curr Org Chem 17(10):1029–1033

    Article  Google Scholar 

  27. Song B, Wu C, Chang J (2012) Dual drug release from electrospun poly(lactic-co-glycolic acid)/mesoporous silica nanoparticles composite mats with distinct release profiles. Acta Biomater 8(5):1901–1907

    Article  Google Scholar 

  28. Tsai C-H et al (2011) Surfactant-assisted controlled release of hydrophobic drugs using anionic surfactant templated mesoporous silica nanoparticles. Biomaterials 32(26):6234–6244

    Google Scholar 

  29. He Q, Shi J (2011) Mesoporous silica nanoparticle based nano drug delivery systems: synthesis, controlled drug release and delivery, pharmacokinetics and biocompatibility. J Mater Chem 21(16):5845–5855

    Article  Google Scholar 

  30. He Q et al (2009) Intracellular localization and cytotoxicity of spherical mesoporous silica nano- and microparticles. Small 5(23):2722–2729

    Article  Google Scholar 

  31. Yang P, Gai S, Lin J (2012) Functionalized mesoporous silica materials for controlled drug delivery. Chem Soc Rev 41(9):3679–3698

    Article  Google Scholar 

  32. Kortesuo P et al (2000) Silica xerogel as an implantable carrier for controlled drug delivery—evaluation of drug distribution and tissue effects after implantation. Biomaterials 21(2):193–198

    Article  Google Scholar 

  33. Slowing II et al (2008) Mesoporous silica nanoparticles as controlled release drug delivery and gene transfection carriers. Adv Drug Deliv Rev 60(11):1278–1288

    Article  Google Scholar 

  34. Douroumis D (2011) Encapsulation of water insoluble drugs in mesoporous silica nanoparticles using supercritical carbon dioxide. J Nanomed Nanotechnol 2:111

    Google Scholar 

  35. Levine MM (1984) Escherichia coli infections. In: Germanier R (ed) Bacterial vaccines. Academic Press, Inc., New York, pp 187–235

    Chapter  Google Scholar 

  36. Allen T (1997) Particle size measurement: volume 2: surface area and pore size determination, vol 2. Springer, New York

    Google Scholar 

  37. Zhang Y et al (2010) Spherical mesoporous silica nanoparticles for loading and release of the poorly water-soluble drug telmisartan. J Control Release 145(3):257–263

    Article  Google Scholar 

  38. Shi Y-T et al (2010) The size-controllable synthesis of nanometer-sized mesoporous silica in extremely dilute surfactant solution. Mater Chem Phys 120(1):193–198

    Article  Google Scholar 

  39. Middleton JC, Tipton AJ (2000) Synthetic biodegradable polymers as orthopedic devices. Biomaterials 21(23):2335–2346

    Article  Google Scholar 

  40. Nair LS, Laurencin CT (2007) Biodegradable polymers as biomaterials. Prog Polym Sci 32(8):762–798

    Article  Google Scholar 

  41. Li Z-Z et al (2004) Fabrication of porous hollow silica nanoparticles and their applications in drug release control. J Control Release 98(2):245–254

    Article  Google Scholar 

  42. Kanehata M, Ding B, Shiratori S (2007) Nanoporous ultra-high specific surface inorganic fibres. Nanotechnology 18(31):315602

    Article  Google Scholar 

  43. Park H et al (2012) Fabrication of levofloxacin-loaded nanofibrous scaffolds using coaxial electrospinning. J Pharm Investig 42:1–5

    Article  Google Scholar 

  44. Kim TG, Lee DS, Park TG (2007) Controlled protein release from electrospun biodegradable fiber mesh composed of poly (ɛ-caprolactone) and poly (ethylene oxide). Int J Pharm 338(1):276–283

    Article  Google Scholar 

  45. Pawlak A, Mucha M (2003) Thermogravimetric and FTIR studies of chitosan blends. Thermochim Acta 396(1):153–166

    Article  Google Scholar 

  46. Torres-Giner S et al (2012) Controlled delivery of gentamicin antibiotic from bioactive electrospun polylactide-based ultrathin fibers. Adv Eng Mater 14(4):B112–B122

    Article  Google Scholar 

  47. Nguyen TTT, Chung OH, Park JS (2011) Coaxial electrospun poly(lactic acid)/chitosan (core/shell) composite nanofibers and their antibacterial activity. Carbohydr Polym 86(4):1799–1806

    Article  Google Scholar 

  48. Lu F et al (2011) Effects of amphiphilic PCL–PEG–PCL copolymer addition on 5-fluorouracil release from biodegradable PCL films for stent application. Int J Pharm 419(1):77–84

    Article  Google Scholar 

  49. McDonald PF et al (2010) In vitro degradation and drug release from polymer blends based on poly (dl-lactide), poly (l-lactide-glycolide) and poly (ε-caprolactone). J Mater Sci 45(5):1284–1292. doi:10.1007/s10853-009-4080-9

    Article  Google Scholar 

Download references

Acknowledgements

This work has been supported by CSIRO’s Manufacturing flagship. We would also like to thank Chi Huynh for her help with TEM.

Author information

Authors and Affiliations

  1. School of Fashion and Textiles, College of Design and Social Context, RMIT University, 25 Dawson Street, Brunswick, VIC, 3056, Australia

    Javid Jalvandi, Max White & Rajiv Padhye

  2. CSIRO, Manufacturing Flagship, Bayview Ave, Clayton, VIC, 3168, Australia

    Javid Jalvandi, Yen Bach Truong, Yuan Gao & Ilias Louis Kyratzis

Authors
  1. Javid Jalvandi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Max White
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yen Bach Truong
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Yuan Gao
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Rajiv Padhye
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Ilias Louis Kyratzis
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Javid Jalvandi.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Jalvandi, J., White, M., Truong, Y.B. et al. Release and antimicrobial activity of levofloxacin from composite mats of poly(ɛ-caprolactone) and mesoporous silica nanoparticles fabricated by core–shell electrospinning. J Mater Sci 50, 7967–7974 (2015). https://doi.org/10.1007/s10853-015-9361-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10853-015-9361-x

Keywords

Search

Navigation