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Dual-acting biofunctionalised scaffolds for applications in regenerative medicine

  • Tissue Regeneration or Regeneration of Engineered Tissue?
  • Original Research
  • Published:
Journal of Materials Science: Materials in Medicine Aims and scope Submit manuscript

Abstract

Off the shelf scaffolds for replacing ultra-small diameter vascular grafts are valuable for reconstruction of diseased or damaged vessels. The limitations for such grafts include optimal handling with ready availability of varied lengths of grafts, graft patency with the ability to replace the function of active cellular mechanisms and adequate mechanical properties to maintain physicochemical function. We used a well-established, solvent casting method for potential tissue replacement scaffold fabrication with incorporated bioactive molecules, which we have previously explored to confer haemocompatibility. These grafts were tested in-vivo within the abdominal aorta of 10 Wistar rats and the patency was clinically and echographically evaluated. Haemocompatibility and endothelialisation were assessed on explants. Biofunctionalised scaffolds were also grafted subcutaneously and intraperitoneally to evaluate integration, inflammation and angiogenesis reactions. The potential wider applications of this dual acting scaffold were evaluated for its interactions with human dermal fibroblasts as well as bronchial epithelial cells. Physicochemical property evaluation of the functionalised grafts has clarified the mechanical strength and permeability. This study confirmed the microsurgical suturability of tubular grafts and graft patency of functionalized scaffolds. The study demonstrated the potential of a dual acting biofunctionalised scaffold’s use for a wide range of tissue engineering applications where micro-porous, yet impermeable scaffolds are needed.

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Acknowledgements

Authors would like to thank Arnold Darbyshire at UCL for synthesising and providing with polyurethane for this study and Nathalie Poitevin and Jean Luc Vignes at Ecole de Chirurgie, Paris for their support in the microsurgical laboratory. ME is sponsored by University of Alexandria, Egypt and JH is sponsored by Globe Microsystems Ltd UK.

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    Authors and Affiliations

    1. Division of Surgery and Interventional Science, University College London, London, UK

      Camilo Chaves, Chuanyu Gao, Jerome Hunckler, Moustafa Elsawy & Achala de Mel

    2. Université Pierre et Marie Curie, 4 Place Jussieu, Paris, 75005, France

      Camilo Chaves

    3. Ecole de Chirurgie, AGEPS, AP-HP, 7 Rue du Fer À Moulin, Paris, 75005, France

      Camilo Chaves & Josette Legagneux

    4. University College London Hospitals, London, UK

      Moustafa Elsawy

    5. Institut Cochin—INSERM U1016, 27 rue du fbg Saint Jacques, Paris, 75014, France

      Gilles Renault

    6. Hôpital St Antoine, Service de Chirurgie Orthopédique & Traumatologique, Unité Chirurgie Réparatrice & Chirurgie de la Main, Paris, F-75571, France

      Alain Charles Masquelet

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    1. Camilo Chaves
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    Correspondence to Achala de Mel.

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    Camilo Chaves and Chuanyu Gao contributed equally to the study.

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    Chaves, C., Gao, C., Hunckler, J. et al. Dual-acting biofunctionalised scaffolds for applications in regenerative medicine. J Mater Sci: Mater Med 28, 32 (2017). https://doi.org/10.1007/s10856-017-5849-z

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