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Scaffolds of PCL combined to bioglass: synthesis, characterization and biological performance

  • Tissue Engineering Constructs and Cell Substrates
  • Original Research
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Abstract

Biomaterials may be useful in filling lost bone portions in order to restore balance and improve bone regeneration. The objective of this study was to produce polycaprolactone (PCL) membranes combined with two types of bioglass (Sol–Gel and melt-quenched) and determine their physical and biological properties. Membranes were produced through electrospinning. This study presented three experimental groups: pure PCL membranes, PCL-Melt-Bioglass and PCL-Sol-gel-Bioglass. Membranes were characterized using Scanning Electron Microscopy, Fourier Transform Infrared Spectrophotometry (FTIR), Energy-Dispersive Spectroscopy and Zeta Potential. The following in vitro tests were performed: MTT assay, alkaline phosphatase activity, total protein content and mineralization nodules. Twenty-four male rats were used to observe biological performance through radiographic, fracture energy, histological and histomorphometric analyses. The physical and chemical analysis results showed success in manufacturing bioactive membranes which significantly enhanced cell viability and osteoblast differentiation. The new formed bone from the in vivo experiment was similar to that observed in the control group. In conclusion, the electrospinning enabled preparing PCL membranes with bioglass incorporated into the structure and onto the surface of PCL fibers. The microstructure of the PCL membranes was influenced by the bioglass production method. Both bioglasses seem to be promising biomaterials to improve bone tissue regeneration when incorporated into PCL.

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Acknowledgements

We thank the Fundação de Amparo à Pesquisa do Estado de São Paulo—FAPESP, for the scholarship process no. 17/04389-0. We thank the Coordination for the Improvement of Higher Education (CAPES—Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Brasil)—Finance Code 001, in São José dos Campos, Brazil.

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Authors and Affiliations

  1. Department of Dental Materials and Prosthodontics, Institute of Science and Technology of Sao Jose dos Campos, São Paulo State University (UNESP), Av. Engenheiro José Longo, 777, Jd São Dimas, São José dos Campos, SP, 12245000, Brazil

    Gabriela Fernandes da Fonseca, Renata Falchete do Prado & Alexandre Luiz Souto Borges

  2. Department of Bioscience and Oral Diagnosis, Institute of Science and Technology of Sao Jose dos Campos, São Paulo State University (UNESP), Av. Engenheiro José Longo, 777, Jd São Dimas, São José dos Campos, SP, 12245000, Brazil

    Sarah de Oliveira Marco Avelino, Daphne de Camargo Reis Mello & Luana Marotta Reis de Vasconcellos

  3. Technological Institute of Aeronautics (ITA), Praça Marechal Eduardo Gomes, 50 Vila das Acácias, São José dos Campos, SP, 12228-900, Brazil

    Tiago Moreira Bastos Campos

  4. Bioceramics Laboratory (BIOCERAM), Institute of Science and Technology, Federal University of São Paulo—UNIFESP, Av. Cesare Monsueto Giulio Lattes1201—Eugênio de Melo, São José dos Campos, SP, 12247-014, Brazil

    Eliandra de Sousa Trichês

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  1. Gabriela Fernandes da Fonseca
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  2. Sarah de Oliveira Marco Avelino
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  3. Daphne de Camargo Reis Mello
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  4. Renata Falchete do Prado
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  5. Tiago Moreira Bastos Campos
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  7. Eliandra de Sousa Trichês
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  8. Alexandre Luiz Souto Borges
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Correspondence to Renata Falchete do Prado.

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da Fonseca, G.F., Avelino, S.d.O.M., Mello, D.d.C.R. et al. Scaffolds of PCL combined to bioglass: synthesis, characterization and biological performance. J Mater Sci: Mater Med 31, 41 (2020). https://doi.org/10.1007/s10856-020-06382-w

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  • DOI: https://doi.org/10.1007/s10856-020-06382-w

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