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Metabolomics Analysis of the Effect of Hydrogen-Rich Water on Myocardial Ischemia-Reperfusion Injury in Rats

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Abstract

To investigate the effect of hydrogen-rich water on myocardial tissue metabolism in a myocardial ischemia-reperfusion injury (MIRI) rat model. Twelve rats were randomly divided into a hydrogen-rich water group and a control group of size 6 each. After the heart was removed, it was fixed in the Langendorff device, and the heart was perfused with 37 °C perfusion solution pre-balanced with oxygen. The control group was perfused with Kreb’s-Ringers (K-R) solution, and the hydrogen-rich water group was perfused with K-R solution + hydrogen-rich water. Liquid Chromatograph Mass Spectrometer (LC-MS) analysis platform was used for metabolomics research. Principle component analysis (PCA), partial least squares discriminant analysis (PLS-DA), orthogonal partial least squares discriminant analysis (OPLS-DA), Variable importance in projection (VIP) value of OPLS-DA model (threshold value ≥1) were employed with independent sample T Test (p < 0.05) to find differentially expressed metabolites, and screen for differential metabolic pathways. VIP (OPLS-DA) analysis was performed with T test, and the metabolites of the control group and the hydrogen-rich water group were significantly different, and the glycerophospholipid metabolism was screened. Seven myocardial ischemia-reperfusion injury (MIRI)-related signaling pathways were identified, including glycerophospholipid metabolism, glycosylphosphatidylinositol (GPI) anchored biosynthesis, and purine metabolism, as well as 10 biomarkers such as phosphatidylcholine, phosphatidylethanolamine and phosphatidylserine. Hydrogen-rich water regulates the metabolic imbalance that could change MIRI myocardial tissue metabolism, and alleviate ischemia-reperfusion injury in isolated hearts of rats through multiple signaling pathways.

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Data Availability

All data generated or analyzed during the present study are included in this published article.

Abbreviations

AMI:

Acute myocardial infarction

GPI:

Glycosylphosphatidylinositol

LC-MS:

Liquid Chromatograph Mass Spectrometer

MIRI:

Myocardial ischemia-reperfusion injury

NAD+ :

Nicotinamide adenine dinucleotide

OPLS-DA:

Orthogonal partial least squares discriminant analysis

PLS-DA:

Partial least squares discriminant analysis

PCA:

Principle component analysis

TCA:

Tricarboxylic acid

TIC:

Total ion current

VIP:

Variable importance in projection

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Acknowledgements

The authors are grateful for the support provided by the Affiliated Hospital of Hebei University of China. The authors are grateful for the experimental hydrogen-rich water provided by Professor Zhilin Li from the College of Chemistry, Hebei University (patent number ZL102557227B).

Funding

This research was financially supported by the Medical Science Research Key Project Foundation of Hebei Province (No. 20130369).

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Author notes

  1. Liangtong Li and Tongtong Liu contributed equally to this work.

Authors and Affiliations

  1. Medical College, Hebei University, Baoding, 071000, China

    Liangtong Li, Li Liu, Zhe Zhang, Shaochun Li & Yujuan Zhou

  2. Affiliated Hospital of Hebei University, Baoding, 071000, China

    Tongtong Liu & Fulin Liu

  3. Department of Cardiology, Baoding First Center Hospital, Baoding, 071000, China

    Zhiling Zhang

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  1. Liangtong Li
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Contributions

Liangtong Li, Tongtong Liu, Li Liu and Zhe Zhang performed the LC-MS analyses. Liangtong Li and Tongtong Liu collected the data and performed the statistical analyses. Shaochun Li and Zhiling Zhang participated in sample collection and the signaling pathway analysis. Yujuan Zhou and Fulin Liu designed the study. Liangtong Li and Tongtong Liu wrote the manuscript. All authors read and approved the final manuscript.

Corresponding authors

Correspondence to Yujuan Zhou or Fulin Liu.

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Conflict of interests

The authors declare that they have no competing interests.

Ethics approval and consent to participate

The present study was performed at the Central Laboratory of Affiliated Hospital of Hebei University. All animal experiments were approved by the Animal Ethical and Welfare Committee of Hebei University (Baoding, China, Approval No. 2017010) and performed in accordance with the Guidelines for the Care and Use of Laboratory Animals of Hebei University.

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Liangtong Li and Tongtong Liu, should be regarded as co-first authors.

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Li, L., Liu, T., Liu, L. et al. Metabolomics Analysis of the Effect of Hydrogen-Rich Water on Myocardial Ischemia-Reperfusion Injury in Rats. J Bioenerg Biomembr 52, 257–268 (2020). https://doi.org/10.1007/s10863-020-09835-7

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