Abstract
Males of the desert beetle Parastizopus armaticeps (Pér.) (Coleoptera: Tenebrionidae) exhibit a characteristic calling behavior that attracts females by raising the tip of the abdomen, exposing the aedeagus, and remaining in this posture for a few seconds while emitting a pheromone. We collected the pheromone by holding a solid phase microextraction fiber (100 μm polydimethylsiloxane) close to the aedeagus for 5 s and analyzed the volatiles collected by gas chromatography/mass spectrometry. The volatiles consisted of 3-methylphenol (52%), ethyl-1,4-benzoquinone (48%), and 3-ethylphenol (2%). The pheromone originated from the aedeagal glands. In the gland reservoirs, these compounds (2.1%) were mixed with ethyl, isopropyl, and propyl esters of fatty acids (24.2%), and a mixture of hydrocarbons (69.1%). The mean amount of volatiles extracted from gland reservoirs was 0.92 ± 0.83 μg. Chemo-orientation experiments with a servosphere show that females responded only to the ternary volatile mixture. Females stopped walking, elevated the front parts of their bodies with erected antennae, turned slowly on their own axis, and walked upwind toward the odor source. Single components or binary mixtures did not elicit responses from females. Males did not respond to the pheromone. Evolutionary aspects of this pheromone system are discussed.
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References
Batschelet, E. 1981. Circular Statistics in Biology. Academic, London.
Blum, M. S. 1981. Chemical Defenses of Arthropods. Academic, New York.
Brits, J. A. 1982. The anatomy, histology and physiology of the internal adult male reproductive system of Parastizopus armaticeps Péringuey (Coleoptera: Tenebrionidae). J. Entomol. Soc. South. Afr. 45:239–260.
Brown, W. V., Doyen, J. T., Moore, B. P., and Lawrence, J. F. 1992. Chemical composition and taxonomic significance of defensive secretions of some Australian Tenebrionidae (Coleoptera). J. Aust. Entomol. Soc. 31:79–89.
Carlson, D. A., Bernier, U. R., and Sutton, B. D. 1998. Elution patterns from capillary GC for methyl-branched alkanes. J. Chem. Ecol 24:1845–1865.
Geiselhardt, S., Ockenfels, P., and Peschke, K. 2008. 1-Tridecene—male-produced sex pheromone of the tenebrionid beetle Parastizopus transgariepinus. Naturwissenschaften 95:247–251.
Geiselhardt, S., Szepat, T., Rasa, O. A. E., and Peschke, K. 2006. Defensive secretion components of the host Parastizopus armaticeps as kairomones for the cleptoparasite Eremostibes opacus. J. Chem. Ecol. 32:767–778.
Keville, R., and Kannowski, P. B. 1975. Sexual excitation by pheromones of the confused flour beetle. J. Insect Physiol. 21:81–84.
Koch, C. 1963. The Tenebrionidae of Southern Africa XXIX. Luebbertia plana gen. et spec. Nov., with a dichotomic analysis of Stizopina (Opatrini). Scientific paper Namib Des. Res. Station 18.
Leal, W. S. 1997. Evolution of sex pheromone communication in plant-feeding scarab beetles, pp, pp. 505–513, in R. T. Cardé, and A. K. Minks (eds.). Insect Pheromone Research: New DirectionsChapman and Hall, New York.
Loconti, J. D., and Roth, L. M. 1953. Composition of the odorous secretion of Tribolium castaneum. Ann. Entomol. Soc. Am 46:281–289.
Nelson, D. R., and Sukkestad, D. R. 1970. Normal and branched aliphatic hydrocarbons from eggs of the tobacco hornworm. Biochemistry 9:4601–4611.
Nelson, D. R., Sukkestad, D. R., and Zaylskie, R. G. 1972. Mass spectra of methyl-branched hydrocarbons from eggs of the tobacco hornworm. J. Lipid Res 13:413–421.
O’Brien, W. J., Browman, H. I., and Evans, B. I. 1990. Search strategies of foraging animals. Am. Sci. 78:152–160.
Otálora-Luna, F., Perret, J.-L., and Guerin, P. M. 2004. Appetence behaviours of the triatomine bug Rhodnius prolixus on a servosphere in response to the host metabolites carbon dioxide and ammonia. J. Comp. Physiol. A 190:847–854.
Peschke, K. 1983. Defensive and pheromonal secretion of the tergal gland of Aleochara curtula II. Release and inhibition of male copulatory behavior. J. Chem. Ecol 9:13–31.
Peschke, K., and Metzler, M. 1982. Defensive and pheromonal secretion of the tergal gland of Aleochara curtula. II. The chemical composition. J. Chem. Ecol. 8:773–778.
Pomonis, J. G., Nelson, D. R., and Fatland, C. L. 1980. Insect hydrocarbons. 2. Mass spectra of dimethylalkanes and the effect of the number of methylene units between groups on fragmentation. J. Chem. Ecol 6:965–972.
Rasa, O. A. E. 1990. Evidence for subsociality and division of labor in a desert tenebrionid beetle Parastizopus armaticeps Peringuey. Naturwissenschaften 77:591–592.
Rasa, O. A. E., Bisch, S., and Teichner, T. 1998. Female mate choice in a subsocial beetle: male phenotype correlates with helping potential and offspring survival. Anim. Behav. 56:1213–1220.
Reinecke, A., Ruther, J., and Hilker, M. 2002. The scent of food and defence: green leaf volatiles and toluquinone as sex attractant mediate mate finding in the European cockchafer Melolontha melolontha. Ecol. Lett. 5:257–263.
Ruther, J., Reinecke, A., Tolasch, T., and Hilker, M. 2001. Make love not war: a common arthropod defence compound as sex pheromone in the forest cockchafer Melolontha hippocastani. Oecologia 128:44–47.
Ruther, J., Reinecke, A., Tolasch, T., and Hilker, M. 2002. Phenol—another cockchafer attractant shared by Melolontha hippocastani Fabr. and M. melolontha L. Z. Naturforsch 57:910–913.
Scribe, P., Guezennec, J., Dagaut, J., Pepe, C., and Saliot, A. 1988. Identification of the position and the stereochemistry of the double bond in monounsaturated fatty acid methyl esters by gas chromatography/mass spectrometry of dimethyl disulfide derivatives. Anal. Chem 60:928–931.
Tobin, T. R., and Bell, W. J. 1986. Chemo-orientation of male Trogoderma variabile (Coleoptera, Dermestidae) in a simulated corridor of female sex pheromone. J. Comp. Physiol A 158:729–739.
Tschinkel, W. R. 1969. Phenols and quinones from the defensive secretions of the tenebrionid beetle, Zophobas rugipes. J. Insect Physiol. 15:191–200.
Tschinkel, W. R. 1975a. A comparative study of the chemical defensive system of tenebrionid beetles—chemistry of the secretions. J. Comp. Physiol 21:753–783.
Tschinkel, W. R. 1975b. A comparative study of the chemical defensive system of tenebrionid beetles—III. Morphology of the glands. J. Morphol. 145:355–370.
Walter, F. 1993. Identification of the sex pheromone of an ant, Formica lugubris (Hymenoptera, Formicidae). Naturwissenschaften 80:30–34.
Willis, M. A., and Avondet, J. L. 2005. Odor-modulated orientation in walking male cockroaches Periplaneta americana, and the effect of odor plumes of different structure. J. Exp. Biol. 208:721–735.
Acknowledgment
We thank Stefanie Geiselhardt and two anonymous reviewers for helpful comments on the manuscript. This work was supported by the Deutsche Forschungsgemeinschaft, Bonn (Pe 231/13-1,2).
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Geiselhardt, S., Jakobschy, D., Ockenfels, P. et al. A Sex Pheromone in the Desert Tenebrionid Beetle Parastizopus armaticeps . J Chem Ecol 34, 1065–1071 (2008). https://doi.org/10.1007/s10886-008-9488-1
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DOI: https://doi.org/10.1007/s10886-008-9488-1