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Interaction of the La(III)–Morin Complex with Human Serum Albumin

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Abstract

Lanthanum (La) is one of the most reactive rare earth elements, whose carbonate had been approved by the FDA for marketing as a treatment drug for hyperphosphatemia. In this paper, a complex of La with the natural active compound morin was synthesized and identified. The interaction between synthesized complex (LaMO) and human serum albumin (HSA) was studied by multiple spectroscopic methods. It was found that LaMO has an efficient interaction with HSA through hydrogen bonds and van der Waals forces, with formation of the LaMO–HSA complex in its ground state. The reaction led to quenching of HSA’s fluorescence and the quenching follows a static quenching mechanism. The binding constants, reference state enthalpy change (ΔH θ), Gibbs energy change (ΔG θ) and entropy change (ΔS θ) were calculated at four different temperatures. Fluorescence probe techniques were used to identify the binding location. The results showed that LaMO competes with warfarin for Sudlow’s site I in subdomain IIA of HSA, an acknowledged site marker. Meanwhile, circular dichroism spectrum measurements revealed changes of HSA’s secondary structure in the presence of LaMO, which implies that LaMO is potentially bioactive. The study involves research about the pharmacologic mechanism of rare earth metal coordination compounds and can provide basic data for their safety evaluation.

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Acknowledgments

The authors gratefully acknowledge the fund support of the Science and Technology Research Program of the Education Department, Hubei, China (Grants No. Q20134303 and Q20144304) and the Scientific Research Project of Jingchu University of Technology (Grant No. ZR201302; ZR201108). The work is also supported by Hubei Key Laboratory of Drug Synthesis and Optimization (Grant No. OPP2014YB01).

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  1. Hubei Key Laboratory of Drug Synthesis and Optimization, Jingchu University of Technology, Jingmen, Hubei, 448000, People’s Republic of China

    E. Liu & Hua-xin Zhang

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  1. E. Liu
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  2. Hua-xin Zhang
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Correspondence to Hua-xin Zhang.

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Liu, E., Zhang, Hx. Interaction of the La(III)–Morin Complex with Human Serum Albumin. J Solution Chem 43, 1402–1413 (2014). https://doi.org/10.1007/s10953-014-0210-3

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