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Design and in vivo evaluation of alginate-based pH-sensing electrospun wound dressing containing anthocyanins

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Abstract

In this study, a pH-sensing electrospun mat containing purple cabbage (PC) (Brassica oleracea) anthocyanins was developed for wound dressing application. The pH-sensing electrospun mat was prepared from sodium alginate (Alg) and poly (vinyl alcohol) (PVA) via electrospinning. PC anthocyanins were embedded into the electrospun mat with the addition of PC extract into the Alg/PVA electrospun solution. The characterization studies were conducted by scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), water contact angle (WCA) and thickness measurements. The halochromic behavior of the pH-sensing Alg/PVA/PC electrospun mat was elucidated via UV–Vis spectrophotometric analyses and color measurements obtained from quantitative color data. In vivo performance of the pH-sensing mat was evaluated by animal studies. The electrospun mat was successfully used to monitor pH changes of an open wound. In addition, wound healing efficacy of the electrospun mat was compared with a commercial wound dressing. The results revealed that a wound dressing benefiting from halochromic properties of the anthocyanins besides wound healing efficacy of Alg was successfully developed. The pH-sensing Alg/PVA/PC electrospun mat has a usage potential for wound dressing applications especially for monitoring wound status on real-time during healing period.

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Funding

This study was financially supported by The Scientific and Technological Research Council of Turkey (TUBITAK) and was assigned project number 116M540.

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Authors and Affiliations

  1. Graduate School of Natural and Applied Sciences, Bursa Uludag University, 16059, Görükle, Bursa, Turkey

    Ayben Pakolpakçıl

  2. Department of Chemistry, Faculty of Arts and Science, Bursa Uludag University, 16059, Görükle, Bursa, Turkey

    Bilgen Osman & Elif Tümay Özer

  3. Department of Pharmacology, Faculty of Medicine, Bursa Uludag University, 16059, Görükle, Bursa, Turkey

    Gökhan Göktalay

  4. Department of Food Engineering, Faculty of Agriculture, Bursa Uludag University, 16059, Görükle, Bursa, Turkey

    Yasemin Şahan

  5. Department of Textile Engineering, Faculty of Engineering, Bursa Uludag University, 16059, Görükle, Bursa, Turkey

    Behçet Becerir & Esra Karaca

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  1. Ayben Pakolpakçıl
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  2. Bilgen Osman
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Contributions

Ayben Pakolpakçıl; Investigation, Formal analysis, Methodology, Bilgen Osman; Conceptualization, Methodology, Writing—original draft, Gökhan Göktalay; Investigation, Formal analysis, Methodology, Writing—original draft, Elif Tümay Özer; Investigation, Formal analysis, Methodology, Visualization, Yasemin Şahan; Formal analysis, Methodology, Behçet Becerir; Formal analysis, Methodology, Esra Karaca; Conceptualization, Resources, Funding acquisition, Project administration, Writing—original draft, Writing—review & editing.

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Correspondence to Esra Karaca.

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Pakolpakçıl, A., Osman, B., Göktalay, G. et al. Design and in vivo evaluation of alginate-based pH-sensing electrospun wound dressing containing anthocyanins. J Polym Res 28, 50 (2021). https://doi.org/10.1007/s10965-020-02400-1

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