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Effects of Ganoderma Lucidum shell-broken spore on oxidative stress of the rabbit urinary bladder using an in vivo model of ischemia/reperfusion

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Abstract

Oxidative stress plays an important role in specific disease pathophysiology and the aging process. In the history of human kind, many herbs were utilized for disease prevention and anti-aging treatment. However, there are few direct evidences provided by modern laboratory technology. The current study was designed to evaluate Ganoderma Lucidum’s (GL) ability to reduce the damage from in vivo ischemia/reperfusion (I/R) using a rabbit model of I/R that has been effectively utilized to prove the effects of drugs and supplements to reduce oxidative stress. Urinary bladder dysfunction secondary to benign prostatic hyperplasia (BPH) is a major affliction of aging men. One of the major etiologies of obstructive bladder dysfunction (OBD) is oxidative stress induced by I/R. Pharmaceutical studies and clinical research have proven that GL is useful in helping to prevent certain types of pathology and also helpful in prolonging human life in part by acting as an antioxidant. Using an in vivo model of I/R, we have investigated the ability of GL to protect bladder function from oxidative damage mediated by I/R. Our studies demonstrated that ischemia followed by reperfusion resulted in a significant decrease in bladder compliance and decreases in the contractile responses to a variety of forms of contractile stimulation. Pretreatment of rabbits with Ganoderma Lucidum prior to subjecting the rabbits to I/R completely inhibited the negative effects of I/R on both the compliance and contractile responses. These results demonstrate that Ganoderma provides excellent protection of bladder function following I/R (oxidative stress).

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Acknowledgements

These studies were based on work supported in part by Wellstrong Biotech Company, Beijing Tong Ren Tang Chinese medicine Co. LTD, the Office of Research and Development Department of the Veterans Affairs, and by the Capital Region Medical Research Foundation.

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Authors and Affiliations

  1. Stratton VA Medical Center, Albany, NY, 12208, USA

    Robert M. Levin, Catherine Schuler & Robert E. Leggett

  2. Albany College of Pharmacy and Health Science, Albany, NY, USA

    Robert M. Levin

  3. Beijing Tong Ren Tang Chinese Medicine Co., LTD., Beijing, China

    Li Xia, Wu Wei & Alpha D.-Y. Lin

  4. The Central-Clinic Hospital, Taipei, Taiwan

    Alpha D.-Y. Lin

  5. Urology Department, National Yang-Ming University, Taipei, Taiwan

    Alpha D.-Y. Lin

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  1. Robert M. Levin
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  2. Li Xia
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  3. Wu Wei
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  4. Catherine Schuler
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  5. Robert E. Leggett
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  6. Alpha D.-Y. Lin
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Correspondence to Robert M. Levin.

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Levin, R.M., Xia, L., Wei, W. et al. Effects of Ganoderma Lucidum shell-broken spore on oxidative stress of the rabbit urinary bladder using an in vivo model of ischemia/reperfusion. Mol Cell Biochem 435, 25–35 (2017). https://doi.org/10.1007/s11010-017-3053-6

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