Abstract
The present study was undertaken to explore the role of total flavones of Camellia (TFC) on cerebral injury following subarachnoid hemorrhage (SAH) in rats. We showed that the increase of malondialdehyde (MDA) level in brain tissues, leakages of neuron-specifc enolase (NSE) and lactate dehydrogenase (LDH) from brain tissues to serum at 48 h after SAH were significantly blocked by TFC treatment. Besides, TFC treatment could reduce brain edema and the Bax/Bcl-2 ratio in hippocampal tissues at mRNA and protein levels at 48 h after SAH. In addition, and the reduction of neurological scores at 7d after SAH were significantly inhibited by TFC treatment. We next sought to demonstrate the role of TFC on cognitive rehabilitation and the tau phosphorylation in hippocampal tissues at 30d after SAH. Not surprisingly, cognitive dysfunction and the upregulation of tau phosphorylation at Ser262 (p-tau-Ser262) in hippocampal tissues were markedly reduced by TFC treatment. These findings suggested that TFC has protective effect on SAH-induced EBI and subsequent cognitive dysfunction, which may be related to downregulating the Bax/Bcl-2-related apoptosis pathway and inhibition of tau phosphorylation.
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Abbreviations
- TFC:
-
total flavones of Camellia
- SAH:
-
Subarachnoid hemorrha
- EBI:
-
early brain injur
- MDA:
-
malondialdehyde
- NSE:
-
neuron-specifc enolase
- LDH:
-
lactate dehydrogenase
- CJ:
-
Camellia japonica L.
- ECJ:
-
Camellia japonica L. flower
- MWM:
-
Morris water maze
- RT-PCR:
-
Reverse Transcription Polymerase chain reaction
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Funding
This study was supported by Grants for Scientific Research of BSKY (No. XJ201612) from Anhui Medical University and from Natural Science Foundation of Hefei Technology College (No. 201914KJA020).
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Wen JY: Participated in research design and writing of the manuscript; Lu WZ: Participated in experiments and data analysis.
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All animal experiments were reviewed and approved by the Ethics Review Committee of Anhui Medical University, which comply with the Guide for the Care and Use of laboratory Animals published by the US National Institutes of Health (NIH publication no. 85 − 23, revised 2011).
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Lu, W., Wen, J. Neuroprotective roles of total flavones of Camellia on early brain injury andcognitive dysfunction following subarachnoid hemorrhage in rats. Metab Brain Dis 35, 775–783 (2020). https://doi.org/10.1007/s11011-020-00567-6
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DOI: https://doi.org/10.1007/s11011-020-00567-6