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Quantitative trait loci of barley malting quality trait components in the Stellar/01Ab8219 mapping population

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Abstract

Malting barley is of high economic and scientific importance. Determining barley grains that are suitable for malting involves measuring malting quality, which is an expensive and complex process. In order to decrease the cost of phenotyping and accelerate the process of developing superior malting barley cultivars, markers for marker-assisted breeding are needed. In this study, we identified quantitative trait loci (QTLs) for malting traits in a Stellar/01Ab8219 F6:8 recombinant inbred line population grown at Aberdeen and Tetonia, Idaho, USA in 2009 and 2010. We identified QTLs associated with malt extract (ME), wort protein, soluble/total protein (S/T), diastatic power (DP), alpha-amylase, beta-glucan (BG) and free amino nitrogen (FAN) at a logarithm of odds score ≥2.5 using a high-density genetic map produced by merging Diversity Arrays Technology markers with the current single nucleotide polymorphism map. Novel QTLs were identified for DP and FAN on chromosome 5H, S/T on 6H, and BG and ME on 7H. Dissection of the genetic regions associated with malting traits suggests the involvement of multiple molecular pathways. The resulting molecular markers may prove useful for barley improvement.

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Acknowledgments

We thank and recognize the efforts of Chris Evans, Kathy Satterfield, Irene Shackelford and Robert Campbell for their management of the Idaho field projects and barley samples. We thank MillerCoors staff Kathy Adams and Krista Huettig for malting analysis of specific RILs and Christopher Martens from USDA-ARS Cereal Crops Research Unit (CCRU) at Madison, WI for his assistance with malting data. In addition we thank the staff of the University of Idaho Research and Extension Experiment Stations in Tetonia and Aberdeen, Idaho for field management. This work was funded by the USDA Barley for Rural Development Grant, Idaho Barley Commission and American Malting Barley Association (AMBA). The authors recognize and appreciate the support of Idaho barley growers and Ms. Kelly Olson.

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Authors and Affiliations

  1. USDA ARS, 1691 S. 2700 W., Aberdeen, ID, 83210, USA

    Emir Islamovic, Gongshe Hu & Kathy E. Klos

  2. General Mills, Inc., 150 Research Campus Dr., Kannapolis, NC, 28081, USA

    Ryan H. Brown & Eric W. Jackson

  3. Limagrain Cereal Seeds, 4846 East 450 North, Lafayette, IN, 47905, USA

    Donald E. Obert

  4. Department of Plant, Soil, and Entomological Sciences, University of Idaho, 1776 Science Center Dr. Suite 205, Idaho Falls, ID, 83660-9637, USA

    Juliet M. Marshall

  5. Department of Plant and Wildlife Sciences, Brigham Young University, Provo, UT, 84602, USA

    Eric N. Jellen & Peter J. Maughan

  6. USDA ARS Cereal Crop Research Unit, 502 Walnut St., Madison, WI, 53726, USA

    Allen D. Budde & Mark Schmitt

  7. Diversity Arrays Technology, 1 Wilf Crane Cr., Yarralumla, ACT, 2600, Australia

    Andrzej Kilian

  8. USDA ARS, 1605 Albrecht Blvd., Fargo, ND, 58105, USA

    Shiaoman Chao

  9. USDA ARS, 800 Buchanan St., Albany, CA, 94710, USA

    Gerard R. Lazo

  10. MillerCoors, 7 N 400 W, Burley, ID, 83318, USA

    Robert Brunick II

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  1. Emir Islamovic
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  2. Donald E. Obert
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  3. Allen D. Budde
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  4. Mark Schmitt
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  6. Andrzej Kilian
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  8. Gerard R. Lazo
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  9. Juliet M. Marshall
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  11. Peter J. Maughan
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  12. Gongshe Hu
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  13. Kathy E. Klos
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  14. Ryan H. Brown
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  15. Eric W. Jackson
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Correspondence to Donald E. Obert.

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Islamovic, E., Obert, D.E., Budde, A.D. et al. Quantitative trait loci of barley malting quality trait components in the Stellar/01Ab8219 mapping population. Mol Breeding 34, 59–73 (2014). https://doi.org/10.1007/s11032-014-0017-3

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