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Modulation of structural carbohydrates, phenol compounds and lignin content in Eucalyptus urophylla cuttings grown under boron, copper and zinc induced-deficiency

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Abstract

Micronutrients participate in various plant metabolic pathways, including the synthesis of carbohydrates, lignin, and phenols, which are necessary for plant growth and defense against pests and pathogens. In this study, we evaluated the effects of nutrient solutions deplete in B, Cu, or Zn on the nutrition, growth, and production of biochemical compounds in Eucalyptus urophylla cuttings. The experiment was carried out in a greenhouse for 90 days, with clonal cuttings of E. urophylla at four months of age. The treatments evaluated were complete fertilization (CF) and individual omissions of B, Cu, and Zn in the nutrient solution. The omission of Cu and Zn increased the concentration of carbohydrates in the leaves by 10% and reduced the rate of CO2 assimilation (A) by more than 30%. Cu omission decreased the lignification process compared to complete fertilization. Flavonoid production in plants with complete fertilization was 45–75% higher than that in plants maintained under B, Cu, or Zn deficiencies. Collectively, these results suggest that micronutrient deficiencies may reduce plant productivity as well as plant resistance to pests and pathogens.

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Funding

This study received support from São Paulo Research Foundation—FAPESP (Process: 2018/07294–3), The Brazilian National Council for Scientific and Technological Development (CNPq) and Silviculture and Management Thematic Program (PTSM-IPEF).

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Authors and Affiliations

  1. Luiz de Queiroz” College of Agriculture (ESALQ), University of São Paulo, 11 Ave. Pádua Dias, Piracicaba, SP, 13418-900, Brazil

    Liamara Santos Masullo, Vitória Duarte Derisso, Gislene Roberta Manarim, Patrícia Andressa de Ávila, Antonio Leite Florentino, Claudio Lima de Aguiar & José Leonardo de Moraes Gonçalves

  2. Institute of Forest Research and Study (IPEF), Via Comendador Pedro Morganti, Piracicaba, SP, 13415-000, Brazil

    Alexandre de Vicente Ferraz

  3. Geplant Forest Technology, St Dr João Sampaio, Piracicaba, SP, 13416-383, Brazil

    José Henrique Tertulino Rocha

  4. Center for Nuclear Energy in Agriculture (CENA), University of São Paulo, 303 Ave. Centenário, Piracicaba, SP, 13416-000, Brazil

    José Lavres

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  1. Liamara Santos Masullo
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  2. Vitória Duarte Derisso
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  3. Gislene Roberta Manarim
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  10. José Leonardo de Moraes Gonçalves
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Contributions

LSM, AVF and JLMG designed this research. LSM, VDD, GRM, PAA and ALF performed this research. LSM, VDD and JHTR analyzed the data. JLMG, AVF, CLA, JL and JHTR revised this draft by rewriting, discussion and commenting. All authors contributed in the same way to the preparation of all the parts of this manuscript.

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Correspondence to Liamara Santos Masullo.

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Masullo, L.S., Derisso, V.D., Manarim, G.R. et al. Modulation of structural carbohydrates, phenol compounds and lignin content in Eucalyptus urophylla cuttings grown under boron, copper and zinc induced-deficiency. New Forests 53, 337–352 (2022). https://doi.org/10.1007/s11056-021-09859-w

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