In rats with the kindling syndrome induced by i.p. injections of pentylenetetrazole (PTZ; 35 mg/kg daily, for three weeks), the averaged latency of seizures induced by a test injection of PTZ (35 mg/kg) was significantly greater (by 42.2%, P < 0.05 vs. control) after a combination of pioglitazone administration (100 mg/kg, i.p.) and transcranial DC stimulation (tDCS, 300 μA, 10 min, cathode on the skull surface) oriented toward the cerebellar cortex. Also, combined using of tDCS and pioglitazone prevented the occurrence of generalized tonico-clonic seizure attacks in 8 out of 10 rats of the respective group (P < 0.05), reduced the seizure severity by 31.3% on average (P < 0.05), and made shorter ictal discharges (by 45.0%; P < 0.05).
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L. S. Godlevsky and M. P. Pervak, “Peroxisomal proliferator-activated γ-receptors: participation in the anti-seizure effects of transcranial DC stimulation of the cerebellum,” Neurophysiology, 51, No. 1, 25–28 (2019), https://doi.org/10.1007/s11062-019-09786-9.
D. M. Abdallah, “Anticonvulsant potential of the peroxisome proliferator-activated receptor γ agonist pioglitazone in pentylenetetrazole-induced acute seizures and kindling in mice,” Brain Res., 1351, 246–253 (2010). https://doi.org/10.1016/j.brainres.2010.06.034.
R. A. Mohazab, M. Javadi-Peydar, B. Delfan, and A. R. Dehpour, “Possible involvement of PPAR-gamma receptor and nitric oxide pathway in the anticonvulsant effect of acute pioglitazone on pentylenetetrazole-induced seizures in mice,” Epilepsy Res., 101, Nos. 1–2, 28–35 (2012), https://doi.org/10.1016/j.eplepsyres.2012.02.015.
T. Y. Hung, F. L. Chu, D. C. Wu, et al., “The protective role of peroxisome proliferator-activated receptor-gamma in seizure and neuronal excitotoxicity,” Mol. Neurobiol., 56, No. 8, 5497–5506 (2019), https://doi.org/10.1007/s12035-018-1457-2.
H. A. Hussein, A. Moghimi, and A. Roohbakhsh, “Anticonvulsant and ameliorative effects of pioglitazone on cognitive deficits, inflammation and apoptosis in the hippocampus of rat pups exposed to febrile seizure,” Iran. J. Basic Med. Sci., 22, No. 3, 267–276 (2019), https://doi.org/10.22038/ijbms.2019.35056.8339.
L. S. Godlevsky, T. N. Muratova, N. V. Kresyun, et al., “Anxiolytic and antidepressive effects of electric stimulation of the paleocerebellar cortex in pentylenetetrazole kindled rats,” Acta Neurobiol. Exp. (Wars.), 74, No. 4, 456–464 (2014).
G. Paxinos and C. Watson, The Rat Brain in Stereotaxic Coordinates, Academic Press, Sydney (1998).
G. N. Kryzhanovsky, A. A. Shandra, L. S. Godlevsky, and A. M. Mazarati, “Antiepileptic system,” Usp. Fiziol. Nauk (Arch. Physiol. Sci.) [Russian], 23, No. 3, 12–32 (1992).
M. L. Streng and E. Krook-Magnuson, “The cerebellum and epilepsy,” Epilepsy Behav., 121, Pt. B, 106909 (2020), https://doi.org/10.1016/j.yebeh.2020.106909.
R. Ciccocioppo and M. Ubaldi, “Nuclear peroxisome proliferator activated receptor-gamma (PPARγ) as a therapeutic target to treat neurodegeneration and dependence elicited by drugs of abuse,” Neural Regen. Res., 16, No. 5, 984–985 (2021), https://doi.org/10.4103/1673-5374.297072
T. Vanaveski, S. Molchanova, D. D. Pham, et al., “PGC-1α signaling increases GABA(A) receptor subunit α2 expression, GABAergic neurotransmission and anxiety-like behavior in mice,” Front. Mol. Neurosci., 14, 588230 (2021), https://doi.org/10.3389/fnmol.2021.588230
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Godlevsky, L.S., Poshyvak, O.B., Pervak, M.P. et al. Effects of a Combination of Transcranial DC Cerebellar Stimulation and Pioglitazone Administration on Pentylenetetrazole-Induced Seizures in Kindled Rats. Neurophysiology 53, 88–92 (2022). https://doi.org/10.1007/s11062-022-09919-7
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DOI: https://doi.org/10.1007/s11062-022-09919-7