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Identification and characterization of AtCASP, a plant transmembrane Golgi matrix protein

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Abstract

Golgins are a family of coiled-coil proteins that are associated with the Golgi apparatus. They are necessary for tethering events in membrane fusion and may act as structural support for Golgi cisternae. Here we report on the identification of an Arabidopsis golgin which is a homologue of CASP, a known transmembrane mammalian and yeast golgin. Similar to its homologues, the plant CASP contains a long N-terminal coiled-coil region protruding into the cytosol and a C-terminal transmembrane domain with amino acid residues which are highly conserved across species. Through fluorescent protein tagging experiments, we show that plant CASP localizes at the plant Golgi apparatus and that the C-terminus of this protein is sufficient for its localization, as has been shown for its mammalian counterpart. In addition, we demonstrate that the plant CASP is able to localize at the mammalian Golgi apparatus. However, mutagenesis of a conserved tyrosine in the transmembrane domain revealed that it is necessary for ER export and Golgi localization of the Arabidopsis CASP in mammalian cells, but is not required for its correct localization in plant cells. These data suggest that mammalian and plant cells have different mechanisms for concentrating CASP in the Golgi apparatus.

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Authors and Affiliations

  1. Department of Biology, University of Saskatchewan, 112 Science Place, Saskatoon, SK, S7N 5E2, Canada

    Luciana Renna, Sally L. Hanton, Giovanni Stefano, Lauren Bortolotti & Federica Brandizzi

  2. Department of Veterinary Sciences, University of Saskatchewan, 52 Campus Drive, Saskatoon, SK, S7N 5B, Canada

    Vikram Misra

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  1. Luciana Renna
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  2. Sally L. Hanton
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  3. Giovanni Stefano
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  4. Lauren Bortolotti
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  5. Vikram Misra
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  6. Federica Brandizzi
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Correspondence to Federica Brandizzi.

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†These authors have contributed equally to the work

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Renna, L., Hanton, S.L., Stefano, G. et al. Identification and characterization of AtCASP, a plant transmembrane Golgi matrix protein. Plant Mol Biol 58, 109–122 (2005). https://doi.org/10.1007/s11103-005-4618-4

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