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Evolution of MIR159/319 genes in Brassica campestris and their function in pollen development

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MIR159/319 have conserved evolution and diversified function after WGT in Brassica campestris, both of them can lead pollen vitality and germination abnormality, Bra-MIR319c also can function in flower development.

Abstract

MiR159 and miR319 are extensively studied highly conserved microRNAs which play roles in vegetative development, reproduction, and hormone regulation. In this study, the effects of whole-genome triplication (WGT) on the evolution of the MIR159/319 family and the functional diversification of the genes were comprehensively investigated in Brassica campestris. We identified 11 MIR159/319 genes in B. campestris, which produced five mature sequences. After analyzing the precursor sequences and phylogenetic tree, we found that Bra-MIR159/319 have evolutionary conservatism. Furthermore, Bra-MIR159/319 show functional diversification after WGT, as indicated by their expression patterns and the cis-element in their promoter. GUS signal showed that Bra-MIR159a and Bra-MIR319c can be expressed in anther but in different development stages. In B. campestris, overexpressed MIR159a and MIR319c contribute to late anther development and promote pollen abortion. Moreover, Bra-MIR319c can partially assume the function of MIR319a in flower development.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (No. 31772311).

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Authors and Affiliations

  1. Laboratory of Cell and Molecular Biology, Institute of Vegetable Science, Zhejiang University, Hangzhou, 310058, China

    Ziwei Hu, Xiuping Shen, Xun Xiang & Jiashu Cao

  2. Key Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Ministry of Agriculture, Hangzhou, 310058, China

    Xun Xiang & Jiashu Cao

  3. Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology, Hangzhou, 310058, China

    Xun Xiang & Jiashu Cao

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  1. Ziwei Hu
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  2. Xiuping Shen
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  4. Jiashu Cao
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Ziwei Hu designed all the experiments. Xiuping She and Xun Xiang took part in this work and the manuscript modification. Jiaoshu Cao is the corresponding author.

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Correspondence to Jiashu Cao.

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Hu, Z., Shen, X., Xiang, X. et al. Evolution of MIR159/319 genes in Brassica campestris and their function in pollen development. Plant Mol Biol 101, 537–550 (2019). https://doi.org/10.1007/s11103-019-00920-z

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  • DOI: https://doi.org/10.1007/s11103-019-00920-z

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