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Kill or cure? The interaction between endophytic Paenibacillus and Serratia strains and the host plant is shaped by plant growth conditions

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Abstract

Aims

Verticillium wilt is difficult to suppress, and causes severe yield losses in a broad range of crops. Five Serratia and five Paenibacillus endophytic isolates showing antagonistic properties against fungal pathogens were compared for their plant growth-promoting (PGP) potential under different plant growth conditions with the objective of evaluating the PGP of endophytic strains in different ad planta systems.

Methods

Preselected isolates were applied to the surface-sterilized seeds of oilseed rape and cauliflower using bio-priming. The isolates’ PGP effect and root colonization capacities were compared under gnotobiotic conditions. One strain from each genus was selected and tested for its PGP qualities in sterile and non-sterile soil.

Results

Serratia treatment resulted in different levels of PGP, while Paenibacillus strains damaged roots under gnotobiotic conditions. P. polymyxa Sb3-1 did not have a significant effect on plant growth in non-sterile soil; however it did promote plant growth in the sterile soil. S. plymuthica 3RP8 and P. polymyxa Sb3-1 were selected for further testing of their biocontrol effect under field conditions.

Conclusions

The choice of growth environments in the investigation of plant-bacterium interaction is crucial. Non-sterile soil is suggested as the ideal medium for use in studying the PGP effect.

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Acknowledgments

The authors would like to thank Timothy Mark (Graz) for English revision and discussion. This project was funded by the European Union in frame of FP7-KBBE-2013-7-single-stage (BIOCOMES; No. 612713) and by the Austrian Research Promotion Agency (FFG; No. 836466). The authors gratefully acknowledge support from NAWI Graz. We thank Christin Zachow (Graz) for her help regarding experimental questions and Anastasia Bragina (Graz) for help regarding statistical issues.

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Authors and Affiliations

  1. Institute of Environmental Biotechnology, Graz University of Technology, Petersgasse 12, 8010, Graz, Austria

    Daria Rybakova, Maria Schmuck, Ute Wetzlinger, Octavian Murgu, Henry Müller & Gabriele Berg

  2. Departamento de Agronomía, University of Córdoba, Campus Universitario de Rabanales, Edificio C4, 14071, Córdoba, Spain

    Angela Varo-Suarez

Authors
  1. Daria Rybakova
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  2. Maria Schmuck
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  3. Ute Wetzlinger
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  6. Henry Müller
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  7. Gabriele Berg
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Correspondence to Gabriele Berg.

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Responsible Editor: Birgit Mitter.

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Rybakova, D., Schmuck, M., Wetzlinger, U. et al. Kill or cure? The interaction between endophytic Paenibacillus and Serratia strains and the host plant is shaped by plant growth conditions. Plant Soil 405, 65–79 (2016). https://doi.org/10.1007/s11104-015-2572-8

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  • DOI: https://doi.org/10.1007/s11104-015-2572-8

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