Abstract
Animal husbandry is believed to predate farming of crops, and remains a core component of most agricultural systems. Historic breeding strategies were based largely on visual observation, crossing animals that were perceived to display enhanced merit. Advances in sequencing capacity coupled with reduced costs have allowed genomic selection tools to deliver significant contribution to breeding regimes. The application of genome editors to make specific changes to livestock genomes has the potential to deliver additional benefits.
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References
Burkard C, Lillico SG, Reid E, Jackson B, Mileham AJ, Ait-Ali T, Whitelaw CB, Archibald AL (2017) Precision engineering for PRRSV resistance in pigs: macrophages from genome edited pigs lacking CD163 SRCR1 domain are fully resistant to both PRRSV genotypes while maintaining biological function. PLoS Pathog 13(2):e1006206. https://doi.org/10.1371/journal.ppat.1006206
Burkard C, Opriessnig T, Mileham AJ, Stadejek T, Ait-Ali T, Lillico SG, Whitelaw CBA, Archibald AL (2018) Pigs lacking the scavenger receptor cysteine-rich domain 5 of CD163 are resistant to porcine reproductive and respiratory syndrome virus 1 infection. J Virol 92(16):e00415–e00418. https://doi.org/10.1128/jvi.00415-18
Cooper CA, Doran TJ, Challagulla A, Tizard MLV, Jenkins KA (2018) Innovative approaches to genome editing in avian species. J Anim Sci Biotechnol 9:15. https://doi.org/10.1186/s40104-018-0231-7
DiGiusto DL, Cannon PM, Holmes MC, Li L, Rao A, Wang J, Lee G, Gregory PD, Kim KA, Hayward SB, Meyer K, Exline C, Lopez E, Henley J, Gonzalez N, Bedell V, Stan R, Zaia JA (2016) Preclinical development and qualification of ZFN-mediated CCR1 disruption in human hematopoietic stem/progenitor cells. Mol Ther Methods Clin Dev 3:16067. https://doi.org/10.1038/mtm.2016.67
Lillico SG, Proudfoot C, King TJ, Tan W, Zhang L, Mardjuki R, Paschon DE, Rebar EJ, Urnov FD, Mileham AJ, McLaren DG, Whitelaw CB (2016) Mammalian interspecies substitution of immune modulatory alleles by genome editing. Sci Rep 6:21645. https://doi.org/10.1038/srep21645
McGrew MJ, Sherman A, Ellard FM, Lillico SG, Gilhooley HJ, Kingsman AJ, Mitrophanous KA, Sang H (2004) Efficient production of germline transgenic chickens using lentiviral vectors. EMBO Rep 5:728–733. https://doi.org/10.1038/sj.embor.7400171
Niemann H, Lucas-Hahn A (2012) Somatic cell nuclear transfer cloning: practical applications and current legislation. Reprod Domest Anim 47(Suppl 5):2–10. https://doi.org/10.1111/j.1439-0531.2012.02121.x
Oatley JM (2017) Recent advances for spermatogonial stem cell transplantation in livestock. Reprod Fertil Dev 30:44–49. https://doi.org/10.1071/RD17418
Park KE, Kaucher AV, Powell A, Waqas MS, Sandmaier SE, Oatley MJ, Park CH, Tibary A, Donovan DM, Blomberg LA, Lillico SG, Whitelaw CB, Mileham A, Telugu BP, Oatley JM (2017) Generation of germline ablated male pigs by CRISPR/Cas9 editing of the NANOS2 gene. Sci Rep 7:40176. https://doi.org/10.1038/srep40176
Proudfoot C, Carlson DF, Huddart R, Long CR, Pryor JH, King TJ, Lillico SG, Mileham AJ, McLaren DG, Whitelaw CB, Fahrenkrug SC (2015) Genome edited sheep and cattle. Transgenic Res 24:147–153. https://doi.org/10.1007/s11248-014-9832-x
Qasim W, Zhan H, Samarasinghe S, Adams S, Amrolia P, Stafford S, Butler K, Rivat C, Wright G, Somana K, Ghorashian S, Pinner D, Ahsan G, Gilmour K, Lucchini G, Inglott S, Mifsud W, Chiesa R, Peggs KS, Chan L, Farzeneh F, Thrasher AJ, Vora A, Pule M, Veys P (2017) Molecular remission of infant B-ALL after infusion of universal TALEN gene-edited CAR T cells. Sci Transl Med. https://doi.org/10.1126/scitranslmed.aaj2013
Tan W, Carlson DF, Lancto CA, Garbe JR, Webster DA, Hackett PB, Fahrenkrug SC (2013) Efficient nonmeiotic allele introgression in livestock using custom endonucleases. Proc Natl Acad Sci USA 110:16526–16531. https://doi.org/10.1073/pnas.1310478110
Wargelius A, Leininger S, Skaftnesmo KO, Kleppe L, Andersson E, Taranger GL, Schulz RW, Edvardsen RB (2016) Dnd knockout ablates germ cells and demonstrates germ cell independent sex differentiation in Atlantic salmon. Sci Rep 6:21284. https://doi.org/10.1038/srep21284
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Disclaimer: The opinions expressed and arguments employed in this paper are the sole responsibility of the author and do not necessarily reflect those of the OECD or of the governments of its Member countries.
This paper was given at the OECD Conference on Genome Editing: Applications in Agriculture—Implications for Health, Environment and Regulation which took place at the OECD on 28 and 29 June, and which was sponsored by the OECD Co‐operative Research Programme: Biological Resource Management for Sustainable Agricultural Systems whose financial support made it possible for the author to participate in the conference.
