Abstract
The chicken is an exemplar of efficient intensive animal agriculture and provides two valuable food products, chicken meat and eggs. Only aquaculture is better, by efficiency, but poultry is still top, by mass of animal protein produced as food in the global context. However this efficiency and intensive production comes with a number of challenges. Though the genetics of selective breeding have led to dramatic improvements in yield, efficiency and product quality, traits that relate to disease and welfare outcomes have not been so tractable. These two issues are major impacts to the industry in terms of production and in terms of public perception. Both transgenic technology and genome editing have clear potential for impact in these two important areas. The reproductive biology of birds requires techniques very specific to birds to achieve heritable (germline) edited traits. These are quite involved and, even though they are now well-defined and reliable, there is room for improvement and advances can be expected in the future. Currently the key targets for this technology are modifying chicken genes involved in virus-receptor interactions and cellular response involved in infection. For the egg industry the technology is being applied to the issue of sex-selection for layer hens (and the removal of males), removal of allergens from egg white and the tailoring of eggs system to enhance the yield of influenza vaccine doses. Regulation and trading of the animals generated, and resulting food products, will significantly impact the value and future development of genome editing for poultry.
References
Bedell VM, Wang Y, Campbell JM, Poshusta TL, Starker CG, Krug RG, Tan W, Penheiter SG, Ma AC, Leung AY, Fahrenkrug SC, Carlson DF, Voytas DF, Clark KJ, Essner JJ, Ekker SC (2012) In vivo genome editing using a high efficiency TALEN system. Nature 491:114–118
Burkard C, Opriessnig T, Mileham AJ, Stadejek T, Ait-Ali T, Lillico SG, Whitelaw CBA, Archibald AL (2018) Pigs lacking the scavenger receptor cysteine-rich domain 5 of CD163 are resistant to porcine reproductive and respiratory syndrome virus 1 infection. J Virol 92:e00415-18. https://doi.org/10.1128/JVI.00415-18
Cho SW, Kim S, Kim JM, Kim JS (2013) Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease. Nat Biotechnol 31:230–232
Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini LA, Zhang F (2013) Multiplex genome engineering using CRISPR/Cas systems. Science 339:819–823
Cooper CA, Challagulla A, Jenkins KA, Wise TG, O’Neil TE, Morris KR, Tizard ML, Doran TJ (2017) Generation of gene edited birds in one generation using sperm transfection assisted gene editing (STAGE). Transgenic Res 26:331–347. https://doi.org/10.1007/s11248-016-0003-0
Dhanapala P, Withanage-Dona D, Tang ML, Doran T, Suphioglu C (2017) Hypoallergenic variant of the major egg white allergen Gal d 1 produced by disruption of cysteine bridges. Nutrients 9:171–182. https://doi.org/10.3390/nu9020171
Dimitrov L, Pedersen D, Ching KH, Yi H, Collarini EJ, Izquierdo S, van de Lavoir MC, Leighton PA (2016) Germline gene editing in chickens by efficient CRISPR-mediated homologous recombination in primordial germ cells. PLoS ONE 11:e0154303. https://doi.org/10.1371/journal.pone.0154303
Doran TJ, Cooper CA, Jenkins KA, Tizard ML (2016) Advances in genetic engineering of the avian genome: “realising the promise”. Transgenic Res 25:307–319. https://doi.org/10.1007/s11248-016-9926-8
Guan X, Zhang Y, Yu M, Ren C, Gao Y, Yun B, Liu Y, Wang Y, Qi X, Liu C, Cui H, Zhang Y, Gao L, Li K, Pan Q, Zhang B, Wang X, Gao Y (2017) Residues 28 to 39 of the extracellular loop 1 of chicken Na+/H+ exchanger type I mediate cell binding and entry of subgroup J avian leukosis virus. J Virol 92(1):e01627-17. https://doi.org/10.1128/JVI.01627-17
International Chicken Genome Sequencing Consortium (2004) Sequence and comparative analysis of the chicken genome provide unique perspectives on vertebrate evolution. Nature 432:695–716
Mali P, Yang L, Esvelt KM, Aach J, Guell M, DiCarlo JE, Norville JE, Church GM (2013) RNA-guided human genome engineering via Cas9. Science 339:823–826
Oishi I, Yoshii K, Miyahara D, Kagami H, Tagami T (2016) Targeted mutagenesis in chicken using CRISPR/Cas9 system. Sci Rep 6:23980. https://doi.org/10.1038/srep23980
Park TS, Lee HJ, Kim KH, Kim JS, Han JY (2014) Targeted gene knockout in chickens mediated by TALENs. PNAS 111:12716–12721
Tallentire CW, Leinonen I, Kyriazakis I (2016) Breeding for efficiency in the broiler chicken: a review. Agron Sustain Develop 33:66–82. https://doi.org/10.1007/s13593-016-0398-2
Tanaka K, Wada T, Koga O, Nishio Y, Hertelendy F (1994) Chick production by in vitro fertilization of the fowl ovum. J Reprod Fertil 100:447–449
Taylor L, Carlson DF, Nandi S, Sherman A, Fahrenkrug SC, McGrew MJ (2017) Efficient TALEN-mediated gene targeting of chicken primordial germ cells. Development 144:928–934. https://doi.org/10.1242/dev.145367
Tizard M, Hallerman E, Fahrenkrug S, Newell-McGloughlin M, Gibson J, de Loos F, Wagner S, Laible G, Han JY, D’Occhio M, Kelly L, Lowenthal J, Gobius K, Silva P, Cooper C, Doran T (2016) Strategies to enable the adoption of animal biotechnology to sustainably improve global food safety and security. Transgenic Res 25:575–595. https://doi.org/10.1007/s11248-016-9965-1
Tyack SG, Jenkins KA, O’Neil TE, Wise TG, Morris KR, Bruce MP, McLeod S, Wade AJ, McKay J, Moore RJ, Schat KA, Lowenthal JW, Doran TJ (2013) A new method for producing transgenic birds via direct in vivo transfection of primordial germ cells. Transgenic Res 22:1257–1264
van de Lavoir MC, Diamond JH, Leighton PA, Mather-Love C, Heyer BS, Bradshaw R, Kerchner A, Hooi LT, Gessaro TM, Swanberg SE, Delany ME, Etches RJ (2006) Germline transmission of genetically modified primordial germ cells. Nature 441:766–769
Warren WC, Hillier LW, Tomlinson C, Minx P, Kremitzki M, Graves T, Markovic C, Bouk N, Pruitt KD, Thibaud-Nissen F, Schneider V, Mansour TA, Brown CT, Zimin A, Hawken R, Abrahamsen M, Pyrkosz AB, Morisson M, Fillon V, Vignal A, Chow W, Howe K, Fulton JE, Miller MM, Lovell P, Mello CV, Wirthlin M, Mason AS, Kuo R, Burt DW, Dodgson JB, Cheng HH (2017) A new chicken genome assembly provides insight into avian genome structure. G3 (Bethesda)(7):109–117. https://doi.org/10.1534/g3.116.035923
Woodcock ME, Idoko-Akoh, McGrew MJ (2017) Gene editing in birds takes flight. Mamm Genome 28(7–8):315–323. https://doi.org/10.1007/s00335-017-9701-z
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Tizard, M.L., Jenkins, K.A., Cooper, C.A. et al. Potential benefits of gene editing for the future of poultry farming. Transgenic Res 28 (Suppl 2), 87–92 (2019). https://doi.org/10.1007/s11248-019-00139-0
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DOI: https://doi.org/10.1007/s11248-019-00139-0