Skip to main content

Advertisement

SpringerLink
Log in

Detection of antibodies against Brucella abortus, Leptospira spp., and Apicomplexa protozoa in water buffaloes in the Northeast of Argentina

  • Regular Articles
  • Published:
Tropical Animal Health and Production Aims and scope Submit manuscript

Abstract

Water buffalo industry has become a profitable activity worldwide, including the Northeast of Argentina (NEA). However, research on diseases affecting this species is scarce. The aim of the present study was to detect antibodies against Brucella abortus, Leptospira spp., Neospora caninum, Toxoplasma gondii, and Sarcocystis spp. in 500 water buffalo cows from five ranches (100 animals each) in the NEA. Serum samples were tested for B. abortus by fluorescence polarization assay, Leptospira spp. by microagglutination test, and N. caninum, T. gondii, and Sarcocystis spp. by indirect fluorescent antibody tests. Overall, the proportion of seropositive animals was 6.4, 22.2, 42.2, 25.4, and 50.8 % for brucellosis, leptospirosis, neosporosis, toxoplasmosis, and sarcocystosis, respectively. The proportion of seropositive animals for all diseases was statistically different among herds (p < 0.05). Statistical differences were also detected among age groups for brucellosis and neosporosis (p < 0.05). The detection of specific antibodies to B. abortus, Leptospira spp., and several Apicomplexa protozoans in water buffaloes in the NEA is reported in this study.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

References

  • Adesiyun, A.A., Hull-Jackson, C., Clarke, N., Whittington, C., Seepersadsingh, N., 2009. Leptospirosis in water buffalo (Bubalus bubalis) in Trinidad, Veterinarsky Arhiv, 79, 77–86.

    Google Scholar 

  • Borriello, G., Capparelli, R., Bianco, M., Fenizia, D., Alfano, F., Capuano, F., Ercolini, D., Parisi, A., Roperto, S., Iannelli, D., 2006. Genetic resistance to Brucella abortus in water buffalo (Bubalus bubalis), Infection and Immunity, 74, 2115–2120.

    Article  PubMed  CAS  Google Scholar 

  • Campero, C.M., Pérez, A., Moore, D.P., Crudeli, G., Benitez, D., Draghi, M.G., Cano, D., Konrad, J.L., Odeón, A.C., 2007. Occurrence of antibodies against Neospora caninum in water buffaloes (Bubalus bubalis) on four ranches in Corrientes province, Argentina, Veterinary Parasitology, 150, 155–158.

    Article  CAS  Google Scholar 

  • Capparelli, R., Alfano, F., Amoroso, M.G., Borriello, G., Fenizia, D., Bianco, A., Roperto, S., Roperto, F., Iannelli, D., 2007. Protective effect of the Nramp1 BB genotype against Brucella abortus in the water Buffalo (Bubalus bubalis), Infection and Immunity, 75, 988–996.

    Article  PubMed  CAS  Google Scholar 

  • Chen, X., Zuo, Y., Rosenthal, B.M., He, Y., Cui, L., Yang, Z., 2011. Sarcocystis sinensis is an ultrastructurally distinct parasite of water buffalo that can cause foodborne illness but cannot complete its life-cycle in human beings, Veterinary Parasitology, 178, 35–39.

    Article  PubMed  Google Scholar 

  • Chryssafidis, A.L., Soares, R.M., Rodrigues, A.A., Carvalho, N.A., Gennari, S.M., 2011. Evidence of congenital transmission of Neospora caninum in naturally infected water buffalo (Bubalus bubalis) fetus from Brazil, Parasitology Research, 108, 741–743.

    Article  PubMed  Google Scholar 

  • Crudeli, G.A., Patiño, M.E. 2011. Produção de Búfalos de Leite. Cap: 1, Origen do Búfalo e Principais Raças. Editora FEPAF, pp. 40.

  • Di Rienzo J.A., Casanoves F., Balzarini M.G., Gonzalez L., Tablada M., Robledo C.W. Info Stat versión 2011. Grupo InfoStat, FCA, Universidad Nacional de Córdoba, Argentina.

    Google Scholar 

  • Draghi, M.G., Brihuega, B., Benítez, D., Sala, J.M., Biotti, G.M., Pereyra, M., Homse, A., Guariniello, L., 2011. Brote de leptospirosis en terneros en recría en la provincia de Corrientes, Argentina, Revista Argentina de Microbiología, 43, 42–44.

    Google Scholar 

  • Dubey, J.P., Speer, C.A., Fayer, R., 1989. Sarcocystosis of Animals and Man (CRC, Inc., Florida).

    Google Scholar 

  • Dubey, J.P., Romand, S., Hilali, M., Kwok, O.C., Thuliez, P., 1998. Seroprevalence of antibodies to Neospora caninum and Toxoplasma gondii in water buffaloes (Bubalus bubalis) from Egypt, International Journal for Parasitology, 28, 527–529.

    Article  PubMed  CAS  Google Scholar 

  • Dubey, J.P., Hill, D.E., Jones, J.L., Hightower, A.W., Kirkland, E., Roberts, J.M., Marcet, P.L., Lehmann, T., Vianna, M.C.B., Miska, K., Sreekumar, C., Kwok, O.C.H., Shen, S.K., Gamble, H.R., 2005. Prevalence of viable Toxoplasma gondii in beef, chicken, and pork from retail meat stores in the United States: risk assessment to consumers, The Journal of Parasitology, 91, 1082–1093.

    Article  PubMed  CAS  Google Scholar 

  • El-Dakhly, K.M., El-Nesr, K.A., El-Nahass, El.S., Hirata, A., Sakai, H., Yanai, T., 2011. Prevalence and distribution patterns of Sarcocystis spp. in buffaloes in Beni-Suef, Egypt. Tropical Animal Health and Production, 43, 1549–1554.

  • Felt, S.A., Wasfy, M.O., El-Tras, W.F., Samir, A., Rahaman, B.A., Boshra, M., Parker, T.M., Hatem, M.E., El-Bassiouny, A.A., Murray, C.K., Pimentel, G., 2011. Cross-species surveillance of Leptospira in domestic and peri-domestic animals in Mahalla City, Gharbeya Governorate, Egypt. The American Journal of Tropical Medicine and Hygiene, 84, 420–425.

    Article  PubMed  Google Scholar 

  • Food and Agriculture Organization, FAO. 2005. Buffalo production and research. Rome.

  • Fujii, T.U., Kasai, N., Nishi, S.M., Dubey, J.P., Gennari, S.M., 2001. Seroprevalence of Neospora caninum in female water buffaloes (Bubalus bubalis) from the southeastern region of Brazil, Veterinary Parasitology, 99, 331–334.

    Article  PubMed  CAS  Google Scholar 

  • Galiero, G., Martucciello, A., Astarita, S., Iovane, G., Pagnini, U., Fusco, G., Guarino, A., 2006. Isolation of Brucella abortus strain RB51 from two buffalo fetuses, The Veterinary Record, 159, 563–564.

    Article  PubMed  CAS  Google Scholar 

  • Gennari, S.M., Rodrigues, A.A.R., Viana, R.B., Cardoso, E.C., 2005. Occurrence of anti-Neospora caninum antibodies in water buffaloes (Bubalus bubalis) from the Northern region of Brazil, Veterinary Parasitology, 134, 169–171.

    Article  PubMed  Google Scholar 

  • Hamidinejat, H., Ghorbanpour, M., Nabavi, L., Haji, Hajikolaie MR, Razi Jalali MH, 2010. Seroprevalence of Toxoplasma gondii in water buffaloes (Bubalus bubalis) in South-West of Iran. Tropical Biomedicine, 27, 275–279.

  • Huong, L.T., 1999. Prevalence of Sarcocystis spp. in water buffaloes in Vietnam, Veterinary Parasitology, 86, 33–39.

    Article  PubMed  CAS  Google Scholar 

  • Jehle, C., Dinkel, A., Sander, A., Morent, M., Romig, T., Luc, P.V., De, T.V., Thai, V.V., Mackenstedt, U., 2009. Diagnosis of Sarcocystis spp. in cattle (Bos taurus) and water buffalo (Bubalus bubalis) in Northern Vietnam, Veterinary Parasitology, 166, 314–320.

    Article  PubMed  CAS  Google Scholar 

  • Konrad, J.L, Moore, D.P, Crudeli, G., Caspe, S.G. Cano D.B., Leunda, M.R. Lischinsky, L. Regidor-Cerrillo, J. Odeón, A.C. Ortega-Mora, L.M. Echaide, I., Campero, C.M., 2012. Experimental inoculation of Neospora caninum in pregnant water buffalo, Veterinary Parasitology, 87, 72–78.

  • Levett, P.N., 2008. Minutes International Committee on Systematics of Prokaryotes. Sucommittee on the taxonomy of Leptospiraceae, International Journal of Systematic and Evolutionary Microbiology, 58, 1049–1050.

    Article  Google Scholar 

  • Moré, G., Basso, W., Bacigalupe, D., Venturini, M.C., Venturini, L., 2008. Diagnosis of Sarcocystis cruzi, Neospora caninum, and Toxoplasma gondii infections in cattle, Parasitology Research, 102, 671–675.

    Article  PubMed  Google Scholar 

  • Moré, G., Abrahamovich, P., Jurado, S., Bacigalupe, D., Marin, J.C., Rambeaud, M., Venturini, L., Venturini, M.C., 2011. Prevalence of Sarcocystis spp. in Argentinean cattle, Veterinary Parasitology, 177, 162–165.

    Article  PubMed  Google Scholar 

  • Nicoletti, P., 1992. An evaluation of serologic tests used to diagnose brucellosis in buffaloes (Bubalus bubalis), Tropical Animal Health and Production, 24, 40–44.

    Article  PubMed  CAS  Google Scholar 

  • Persad, A., Charles, R., Adesiyun, A.A., 2011. Frequency of Toxoplasmosis in Water Buffalo (Bubalus bubalis) in Trinidad. Veterinary Medicine International, doi: 10.4061/2011/705358.

    PubMed  Google Scholar 

  • OIE Terrestrial Manual, 2008. http://www.oie.int/fileadmin/Home/eng/Health_standards/tahm/2.01.09_LEPTO.pdf

  • OIE Terrestrial Manual, 2009. http://www.oie.int/fileadmin/Home/eng/Health_standards/tahm/2.04.03_BOVINE_BRUCELL.pdf

  • Oryan, A,, Ahmadi, N., Mousavi, S.M., 2010. Prevalence, biology, and distribution pattern of Sarcocystis infection in water buffalo (Bubalus bubalis) in Iran. Tropical Animal Health and Production, 42, 1513–1518.

    Article  PubMed  Google Scholar 

  • Suwancharoen, D., Chaisakdanugull, Y., Thanapongtharm, W., Yoshida, S., 2013. Serological survey of leptospirosis in livestock in Thailand. Epidemiology and Infection, 11, 1–9.

    Google Scholar 

  • Xiang, Z., He, Y., Zhao, H., Rosenthal, B.M., Dunams, D.B., Li, X., Zuo, Y., Feng, G., Cui, L., Yang, Z., 2011. Sarcocystis cruzi: Comparative studies confirm natural infections of buffaloes, Experimental Parasitology, 127, 460–466.

    Article  PubMed  Google Scholar 

  • Yu, J., Xia, Z., Liu, Q., Liu, J., Ding, J., Zhang, W., 2007. Seroepidemiology of Neospora caninum and Toxoplasma gondii in cattle and water buffaloes (Bubalus bubalis) in the People’s Republic of China, Veterinary Parasitology, 143, 79–85.

    Article  PubMed  Google Scholar 

Download references

Acknowledgments

We thank Drs. Bacigalupe, Moré, and Pardini as well as technicians at INTA Balcarce for their assistance and laboratory support. This work was supported by special grants from PICT 2412 FONCYT and national project AESA 203971 from INTA, Argentina.

Conflict of Interest

None of the authors of this paper has a financial or personal relationship with other people or organizations that could inappropriately influence or bias the content of this article.

Author information

Authors and Affiliations

  1. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina

    José L. Konrad, Lucía M. Campero & Dadin P. Moore

  2. Facultad de Ciencias Veterinarias, UNNE, Corrientes, Argentina

    José L. Konrad & Gustavo A. Crudeli

  3. Laboratorio de Inmunoparasitología, Facultad de Ciencias Veterinarias, UNLP, La Plata, Argentina

    Lucía M. Campero & María C. Venturini

  4. Instituto Nacional de Tecnología Agropecuaria (INTA), Buenos Aires, Argentina

    Gastón S. Caspe, Bibiana Brihuega, Graciela Draghi & Carlos M. Campero

Authors
  1. José L. Konrad
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Lucía M. Campero
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Gastón S. Caspe
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Bibiana Brihuega
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Graciela Draghi
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Dadin P. Moore
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Gustavo A. Crudeli
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. María C. Venturini
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Carlos M. Campero
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Dadin P. Moore.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Konrad, J.L., Campero, L.M., Caspe, G.S. et al. Detection of antibodies against Brucella abortus, Leptospira spp., and Apicomplexa protozoa in water buffaloes in the Northeast of Argentina. Trop Anim Health Prod 45, 1751–1756 (2013). https://doi.org/10.1007/s11250-013-0427-y

Download citation

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11250-013-0427-y

Keywords

Search

Navigation