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Use of reverse genetics to develop a novel marker porcine reproductive and respiratory syndrome virus

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Abstract

Nucleocapsid (N) protein of porcine reproductive and respiratory syndrome virus (PRRSV) is the most abundant viral structural protein with high immunogenicity. Previously, the nonessential sequences for virus infectivity were identified at both N and C terminal ends of N protein. Here, by means of reverse genetics, a marker virus (v7APMa) was generated with a mutant N protein that differs from the wild-type strains (vAPRRS, type 2 PRRSV). v7APMa shows stable inheritance in cell culture and the virologic characteristics of the marker virus in vitro showed that v7APMa replicates well as its parental strain. In the pig model, the v7APMa marker virus induced a similar level of anti-N protein antibodies and robust antibodies against the marker peptide, from 14 days post infection. In addition, a peptide-based ELISA was developed to detect the specific antibodies for the introduced 7APMa peptide. This approach, using a rationally designed marker virus, provides a new potential mutant basis for further development of PRRSV novel vaccines.

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Acknowledgments

This study was partially supported from the Natural Sciences Foundation of China (#30972204, #30901078) and the EU Frame 7 Program Project (245141) to S. Y.

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Authors and Affiliations

  1. College of Veterinary Medicine, Nanjing Agricultural University, No. 1, Weigang Street, Xuanwu District, Nanjing, 210095, People’s Republic of China

    Tao Lin, Xiangrui Li, Huochun Yao, Lichang Sun & Wenliang Li

  2. Department of Swine Infectious Diseases, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 518, Ziyue Road, Minhang District, Shanghai, 200241, People’s Republic of China

    Tao Lin, Zuzhang Wei, Feifei Tan, Runxia Liu, Lichang Sun, Rong Zhang, Jiaqi Lu, Guangzhi Tong & Shishan Yuan

Authors
  1. Tao Lin
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  2. Xiangrui Li
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  4. Zuzhang Wei
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Corresponding authors

Correspondence to Xiangrui Li or Guangzhi Tong.

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Lin, T., Li, X., Yao, H. et al. Use of reverse genetics to develop a novel marker porcine reproductive and respiratory syndrome virus. Virus Genes 45, 548–555 (2012). https://doi.org/10.1007/s11262-012-0812-z

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  • DOI: https://doi.org/10.1007/s11262-012-0812-z

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