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The complete genome of a putative endornavirus identified in yerba mate (Ilex paraguariensis St. Hil.)

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Abstract

We present the first report of a virus infecting the subtropical tree crop yerba mate (Ilex paraguariensis St. Hil.). Total RNA purification, followed by next-generation sequencing, transcripts assembly and annotation, resulted in the identification of a new endornavirus species infecting yerba mate. The complete sequence of the linear dsRNA viral genome is 13,954-nt long, contains a single 13,743 nt ORF, and presents a 149 nt 5′UTR and a 61 nt 3′UTR. The predicted ORF encodes a 4,581 aa polypeptide with a UDP-glucose glycosyl-transferase, a capsular polysaccharide synthesis protein, and a RNA-dependent RNA polymerase domain. The name yerba mate endornavirus is proposed for the identified virus. Due to the intriguing peculiarities of this virus family, and the complete lack of the yerba mate virus literature, we consider that the information reported here will be helpful in leading to a new and needed attention to this important topic and crop.

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Authors and Affiliations

  1. Instituto de Patología Vegetal, Centro de Investigaciones Agropecuarias, Instituto Nacional de Tecnología Agropecuaria (IPAVE-CIAP-INTA), X5020ICA, Córdoba, Argentina

    Humberto J. Debat & Daniel A. Ducasse

  2. Instituto de Biología Subtropical, Universidad Nacional de Misiones (IBS-UNaM-CONICET), 3300, Posadas, Misiones, Argentina

    Mauro Grabiele, Patricia M. Aguilera & Dardo A. Marti

  3. Estación Experimental Agropecuaria Cerro Azul, Instituto Nacional de Tecnología Agropecuaria (EEA Cerro Azul-INTA), 3313, Cerro Azul, Misiones, Argentina

    Rosana Bubillo

  4. Instituto de Biotecnología Misiones, Facultad de Ciencias Exactas Químicas y Naturales, Universidad Nacional de Misiones (INBIOMIS-FCEQyN-UNaM), 3300, Posadas, Misiones, Argentina

    Pedro D. Zapata

Authors
  1. Humberto J. Debat
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  2. Mauro Grabiele
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  3. Patricia M. Aguilera
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  4. Rosana Bubillo
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  5. Pedro D. Zapata
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  6. Dardo A. Marti
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  7. Daniel A. Ducasse
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Corresponding author

Correspondence to Humberto J. Debat.

Additional information

Nucleotide sequence accession number: The genome sequence of the proposed yerba mate endornavirus has been deposited in NCBI GenBank under accession no. KJ634409.

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Debat, H.J., Grabiele, M., Aguilera, P.M. et al. The complete genome of a putative endornavirus identified in yerba mate (Ilex paraguariensis St. Hil.). Virus Genes 49, 348–350 (2014). https://doi.org/10.1007/s11262-014-1096-2

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  • DOI: https://doi.org/10.1007/s11262-014-1096-2

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