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Biological characteristics and genomic analysis of a Stenotrophomonas maltophilia phage vB_SmaS_BUCT548

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Abstract

Stenotrophomonas maltophilia (hereinafter referred to as S. maltophilia) has developed into an important opportunistic pathogenic bacterium, which is prevalent in nosocomial and community infections, and has adverse effects on patients with a compromised immune system. Phage vB_SmaS_BUCT548 was isolated from sewage of Beijing 307 Hospital with S. maltophilia (strain No.824) as a host. Phage morphology was observed by transmission electron microscopy and its biological and genomic characteristics were determined. The electron microscope shows that the bacteriophage belonged to the Siphoviridae and MOI is 0.001. One-step growth curve shows that the incubation period is 30 min and the burst size is 134 PFU/Cell. The host range is relatively wide and it can lysis 11of 13 S. maltophilia strains. Next-Generation Sequencing (NGS) results show that the genome sequence is a dsDNA with 62354 bp length, and the GC content is 56.3% (GenBank: MN937349). One hundred and two online reading frames (ORFs) are obtained after RAST online annotation and the BlastN nucleic acid comparison shows that the phage had low homology with other phages in NCBI database. This study reports a novel S. maltophilia phage named vB_SmaS_BUCT548, which has a short incubation period, strong lytic ability, and a wide host range. The main characteristic of this bacteriophage is the novelty of the genomic sequence and the analysis of the other characteristics provides basic data for further exploring the interaction mechanism between the phage and the host.

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Data availability

The genome sequence of the phage can be obtained from GenBank (GenBank: MN937349). All data and materials in the study are available.

Code availability

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Funding

This research was supported by grants from the National Key Research and Development Program (2018YFA0903000), Key Project of Beijing University of Chemical Technology (No. XK1803-06), Fundamental Research Funds for Central Universities (No. BUCTRC201917, No. BUCTZY2022).

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Author notes

  1. Wenjing Zhang, Rongrong Zhang and Yunjia Hu have contributed equally to this work.

Authors and Affiliations

  1. School of Public Health, Lanzhou University, Lanzhou, 730000, China

    Wenjing Zhang, Rongrong Zhang & Hui Liu

  2. Beijing Advanced Innovation Center for Soft Matter Science and Engineering (BAIC-SM), College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, 100029, China

    Wenjing Zhang, Rongrong Zhang, Yunjia Hu, Yujie Liu, Liqin Wang, Xiaoping An, Lihua Song, Huahao Fan & Yigang Tong

  3. Medical College Qingdao University, Qingdao, 266071, China

    Liqin Wang

  4. Academy of Military Medical Sciences, Beijing, 100085, China

    Taoxing Shi

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  1. Wenjing Zhang
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Contributions

HL, YT, and HF conceived and designed experiments and critically evaluated the manuscript. WZ and HF drafted the manuscript, RZ extracted the phage nucleotide and conducted the sequencing experiments. WZ and YH isolated and identified the phage and conducted the biological characterization experiments. YL, LW, XA, LS, and TS were involved the phage bio-informatics analysis. All authors read and approved the final manuscript.

Corresponding authors

Correspondence to Taoxing Shi, Huahao Fan, Yigang Tong or Hui Liu.

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Zhang, W., Zhang, R., Hu, Y. et al. Biological characteristics and genomic analysis of a Stenotrophomonas maltophilia phage vB_SmaS_BUCT548. Virus Genes 57, 205–216 (2021). https://doi.org/10.1007/s11262-020-01818-5

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