Abstract
Indian cassava mosaic virus (ICMV), responsible for the cassava mosaic disease in India, harbours two circular genomic components, DNA-A and DNA-B; the former being responsible for the encapsidation and replication and the latter for intra- and inter-cellular movement of the viral DNA. Two proteins, encoded by DNA-B, the movement protein (MP) and the nuclear shuttle protein (NSP), act in concert on the newly replicated viral DNA to move it from the nucleus to the cell periphery. To map the functional domains of NSP, the intra-cellular localization of its full-length protein and deletion derivatives was studied in the epidermal cells of detached leaves of the laboratory host plant, Nicotiana benthamiana, where the target proteins were transiently expressed as GFP fusions. This analysis revealed domains for nuclear localization at the N-terminus, as well as for localization towards the cell periphery both at the C-terminus and center of the NSP.
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Acknowledgements
The facilities provided at University of Stuttgart by Prof. Holger Jeske for part of this work are gratefully acknowledged. The study was funded by EU INCO-DEV Grant ICA-CT-2000-30001. BLP acknowledges Research Fellowship from Council for Scientific and Industrial Research, New Delhi, the DAAD Scholarship and the financial support of Vater-ünd-Sohn-Eiselen-Stiftung. Help in photography from Frau Sigrid Kober is gratefully acknowledged.
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ID and BLP conceived and designed the study, BLP performed the experiments and drafted the manuscript, ID supervised the study and corrected the manuscript.
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Patil, B.L., Dasgupta, I. Characterization of the functional domains of nuclear shuttle protein (NSP) of Indian cassava mosaic virus using green fluorescent protein as reporter. Virus Genes 58, 308–318 (2022). https://doi.org/10.1007/s11262-022-01909-5
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DOI: https://doi.org/10.1007/s11262-022-01909-5