Abstract
Both domestic and industrial effluent treatments contain or produce nitrogen loading during the treatment process. It is important to seek the removal of nitrogen while maintaining the design of existing systems, which are usually composed by the association of anaerobic and aerobic reactors. Thus, in this research, an anaerobic filter (AF) and an upflow anaerobic sludge blanket (UASB) reactors were fed with synthetic effluent enriched with nitrate to compare how these reactors would behave if they became denitrifying reactors. With the application of 100.0 mg NO3−-NL−1, the AF presented better efficiency. With respect to the biogas production, the composition was significantly altered: from CH4 and CO2 concentrations close to 70% and 13% without NO3N addition to N2 concentration higher than 85% with addition of 100.0 mg NO3−-NL−1. The UASB hydrodynamic profile was modified due to an increase in the mixing behavior along the denitrification stages by biogas production. This was not observed in the AF due to the presence of the support media, which was also responsible for ensuring a greater capacity to withstand denitrification without organic matter being carried out of the system.
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Aamir, S., Sutar, S., Singh, S. K., & Baghela, A. (2015). A rapid and efficient method of fungal genomic DNA extraction, suitable for PCR based molecular methods. Plant Pathology & Quarantine, 5(2), 74–81. https://doi.org/10.5943/ppq/5/2/6.
Al-Zreiqat, I., Abbassi, B., Headley, T., Nivala, J., van Afferden, M., & Müller, R. A. (2018). Influence of septic tank attached growth media on total nitrogen removal in a recirculating vertical flow constructed wetland for treatment of domestic wastewater. Ecological Engineering, 118, 171–178. https://doi.org/10.1016/J.ECOLENG.2018.05.013.
An, Y., Yang, F., Chua, H. C., Wong, F. S., & Wu, B. (2008). The integration of methanogenesis with shortcut nitrification and denitrification in a combined UASB with MBR. Bioresource Technology, 99(9), 3714–3720. https://doi.org/10.1016/J.BIORTECH.2007.07.020.
Andalib, M., Nakhla, G., McIntee, E., & Zhu, J. (2011). Simultaneous denitrification and methanogenesis (SDM): review of two decades of research. Desalination, 279(1–3), 1–14. https://doi.org/10.1016/J.DESAL.2011.06.018.
APHA, AWWA, & WEF. (2012). Standard methods for examination of water and wastewater (22nd ed.). Washington: American Public Health Association.
Azevedo, L. S., Castro, I. M. P., Leal, C. D., Araújo, J. C., & Chernicharo, C. A. L. (2018). Performance and bacterial diversity of bioreactors used for simultaneous removal of sulfide, solids and organic matter from UASB reactor effluents. Water Science and Technology, 78(6), 1312–1323. https://doi.org/10.2166/wst.2018.403.
Browner, C. M., Fox, A. J. C., Grubbs, G. H., Rubin, M., Barash, S. Z., Ebner, M. C., & Tudor, L. (2000). Development document for the proposed effluent limitations guidelines and standards for the metal products & Machinery Point Source Category.
Chang, D., Seo, S. C., & Hong, K. H. (2004). Pre Denitri. and post nitri in Adv Ww treat.Pdf. Journal of Industrial and Engineering Chemistry, 10(3), 354–360.
Chernicharo, C. A. L. (2007). Anaerobic reactors. IWA Publishing.
Chernicharo, C. A. L., van Lier, J. B., Noyola, A., & Bressani Ribeiro, T. (2015). Anaerobic sewage treatment: state of the art, constraints and challenges. Reviews in Environmental Science and Bio/Technology, 14(4), 649–679. https://doi.org/10.1007/s11157-015-9377-3.
Council Directive 91/271/EEC. (1991). Council directive 91/271/EEC of 21 May 1991 concerning urban waste water treatment, The Council of the European Communities.
Cruz, L., Stefanutti, R., Coraucci Filho, B., & Tonetti, A. (2013). Coconut shells as filling material for anaerobic filters. SpringerPlus, 2(1), 655. https://doi.org/10.1186/2193-1801-2-655.
Eiroa, M., Kennes, C., & Veiga, M. C. (2004). Formaldehyde and urea removal in a denitrifying granular sludge blanket reactor. Water Research, 38(16), 3495–3502. https://doi.org/10.1016/J.WATRES.2004.04.055.
Ersahin, M. E., Ozgun, H., Dereli, R. K., & Ozturk, I. (2011). Anaerobic treatment of industrial effluents: an overview of applications. In Waste water-treatment and reutilization. InTech.
Fang, H. H. P. (2010). Environmental anaerobic technology: applications and new developments. Imperial College Press.
Gavrilescu, M., & Macoveanu, M. (2000). Attached-growth process engineering in wastewater treatment. Bioprocess Engineering, 23(1), 95–106. https://doi.org/10.1007/s004490050030.
Han, Y., Liu, J., Guo, X., & Li, L. (2012). Micro-environment characteristics and microbial communities in activated sludge flocs of different particle size. Bioresource Technology, 124, 252–258. https://doi.org/10.1016/J.BIORTECH.2012.08.008.
Hanaki, K., & Polprasert, C. (1989). Contribution of methanogenesis to denitrification with an upflow filter. Research Journal of the Water Pollution Control Federation. https://doi.org/10.2307/25043777.
Hug, L. A., Castelle, C. J., Wrighton, K. C., Thomas, B. C., Sharon, I., Frischkorn, K. R., et al. (2013). Community genomic analyses constrain the distribution of metabolic traits across the Chloroflexi phylum and indicate roles in sediment carbon cycling. Microbiome, 1(1), 22. https://doi.org/10.1186/2049-2618-1-22.
Jin, B., & Lant, P. (2004). Flow regime, hydrodynamics, floc size distribution and sludge properties in activated sludge bubble column, air-lift and aerated stirred reactors. Chemical Engineering Science, 59(12), 2379–2388. https://doi.org/10.1016/J.CES.2004.01.061.
Jin, X., Wang, F., Liu, G., & Yan, N. (2012). A key cultivation technology for denitrifying granular sludge. Process Biochemistry, 47(7), 1122–1128. https://doi.org/10.1016/J.PROCBIO.2012.04.001.
Kampschreur, M. J., Temmink, H., Kleerebezem, R., Jetten, M. S. M., & van Loosdrecht, M. C. M. (2009). Nitrous oxide emission during wastewater treatment. Water Research, 43(17), 4093–4103. https://doi.org/10.1016/J.WATRES.2009.03.001.
Khan, S. J., Ilyas, S., Javid, S., Visvanathan, C., & Jegatheesan, V. (2011). Performance of suspended and attached growth MBR systems in treating high strength synthetic wastewater. Bioresource Technology, 102(9), 5331–5336. https://doi.org/10.1016/J.BIORTECH.2010.09.100.
Klas, S., Mozes, N., & Lahav, O. (2006). A conceptual, stoichiometry-based model for single-sludge denitrification in recirculating aquaculture systems. Aquaculture, 259(1–4), 328–341. https://doi.org/10.1016/J.AQUACULTURE.2006.05.048.
Kodera, T., Akizuki, S., & Toda, T. (2017). Formation of simultaneous denitrification and methanogenesis granules in biological wastewater treatment. Process Biochemistry, 58, 252–257. https://doi.org/10.1016/J.PROCBIO.2017.04.038.
Kreft, P., Scheible, O. K., & Venosa, A. (1986). Hydraulic studies and cleaning evaluations of ultraviolet disinfection units. Journal (Water Pollution Control Federation). https://doi.org/10.2307/25043146.
Leal, C. D., Pereira, A. D., Nunes, F. T., Ferreira, L. O., Coelho, A. C. C., Bicalho, S. K., et al. (2016). Anammox for nitrogen removal from anaerobically pre-treated municipal wastewater: Effect of COD/N ratios on process performance and bacterial community structure. Bioresource Technology, 211, 257–266. https://doi.org/10.1016/J.BIORTECH.2016.03.107.
Levenspiel, O. (1999). Chemical reaction engineering. Industrial & Engineering Chemistry Research, 38(11), 4140–4143.
Leverenz, H. L., Haunschild, K., Hopes, G., Tchobanoglous, G., & Darby, J. L. (2010). Anoxic treatment wetlands for denitrification. Ecological Engineering, 36(11), 1544–1551. https://doi.org/10.1016/J.ECOLENG.2010.03.014.
Lim, S. J., & Fox, P. (2011). A kinetic analysis and experimental validation of an integrated system of anaerobic filter and biological aerated filter. Bioresource Technology, 102(22), 10371–10376. https://doi.org/10.1016/J.BIORTECH.2011.09.005.
Lu, H., Chandran, K., & Stensel, D. (2014). Microbial ecology of denitrification in biological wastewater treatment. Water Research, 64, 237–254. https://doi.org/10.1016/J.WATRES.2014.06.042.
Mac Conell, E. F. A., Almeida, P. G. S., Martins, K. E. L., Araújo, J. C., & Chernicharo, C. A. L. (2015). Bacterial community involved in the nitrogen cycle in a down-flow sponge-based trickling filter treating UASB effluent. Water Science & Technology, 72(1), 116. https://doi.org/10.2166/wst.2015.154.
Mao, C., Feng, Y., Wang, X., & Ren, G. (2015). Review on research achievements of biogas from anaerobic digestion. Renewable and Sustainable Energy Reviews, 45, 540–555. https://doi.org/10.1016/J.RSER.2015.02.032.
Mateo-Sagasta Dávila, J., Khassab, G., Klapwijk, A., & van Lier, J. B. (2009). Combination of methanogenesis and denitrification in a UASB reactor for water reclamation applied to small agglomerations. Desalination and Water Treatment, 4(1–3), 177–182. https://doi.org/10.5004/dwt.2009.373.
Méndez-Romero, D. C., López-López, A., Vallejo-Rodríguez, R., & León-Becerril, E. (2011). Hydrodynamic and kinetic assessment of an anaerobic fixed-bed reactor for slaughterhouse wastewater treatment. Chemical Engineering and Processing: Process Intensification, 50(3), 273–280. https://doi.org/10.1016/J.CEP.2011.02.002.
Muyzer, G., de Waal, E. C., & Uitterlinden, A. G. (1993). Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA. Applied and Environmental Microbiology, 59(3), 695–700.
Muyzer, G. T., Brinkhoff, U., Nübel, C., Santegoeds, H., & Schäfer, C. (1997). Denaturing gradient gel electrophoresis (DGGE) in microbial ecology. Molecular Microbial Ecology Manual, Kluwer Academics Publishers. Dordrecht, The Netherlands, 1–27.
Niu, W., Guo, J., Lian, J., Ngo, H. H., Li, H., Song, Y., et al. (2018). Effect of fluctuating hydraulic retention time (HRT) on denitrification in the UASB reactors. Biochemical Engineering Journal, 132, 29–37. https://doi.org/10.1016/J.BEJ.2017.12.017.
Noyola, A., Padilla-Rivera, A., Morgan-Sagastume, J. M., Güereca, L. P., & Hernández-Padilla, F. (2012). Typology of municipal wastewater treatment technologies in Latin America. CLEAN - Soil, Air, Water, 40(9), 926–932. https://doi.org/10.1002/clen.201100707.
Pagáčová, P., Galbová, K., Drtil, M., & Jonatová, I. (2010). Denitrification in USB reactor with granulated biomass. Bioresource Technology, 101(1), 150–156. https://doi.org/10.1016/J.BIORTECH.2009.08.021.
Parawira, W., Murto, M., Zvauya, R., & Mattiasson, B. (2006). Comparative performance of a UASB reactor and an anaerobic packed-bed reactor when treating potato waste leachate. Renewable Energy, 31(6), 893–903. https://doi.org/10.1016/J.RENENE.2005.05.013.
Peña, M. R., Mara, D. D., & Avella, G. P. (2006). Dispersion and treatment performance analysis of an UASB reactor under different hydraulic loading rates. Water Research, 40(3), 445–452. https://doi.org/10.1016/J.WATRES.2005.11.021.
Perry, J. H. (1950). Chemical engineers’ handbook. ACS Publications.
Polprasert, C., & Park, H. S. (1986). Effluent denitrification with anaerobic filters. Water Research, 20(8), 1015–1021. https://doi.org/10.1016/0043-1354(86)90044-8.
Quaff, A. R., & Guha, S. (2011). Evaluation of mixing and performance of lab-scale upflow anaerobic sludge blanket reactors treating domestic wastewater. Journal of Environmental Engineering, 137(5), 322–331. https://doi.org/10.1061/(ASCE)EE.1943-7870.0000333.
Renuka, R., Mariraj Mohan, S., & Amal Raj, S. (2016). Hydrodynamic behaviour and its effects on the treatment performance of panelled anaerobic baffle-cum filter reactor. International journal of Environmental Science and Technology, 13(1), 307–318. https://doi.org/10.1007/s13762-015-0824-z.
Robarge, W. P., Edwards, A., & Johnson, B. (1983). Water and waste water analysis for nitrate via nitration of salicylic acid. Communications in Soil Science and Plant Analysis, 14(12), 1207–1215. https://doi.org/10.1080/00103628309367444.
Rosa, A. P., Conesa, J. A., Fullana, A., Melo, G. C. B., Borges, J. M., & Chernicharo, C. A. L. (2016). Energy potential and alternative usages of biogas and sludge from UASB reactors: case study of the Laboreaux wastewater treatment plant. Water Science and Technology, 73(7), 1680–1690. https://doi.org/10.2166/wst.2015.643.
Rosa, A. P., Chernicharo, C. A. L., Lobato, L. C. S., Silva, R. V., Padilha, R. F., & Borges, J. M. (2018). Assessing the potential of renewable energy sources (biogas and sludge) in a full-scale UASB-based treatment plant. Renewable Energy, 124, 21–26. https://doi.org/10.1016/J.RENENE.2017.09.025.
Saliba, P. D., & Von Sperling, M. (2017). Performance evaluation of a large sewage treatment plant in Brazil, consisting of an upflow anaerobic sludge blanket reactor followed by activated sludge. Water Science and Technology, 76(8), 2003–2014. https://doi.org/10.2166/wst.2017.284.
Saliling, W. J. B., Westerman, P. W., & Losordo, T. M. (2007). Wood chips and wheat straw as alternative biofilter media for denitrification reactors treating aquaculture and other wastewaters with high nitrate concentrations. Aquacultural Engineering, 37(3), 222–233. https://doi.org/10.1016/J.AQUAENG.2007.06.003.
Sánchez, E., Milán, Z., Borja, R., Weiland, P., & Rodriguez, X. (1995). Piggery waste treatment by anaerobic digestion and nutrient removal by ionic exchange. Resources, Conservation and Recycling, 15(3–4), 235–244. https://doi.org/10.1016/0921-3449(95)00033-X.
Shen, Z., Zhou, Y., Hu, J., & Wang, J. (2013). Denitrification performance and microbial diversity in a packed-bed bioreactor using biodegradable polymer as carbon source and biofilm support. Journal of Hazardous Materials, 250–251, 431–438. https://doi.org/10.1016/J.JHAZMAT.2013.02.026.
Show, K.-Y., & Tay, J.-H. (1999). Influence of support media on biomass growth and retention in anaerobic filters. Water Research, 33(6), 1471–1481. https://doi.org/10.1016/S0043-1354(98)00352-2.
Silva, J. C. P., Tonetti, A. L., Leonel, L. P., & Costa, A. (2015). Denitrification on upflow-anaerobic filter filled with coconut shells (Cocos nucifera). Ecological Engineering, 82, 474–479. https://doi.org/10.1016/J.ECOLENG.2015.05.007. https://www.sciencedirect.com/science/article/pii/S0925857415300392?via%3Dihub
Singh, N. K., Kazmi, A. A., & Starkl, M. (2015). A review on full-scale decentralized wastewater treatment systems: techno-economical approach. Water Science and Technology, 71(4), 468–478. https://doi.org/10.2166/wst.2014.413.
Souza, C. L., Chernicharo, C. A. L., & Aquino, S. F. (2011). Quantification of dissolved methane in UASB reactors treating domestic wastewater under different operating conditions. Water Science and Technology, 64(11), 2259–2264. https://doi.org/10.2166/wst.2011.695.
Stazi, V., & Tomei, M. C. (2018). Enhancing anaerobic treatment of domestic wastewater: state of the art, innovative technologies and future perspectives. Science of the Total Environment, 635, 78–91. https://doi.org/10.1016/J.SCITOTENV.2018.04.071.
Tchobanoglous, G., & Schroeder, E. E. (1985). Water quality: characteristics, modeling, modification. Reading: Addison-Wesley Pub. Co. https://www.osti.gov/biblio/5887635. Accessed 11 March 2019.
Tchobanoglous, G., Burton, F. L., Stensel, H. D., et. al. (2003). Metcalf & Eddy wastewater engineering: treatment and reuse. International Edition. McGrawHill, 4, 361–411.
Tonetti, A. L., Coraucci Filho, B., Bertoncini, E. I., Oliveira, R. A., & Stefanutti, R. (2010). Avaliação de um sistema simplificado de tratamento de esgotos visando a utilização em áreas rurais. Revista Brasileira de Engenharia Agrícola e Ambiental, 14(2), 227–234. https://doi.org/10.1590/S1415-43662010000200015.
Tonetti, A. L., Coraucci Filho, B., Guimarães, J. R., Fadini, P. S., & Nicolau, C. E. (2013). Desnitrificação em um sistema simplificado de tratamento de esgoto. Engenharia Sanitaria e Ambiental, 18(4), 381–392. https://doi.org/10.1590/S1413-41522013000400010.
USEPA. (2009). Nutrient control design manual: State of technology review report.
Haandel, A. C. van, & Lettinga, G. (1994). Anaerobic sewage treatment: a practical guide for regions with a hot climate. Anaerobic sewage treatment: a practical guide for regions with a hot climate.
Von Sperling, M., & Chernicharo, C. A. L. (2005). Biological wastewater treatment in warm climate regions. IWA.
Wunderlin, P., Mohn, J., Joss, A., Emmenegger, L., & Siegrist, H. (2012). Mechanisms of N2O production in biological wastewater treatment under nitrifying and denitrifying conditions. Water Research, 46(4), 1027–1037. https://doi.org/10.1016/J.WATRES.2011.11.080.
Xue, Y., Guo, J., Lian, J., Zhang, Y., Zhang, C., & Zhao, Y. (2016). Effects of a higher hydraulic shear force on denitrification granulation in upflow anoxic sludge blanket reactors. Biochemical Engineering Journal, 105, 136–143. https://doi.org/10.1016/J.BEJ.2015.09.010.
Zhao, L., Guo, J., Lian, J., Guo, Y., Yue, L., Gou, C., et al. (2015). Study of the dynamics and material transformation characteristics of nitrite denitrification in UASB. Biotechnology & Biotechnological Equipment, 29(5), 907–914. https://doi.org/10.1080/13102818.2015.1050789.
Zheng, M. X., Wang, K. J., Zuo, J. E., Yan, Z., Fang, H., & Yu, J. W. (2012). Flow pattern analysis of a full-scale expanded granular sludge bed-type reactor under different organic loading rates. Bioresource Technology, 107, 33–40. https://doi.org/10.1016/J.BIORTECH.2011.11.102.
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The authors would like to thank CNPq (Brazilian National Council for Scientific and Technological Development, process number 311275/2015-0) and FAPESP (São Paulo Research Foundation, process number 2017/07490-4) for financing this study. The authors would also like to acknowledge the service of the Writing Space/General Coordination of UNICAMP for helping translate the original manuscript.
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Magalhães, T.M., Duarte, N.C., de Alencar Neves, T. et al. The Challenge of Making Wastewater Treatment Plants Composed by Anaerobic Reactors Capable of Removing Nitrogen. Water Air Soil Pollut 230, 234 (2019). https://doi.org/10.1007/s11270-019-4300-0
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DOI: https://doi.org/10.1007/s11270-019-4300-0