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Genetic mapping of the dominant gene controlling weeping habit in Japanese chestnut (Castanea crenata Sieb. et Zucc.)

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Abstract

Trees with weeping traits have been prized for centuries because of their unique shape. Weeping trees of various species have been selected and propagated for their ornamental appeal as landscape plants, but there is limited information on the genes that control weeping. The genetic control of weeping in Japanese chestnut (Castanea crenata Sieb. et Zucc.) is reported to involve a dominant or recessive gene depending on the genetic background. In every case examined to date, weeping is controlled by a single gene of unknown location. Here, we identified the exact position of the dominant gene controlling the weeping trait in the Japanese chestnut. Using the genome scanning approach, which can cost-effectively screen for markers linked to traits, we first mapped this gene, referred to here as Weep, to the middle of linkage group L. Then, we developed new simple sequence repeat (SSR) markers using the genomic resources to enrich the target region. Using these markers and previously reported markers, we constructed the linkage group covering most of the chromosome (60.9 cM) with an average marker density of 4.7 cM per marker. Weep was located within a 3.0-cM genome region. SSR marker CmSca01437 co-segregated with Weep in all 138 F1 plantlets of a Tsukuba-38 × 668-6 cross. Our identification of the exact locus of the dominant weeping gene in Japanese chestnut, in conjunction with previous studies of recessive weeping genes in this and other tree species, furthers our understanding of the genetic mechanism of the weeping trait.

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Data archiving statement

This paper uses existing public data, and there is no new data to register. Primers have been newly designed, and all of them are shown in the table in the manuscript.

Data and materials availability

The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.

Code availability

The name, version, and parameters of the software used in this study are described in the Materials and Methods section.

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Acknowledgements

We are grateful to Mss. N. Yagihashi, H. Takahashi, and N. Minagawa of our laboratory for their technical assistance. Some computations were partially performed on the NIG supercomputer at ROIS (Research Organization of Information and Systems), National Institute of Genetics, Japan.

Funding

This work was funded exclusively by the Institute of Fruit Tree and Tea Science, NARO.

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Authors and Affiliations

  1. Institute of Fruit Tree and Tea Science, National Agriculture and Food Research Organization (NARO), 2-1 Fujimoto, Tsukuba, Ibaraki, 305-8605, Japan

    Shingo Terakami, Sogo Nishio, Hidenori Kato, Norio Takada, Toshihiro Saito & Toshiya Yamamoto

Authors
  1. Shingo Terakami
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  2. Sogo Nishio
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  3. Hidenori Kato
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  4. Norio Takada
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  5. Toshihiro Saito
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  6. Toshiya Yamamoto
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Correspondence to Shingo Terakami.

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Supplementary Information

Supplemental Fig. 1

Overview of GSA. (a) Segregation of the weeping parent’s alleles of the marker conforms to 1:1 if the selected SSR marker is not linked to the Weep gene. An example of the segregation of the two alleles (pink and blue) of a hypothetical marker, Marker A, which is not linked to Weep, is shown. (b) Deviation from the 1:1 segregation is found if the selected SSR marker is linked to the Weep gene. An example of the segregation of the two alleles (green and yellow) of a hypothetical marker, Marker B, which is linked to the Weep gene, is shown (PDF 23 kb)

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Terakami, S., Nishio, S., Kato, H. et al. Genetic mapping of the dominant gene controlling weeping habit in Japanese chestnut (Castanea crenata Sieb. et Zucc.). Tree Genetics & Genomes 17, 16 (2021). https://doi.org/10.1007/s11295-021-01501-2

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