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Assessing the ratio of Bacillus spores and vegetative cells by shotgun proteomics

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Abstract

Mass spectrometry for rapid identification of microorganisms is expanding over the last years because this approach is quick. This methodology provides a decisive interest to fight against bioterrorism as it is applicable whatever the pathogen to be considered and often allows subtyping which may be crucial for confirming a massive and widespread attack with biological agents. Here, we present a methodology based on next-generation proteomics and tandem mass spectrometry for discovering numerous protein biomarkers allowing the discrimination of spores and vegetative cells of Bacillus atrophaeus, a biowarfare simulant. We propose a global quantitative evaluation of the two groups of discriminant biomarkers based on their aggregated normalized spectral abundance factors.

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Acknowledgments

We thank Jean-Charles Gaillard and Guylaine Miotello for invaluable technical help with the mass spectrometry platform.

Funding

This work was funded in part by the French joint ministerial program of R&D against CBRNE threats and the ANR program “Phylopeptidomics” (ANR-17-CE18-0023-01). CM was supported by DGA and CEA

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Authors and Affiliations

  1. Laboratoire Innovations technologiques pour la Détection et le Diagnostic (Li2D), Service de Pharmacologie et Immunoanalyse (SPI), CEA, INRA, 30207, Bagnols sur Cèze, France

    Charlotte Mappa, Olivier Pible, Jean Armengaud & Béatrice Alpha-Bazin

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  1. Charlotte Mappa
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  2. Olivier Pible
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  3. Jean Armengaud
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  4. Béatrice Alpha-Bazin
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Correspondence to Jean Armengaud.

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Responsible editor: Robert Duran

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Mappa, C., Pible, O., Armengaud, J. et al. Assessing the ratio of Bacillus spores and vegetative cells by shotgun proteomics. Environ Sci Pollut Res 28, 25107–25115 (2021). https://doi.org/10.1007/s11356-018-3341-z

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  • DOI: https://doi.org/10.1007/s11356-018-3341-z

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