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Chemical constituents from Inonotus obliquus and their antitumor activities

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Abstract

Four new lanostane-type triterpenes (inonotusanes D–G, 1–4), including a 24,25,26,27-tetranorlanostane, together with 11 known compounds (5–15), including 7 lanostane derivatives, 2 steroids and 2 aromatic compounds, were isolated from the sclerotia of Inonotus obliquus. Their structures were elucidated by 1D and 2D NMR spectroscopy and HRMS. To our knowledge, 1 is the first 24,25,26,27-tetranorlanostane-type triterpenoid from fungus, and this is the first time that 31-member lanostane-type triterpenes, 5 and 6, have been isolated from the sclerotia of I. obliquus instead of from its submerged culture. 7 and 8 are also new isolates of this genus. Compounds 1, 8, 12 and 13 exhibited strong cytotoxicity against the 4T1 (mouse breast cancer) cell line, with IC50 9.40, 7.79, 9.06 and 9.31 μM, respectively. 8, 12 and 13 also exhibited strong cytotoxicity against the the MCF-7 (human breast cancer) cell line, with IC50 8.35–9.01 μM.

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Authors and Affiliations

  1. Department of Natural Products Chemistry, School of Traditional Chinese Materia Medica, Key Laboratory of Structure-Based Drug Design & Discovery, Ministry of Education, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenhe District, Shenyang, 110016, China

    Fenqin Zhao, Guiyang Xia, Lixia Chen & Feng Qiu

  2. Institute of Pharmacy, Henan University, Jinming Street, Kaifeng, 475004, China

    Fenqin Zhao, Junli Zhao, Zhanfang Xie & Guang Han

  3. School of Chinese Materia Medica and Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, 312 Anshanxi Road, Nankai District, Tianjin, 300193, China

    Guiyang Xia & Feng Qiu

Authors
  1. Fenqin Zhao
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  2. Guiyang Xia
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  3. Lixia Chen
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  4. Junli Zhao
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  5. Zhanfang Xie
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  6. Feng Qiu
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  7. Guang Han
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Correspondence to Feng Qiu or Guang Han.

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Zhao, F., Xia, G., Chen, L. et al. Chemical constituents from Inonotus obliquus and their antitumor activities. J Nat Med 70, 721–730 (2016). https://doi.org/10.1007/s11418-016-1002-4

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  • DOI: https://doi.org/10.1007/s11418-016-1002-4

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