Abstract
Leaves of Aquilaria plants contain a variety of phenolic compounds such as iriflophenone glycosides, mangiferin, and genkwanin. Previous studies showed that Aquilaria leaf extracts exhibit many pharmacological activities, including antidiabetic and laxative effects. However, a few studies have reported differences in the chemical content and compositions of Aquilaria species. Here, three Aquilaria species were identified using matK and trnL–trnF sequences and their leaves were analyzed by HPLC and LC/MS. Comparison of the chemical components and α-glucosidase inhibition activity of the three species showed that the level of iriflophenone glycosides in A. rugosa was higher than in A. sinensis and A. crassna. There was no difference in the α-glucosidase inhibition activity of leaf extracts of the three species, but the strength of the inhibition activity can possibly be explained by the total sum of active compounds in the leaf extracts.
Data availability
The datasets generated and analyzed during the current study are available from the corresponding author on reasonable request.
References
Zhou M, Wang H, Suolangjiba KJ, Yu B (2008) Antinociceptive and anti-inflammatory activities of Aquilaria sinensis (Lour.) Gilg. Leaves extract. J Ethnopharmacol 117:345–350. https://doi.org/10.1016/j.jep.2008.02.005
Adam AZ, Lee SY, Mohamed R (2017) Pharmacological properties of agarwood tea derived from Aquilaria (Thymelaeaceae) leaves: an emerging contemporary herbal drink. J Herb Med 10:37–44. https://doi.org/10.1016/j.hermed.2017.06.002
Kakino M, Izuta H, Ito T, Tsumura K, Araki Y, Shimazawa M, Oyama M, Iinuma M, Hara H (2010) Agarwood induced laxative effects via acetylcholine receptors on loperamide-induced constipation in mice. Biosci Biotechnol Biochem 74:1550–1555. https://doi.org/10.1271/bbb.100122
Tay PY, Tan CP, Abas F, Yim HS, Ho CW (2014) Assessment of extraction parameters on antioxidant capacity, polyphenol content, epigallocatechin gallate (EGCG), epicatechin gallate (ECG) and iriflophenone 3-C-β-glucoside of agarwood (Aquilaria crassna) young leaves. Molecules 19:12304–12319. https://doi.org/10.3390/molecules190812304
Eissa MA, Hashim YZ, El-Kersh DM, Abd-Azziz SSS, Salleh MH, Muhammad Lokman Md, Isa NM, Warif A (2020) Metabolite profiling of Aquilaria malaccensis leaf extract using liquid chromatography-Q-TOF-mass spectrometry and investigation of its potential antilipoxygenase activity in-vitro. Processes 8:202. https://doi.org/10.3390/pr8020202
Feng J, Yang XW, Wang RF (2011) Bio-assay guided isolation and identification of α-glucosidase inhibitors from the leaves of Aquilaria sinensis. Phytochemistry 72:242–247. https://doi.org/10.1016/j.phytochem.2010.11.025
Hara H, Ise Y, Morimoto N, Shimazawa M, Ichihashi K, Ohyama M, Iinuma M (2008) Laxative effect of agarwood leaves and its mechanism. Biosci Biotechnol Biochem 72:335–345. https://doi.org/10.1271/bbb.70361
Le-Cong K, Kessler PJA, Eurlings M (2005) A new species of Aquilaria (Thymelaeaceae) from Vietnam. Blumea 50:135–141. https://doi.org/10.3767/000651905X623319
Lee SY, Ng WL, Mahat MN, Nazre M, Mohamed R (2016) DNA barcoding of the endangered Aquilaria (Thymelaeaceae) and its application in species authentication of agarwood products traded in the market. PLoS One. https://doi.org/10.1371/journal.pone.0154631
Tanaka S, Ito M (2020) DNA barcoding for identification of agarwood source species using trnL-trnF and matK DNA sequences. J Nat Med 74:42–50. https://doi.org/10.1007/s11418-019-01338-z
Ito T, Kakino M, Tazawa S, Watarai T, Oyama M, Maruyama H, Araki Y, Hara H, Iinuma M (2012) Quantification of polyphenols and pharamacological analysis of water and ethanol-based extracts of cultivated agarwood leaves. J Nutr Sci Vitaminol 58:136–142. https://doi.org/10.3177/jnsv.58.136
Phan MAT, Wang J, Tang J, Lee YZ, Ng K (2013) Evaluation of α-glucosidase inhibition potential of some flavonoids from Epimedium brevicornum. LWT Food Sci Technol 53:492–498. https://doi.org/10.1016/j.lwt.2013.04.002
Yu Q, Qi J, Yu HX, Chen LL, Kou JP, Liu SJ, Yu BY (2013) Qualitative and quantitative analysis of phenolic compounds in the leaves of Aquilaria sinensis using liquid chromatography-mass spectrometry. Phytochem Anal 24:349–356. https://doi.org/10.1002/pca.2416
Fujita M, Inoue T (1980) Biosynthesis of mangiferin in Anemarrhena asphodeloides bunge. II. C-glucosylation of mangiferin. Chem Pharm Bull 28:2482–2486. https://doi.org/10.1248/cpb.28.2482
Nadilah WA, Ali AM, Mamat WNAW, Mahmod NH (2019) Evaluation of DPPH free radical scavenging, α-glucosidase inhibitory, and antimicrobial activities of Aquilaria malaccensis leaf extracts. J Agrobiotechnology 10:36–45. https://doi.org/10.13140/RG.2.2.25987.35368
Thitikornpong W, Palanuvej C, Ruangrungsi N (2019) In vitro antidiabetic, antioxidation and cytotoxicity activities of ethanolic extract of Aquilaria crassna leaves and its active compound; mangiferin. Indian J Tradit Knowl 18:144–150
Rashid ZM, Nasir NNM, Ahmad WNW, Mahmod NH (2020) α-Glucosidase inhibition, DPPH scavenging activities and chemical analysis of polysaccharide extracts from Aquilaria sp. leaves. J Agrobiotechnology 11:59–69. https://doi.org/10.37231/jab.2020.11.2.225
Acknowledgements
We express our thanks to Mr. Kongwany Sydara, the Traditional Medicine Research Center, Ministry of Health, Lao People’s Democratic Republic, for providing A. rugosa seeds for use in experiments.
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HI and MI were involved in study design and data interpretation. HI performed the data analysis. All authors critically revised the report, commented on drafts of the manuscript, and approved the final report.
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Ito, H., Ito, M. Comparison of phenolic compounds contained in Aquilaria leaves of different species. J Nat Med 76, 693–702 (2022). https://doi.org/10.1007/s11418-022-01608-3
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DOI: https://doi.org/10.1007/s11418-022-01608-3