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Polydiacetylene-based poly-ion complex enabling aggregation-induced emission and photodynamic therapy dual turn-on for on-demand pathogenic bacteria elimination

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Abstract

Poly-ion complex (PIC) integrating non-antibiotic theranostics holds great promise in the combat against drug-resistant bacteria. Photosensitizers with aggregation-induced emission (AIE) characteristic are particularly intriguing theranostic agents, but incorporating them into antibacterial PIC to enable both fluorescence and reactive oxygen species (ROS) generation turn-on deems a great challenge. Here we report the development of a PIC that can dually boost the fluorescence and ROS generation in the presence of pathogen bacteria. The PIC is constructed based on an anionic polydiacetylene poly(deca-4,6-diynedioic acid) (PDDA), which completely degrades in the presence of ROS. A cationic polymer quaternized poly(2-(dimethylamino)ethyl methacrylate) (PQDMA) that can disrupt bacterial membrane is co-loaded together with a highly efficient AIE photosensitizer TPCI in the PIC. PIC is nonfluorescent initially in that PDDA can quench the AIE of TPCI in PIC. When pathogenic bacteria are present, they can disturb the assembly of PIC to release TPCI, the fluorescence of which turns on sensitively to indicate the existence of bacteria. The on-demand irradiation can be subsequently applied to excite TPCI, which generates ROS to degrade PDDA and deform the PIC. As a result, TPCI and PQDMA are completely released to eliminate bacteria through a synergy of turned-on photodynamic therapy (PDT) and membrane disruption. The highly efficient detection and inhibition against both Gram-negative and Gram-positive bacteria have validated this polydiacetylene-based PIC system as an effective non-antibiotic antibacterial theranostic platform as well as a new strategy to enable “turn-on” fluorescence sensing and imaging of AIE fluorophores.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (21877042, 22077038, 22107032), the National Basic Research Plan of China (2018YFA0208903), Postdoctoral Research Foundation of China (2017M622454, 2020T130038ZX), and Huazhong University Startup Fund.

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  1. These authors contributed equally to this work.

Authors and Affiliations

  1. National Engineering Research Center for Nanomedicine, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, China

    Sidan Tian, Yuan Lu, Zhenyan He, Qiang Yue, Zhiyong Zhuang, Fanling Meng & Liang Luo

  2. Key Laboratory of Molecular Biophysics of the Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, China

    Liang Luo

  3. Hubei Key Laboratory of Bioinorganic Chemistry and Materia Medica, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan, 430074, China

    Liang Luo

  4. College of Life Science and Technology, Wuhan Polytechnic University, Wuhan, 430023, China

    Yingzhou Wang

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  1. Sidan Tian
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  2. Yuan Lu
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  3. Zhenyan He
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  4. Qiang Yue
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  8. Liang Luo
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Correspondence to Liang Luo.

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Supporting information The supporting information is available online at http://chem.scichina.com and http://link.springer.com/journal/11426. The supporting materials are published as submitted, without typesetting or editing. The responsibility for scientific accuracy and content remains entirely with the authors.

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11426_2022_1317_MOESM1_ESM.pdf

Polydiacetylene-Based Poly-Ion Complex Enabling Aggregation-Induced Emission and Photodynamic Therapy Dual Turn-on for On-Demand Pathogenic Bacteria Elimination

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Tian, S., Lu, Y., He, Z. et al. Polydiacetylene-based poly-ion complex enabling aggregation-induced emission and photodynamic therapy dual turn-on for on-demand pathogenic bacteria elimination. Sci. China Chem. 65, 1782–1790 (2022). https://doi.org/10.1007/s11426-022-1317-0

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  • DOI: https://doi.org/10.1007/s11426-022-1317-0

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