Abstract
It is essential that certified potatoes are free from known viruses which can negatively affect quality and yield. However, very little is known about the distribution and frequency of Potato virus S (PVS) in Serbia. Until 2014, PVS was present sporadically in the domestic seed potato production. The incidence of PVS was studied by a molecular method over 3 years (2014–2016) in four important potato-growing regions (Moravički, Zlatiborski, Raški and Mačvanski) and in different cultivars. The results showed that the incidence of PVS increased steadily over 3 years from 1.52 to 8.84%. The Moravički region had the highest incidence (13.06%) and Desiree was the most susceptible cultivar with a mean PVS incidence of 8.2% followed by Marabel and Riviera. The highest significant statistical difference was between the cultivars and in the interaction between cultivars and regions. A detailed phylogenic analysis of the tested isolates confirmed that Serbian PVS belongs to PVSO. Of the 18 Serbian PVS isolates included in this study, eight were grouped into the PVSO cluster and formed a subgroup (O-I) with isolates from the USA, Syria, Korea and Chile. Ten Serbian isolates of PVS together with the isolates from Iran were clustered in a branch of subgroup O-VII. This study constitutes the first report of PVS isolates in Serbia which are capable of infecting Chenopodium quinoa and inducing the symptoms of local chlorotic lesions.
Similar content being viewed by others
References
Adams MJ, Antoniw JF (2004) The new plant virus family Flexiviridae and assessment of molecular criteria for species demarcation. Arch Virol 149:1045–1060
Adams MJ, Lefkowitz EJ, King AM, Harrach B, Harrison RL, Knowles NJ, Kropinski AM et al (2016) Ratification vote on taxonomic proposals to the International Committee on Taxonomy of Viruses. Arch Virol 161:2921–2949
Broćić Z, Stefanović R (2012) Potato. University of Belgrade pp 407
Clark MF, Adams AN (1977) Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. J Gen Virol 34:574–586
Cox BA, Jones RAC (2010) Genetic variability in the coat protein gene of Potato virus S isolates and distinguishing its biologically distinct strains. Arch Virol 155:1163–1169
De Bokx JA, Huttinga H (1981) Potato virus Y. Description of plant viruses, No. 242. Commonw MycolInst/AssocApplBiol, Kew, UK
De BruynOboter MP (1952) A new potato virus. In: Proceedings of the Conference on Potato Virus Diseases, Wageningen-Lisse pp 83–84
Dolby CA, Jones RAC (1987) Occurrence of the Andean strain of Potato virus S in imported potato material and its effects on potato cultivars. Plant Pathol 36:381–388
EPPO (1999) Seed potato, certification schemes. Bulletin OEPP/EPPO Bulletin 29:253–267
Faostat (2018) Online Database (available at http://faostat.fao.org/, accessed May 28, 2018)
Fletcher JD (1996) Potato virus S A—characterization of an isolate from New Zealand. ZJ Crop Hortic Sci 24:33–35
Hooker JF (1990) Compendium of potato diseases, 4th edn. American Phytopathological Society, St. Paul 125p
Hutton F, Spink JH, Griffin D, Kildea S, Bonner D, Doherty G, Hunter A (2015) Distribution and incidence of viruses in Irish seed potato crops. Irish J Agric Food Res 54:98–106
Jones RAC (1981) The ecology of viruses infecting wild and cultivated potatoes in the Andean regions of South America. In: Thresh JM (ed) Pests, pathogens and vegetation. Pitman Advanced Pub. Program, London, pp 89–107
Jones RAC, Fribourg CE, Slack SA (1982) Potato virus and virus-like disease. In: Barnett OW, Tollin S (eds) Plant Virus Slide Series, Set No. 2. Clemson University, South Carolina
Lambert SJ, Scott JB, Pethybridge SJ, Hay FS (2012) Strain characterization of Potato virus S isolates from Tasmania, Australia. Plant Dis 96:813–819
Lin YH, Johnson DA, Pappu HR (2014a) Effect of Potato Virus S infection on late blight resistance in potato. Am Potato J 91:642–648
Lin YH, Abad JA, Maroon-Lango CJ, Perry KL, Pappu HR (2014b) Molecular characterization of domestic and exotic potato virus S isolates and a global analysis of genomic sequences. Arch Virol 159:2115–2122
Loebenstein G (2001) Potato leafroll virus. In: Loebenstein G et al (eds) Virus and virus-like diseases of potatoes, and production of seed potatoes. Kluwer Academic Publishers, Dordrecht, pp 69–75
Martelli GP, Adams MJ, Kreuze JF, Dolja VV (2007) Family Flexiviridae: a case study in virion and genome plasticity. Phytopathology 45:73–100
Matoušek J, Schubert J, Dědič P, Ptáček J (2000) A broad variability of potato virus S (PVS) revealed by analysis of virus sequences amplified by reverse transcriptase–polymerase chain reaction. Can J Plant Pathol 22:29–37
Matoušek J, Schubert J, Ptáček J, Kozlová P, Dědič P (2005) Complete nucleotide sequence and molecular probing of potato virus S genome. Acta Virol 49:195–205
Palukaitis P (2012) Resistance to virus of potato and their vectors. Plant Pathol J 28:248–258
Poke FS, Crowle DR, Whittock SP, Wilson CR (2010) Molecular variation of hop mosaic virus isolates. Arch Virol 155:1721–1724
Predajňa I, Šoltys K, Kraic J, Mihălik D (2017) First report of potato virus S infecting tomato in Slovakia. J Plant Pathol 99:811
Ronquist F, Huelsenbeck JP (2003) Mr Bayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19:1572–1574
Rose DG (1983) Some properties of an unusual isolate of potato virus S. Potato Res 26:49–62
Ross H (1986) Potato breeding—problems and perspectives. In: Brandes J, Bartels R, Völk J, Wetter C (eds) Advance in Plant Breeding J Plant Breeding. Paul Parey, Berlin
Salari K, Massumi H, Heydarnejad J, Pour AH, Varsani A (2011) Analysis of Iranian Potato virus S isolates. Virus Genes 43:281–288
Slack S (1981) Identification of an unusual strain of potato virus S in North America. Phytopathology 71:255
Slack SA (1983) Identification of an isolate of the Andean strain of Potato Virus S in North America. Plant Dis 67:786–789
Starović M, Milosavljević A, Pfaf-Dolovac E, Aleksić G, Dolovac N, Kuzmanović S (2015) The incidence of viruses in Serbian potato seed production. In: Marčić D, Glavendekić M, Nicot P (Eds) Proceedings of the 7th Congress on Plant Prot Plant Protection Society of Serbia, IOBC-EPRS, IOBC-WPRS, Belgrade pp 273–276
Stevenson WR, Loria R, Franc GD, Wingartner DP (eds) (2001) Compendium of potato diseases. The American Phytopathological Society, St. Paul, MN
Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Mol Biol Evol 30:2725–2729
Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680
Valkonen J (2007) Viruses: economical losses and biotechnical potential. In: Vreugdenhill D (ed) Potato biology and biotechnology: advances and perspectives. Elsevier Science, Amsterdam, pp 619–641
Vučetić A, Vukov T, Jovičić I, Petrović-Obradović O (2013) Monitoring of aphid flight activities in seed potato crops in Serbia. Zookeys 319:333–346
Acknowledgments
The authors would like to thank Mrs. Svetlana Bajic-Raymond from Bristol, England, for the valuable comments and text corrections
Funding
This work was supported by the Ministry of Education, Science and Technological Development, Republic of Serbia, Project TR31018.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of Interest
The authors declare that they have no conflict of interest.
Rights and permissions
About this article
Cite this article
Ristić, D., Vučurović, I., Kuzmanović, S. et al. The Incidence and Genetic Diversity of Potato virus S in Serbian Seed Potato Crops. Potato Res. 62, 31–46 (2019). https://doi.org/10.1007/s11540-018-9395-y
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11540-018-9395-y