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Magnetic iron oxide nanoparticles mediated gene therapy for breast cancer — An In Vitro study

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Summary

The aim of this study was to evaluate the feasibility and efficacy of using TRAIL gene to treat breast cancer mediated with a novel carrier — magnetic iron oxide nanoparticles (polyMAG-1000) coated with PEI. The magnetic iron oxide nanoparticles were used as gene carrier to transfect TRAIL gene into MCF-7 cells. The polyMAG-1000 without TRAIL gene was transfected into the tumor cells as negative control. TRAIL gene transfection with liposome as carrier served as positive control. The apoptosis of cells was detected with TUNEL method. The apoptosis ratio of tumor cells was measured with flow cytometry (FCM). It was found that the apoptosis occurred in the tumor cells after transfection of TRAIL gene mediated by both polyMAG-1000 and liposome. The apoptosis ratio in the group with polyMAG-1000 as gene carrier was (25.11 ± 2.85) %, whereas it was (5.06 ± 1.05) % in the control group with polyMAG-1000 (P<0.01). The apoptosis ratio was as low as (18.31 ± 2.44) % in the group with liposome as gene carrier (P<0.05, as compared with the group with polyMAG-1000 as gene carrier). It is suggested that TRAIL gene may induce apoptosis in MCF-7 breast cancer cells. The magnetic iron oxide nanoparticles coated with PEI may be a potential gene carrier with high transfection efficacy for cancer gene therapy.

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References

  1. Wang S, El-Deiry W S. TRAIL and apoptosis induction by TNF-family death receptors. Oncogene, 2003,22(53):8628–8633

    Article  PubMed  CAS  Google Scholar 

  2. Seol J Y, Park K H, Hwang C I. Adenovirus-TRAIL can overcome TRAIL resistance and induce a bystander effect. Cancer Gene Ther, 2003,10(7):540–548

    Article  PubMed  CAS  Google Scholar 

  3. Thomas M, Klibanov A M. Non-viral gene therapy: polycation-mediated DNA delivery. Appl Microbiol Biotechnol, 2003,62(1):27–34

    Article  PubMed  CAS  Google Scholar 

  4. LeBlanc H N, Ashkenazi A. Apo2L/TRAIL and its death and decoy receptors. Cell Death Differ, 2003,10(1):66–75

    Article  PubMed  CAS  Google Scholar 

  5. Almasan A, Ashkenazi A. Apo2L/TRAIL: apoptosis signaling, biology, and potential for cancer therapy. Cytokine Growth Factor Rev, 2003,14(3–4):337–348

    Article  PubMed  CAS  Google Scholar 

  6. Lee J, Hampl M, Albert P. Antitumor activity and prolonged expression from a TRAIL-expressing adenoviral vector. Neoplasia, 2002,4(4):312–323

    Article  PubMed  CAS  Google Scholar 

  7. Merdan T, Kopecek J, Kissel T. Prospects for cationic polymers in gene and oligonucleotide therapy against cancer. Adv Drug Deliv Rev, 2002,54(5):715–758

    Article  PubMed  CAS  Google Scholar 

  8. Brokx R, Gariepy J. Peptide-and polymer-based gene delivery vehicles. Methods Mol Med, 2004,90:139–160

    PubMed  CAS  Google Scholar 

  9. Arizono Y, Yoshikawa H, Naganuma H, A mechanism of resistance to TRAIL/Apo2L-induced apoptosis of newly established glioma cell line and sensitisation to TRAIL by genotoxic agents. Br J Cancer, 2003,88(2):298–306.

    Article  PubMed  CAS  Google Scholar 

Download references

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Authors and Affiliations

  1. Wuhan Medical and Health Care center for Woman and Children, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430016, China

    Wei Weizhong M.D., Ph.D.  (韦卫中) (male)

  2. Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China

    Xu Chunfang  (徐春芳)

  3. Department of Nuclear Medicine, The First Hospital of Xiamen, Fujian Medical University, Xiamen, 361003, China

    Wu Hua  (吴华)

Authors
  1. Wei Weizhong M.D., Ph.D.  (韦卫中)
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  2. Xu Chunfang  (徐春芳)
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  3. Wu Hua  (吴华)
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Corresponding author

Correspondence to Wu Hua  (吴华).

Additional information

This project was supported by a grant from the National Natural Science Foundation of China (No. 30171062).

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Wei, W., Xu, C. & Wu, H. Magnetic iron oxide nanoparticles mediated gene therapy for breast cancer — An In Vitro study. J. Huazhong Univ. Sc. Technol. 26, 728–730 (2006). https://doi.org/10.1007/s11596-006-0628-y

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  • DOI: https://doi.org/10.1007/s11596-006-0628-y

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