Abstract
We have cloned a WD-repeat gene from peach. The cloned gene is more than 3 kb and contains signature domains characteristic of WD-repeat genes. Because of its high homology with AtTTG1, we hypothesized that this gene could be a TTG1 ortholog in peach. Functional studies were carried out by complementing the trichome minus Arabidopsis ttg1-1 mutant with the putative peach TTG1 homolog. Successful restoration of normal trichomes was achieved in the resulting transgenics. We further tested the possibility that this gene was the candidate gene differentiating peach and nectarine. Sequence analysis indicated no difference in the full-length TTG1 and 1,600 bp of its promoter between peach and nectarine.
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Acknowledgments
We acknowledge Dr. Alan Lloyd, Texas A&M University, who kindly provided the Arabidopsis ttg1-1 mutant seeds. In addition, we would like to acknowledge the Iranian Ministry of Higher Education (AT), CFI, OIT, OMAFRA, and OTFMB (SJ) for the financial support of this research.
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Taheri, A., Jayasankar, S., Cline, J.A. et al. A WD-repeat gene from peach (Prunus persica L.) is a functional ortholog of Arabidopsis thaliana TRANSPARENT TESTA GLABRA1 . In Vitro Cell.Dev.Biol.-Plant 48, 23–29 (2012). https://doi.org/10.1007/s11627-011-9390-3
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DOI: https://doi.org/10.1007/s11627-011-9390-3