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Screening Azadirachta indica tree for enhancing azadirachtin and oil contents in dry areas of Gujarat, India

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Abstract

Azadirachta indica seed samples were collected from four different agro-ecological regions (AERs) viz., AER-2, AER-4, AER-5A and AER-5B of Gujarat state, India during 2000 to 2002 with an aim to assess variability in azadirachtin, oil and fatty acids content of the seeds and assess correlation of these parameters with morphological characters. Azadirachtin, oil and fatty acids content indicated significant (p<0.01) variations between years and AERs in Gujarat. The azadirachtin ranged from 142 to 9 527 μg·g−1 seed kernel with an average of 2 426 μg·g−1 for the state. AER-5B recorded highest azadirachtin and oil content. Fatty acid composition was found to be affected by environmental factors particularly varying degree of annual rainfall and temperature during fruit ripening period. Conclusively trees growing in AER-5B performed better in term of azadirachtin, oil and stearic acid content. Tree girth at breast height showed no significant relation with these biochemicals.

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References

  • Bally ISE, Ruddle L, Simpson B. 1996. Azadirachtin levels in Neem seed grown in Northern Australia. In: International Neem Conference Feb 4–9, Queensland, Australia, p.17.

  • Demirbas A. 2009. Potential resources of non-edible oils for biodiesel. Energy Sources: Econ Planning Policy, 4: 310–314.

    Article  CAS  Google Scholar 

  • Elteraifi IE, Hassanali A. 2010. Oil and azadirachtin contents of neem (Azadirachta indica A. Juss) seed kernels collected from trees growing in different habitats in sudan. http://wgbis.ces.iisc.ernet.in/energy/lake2006/programme/programme/lake2006_Pdf/Intisar.pdf dated 20th January 2010.

  • Ermel K, Pahlich E, Schmutterer H. 1984. Comparison of the azadirachtin content of neem seeds from ecotypes of Asian and African origin. In: Schnutterer H, Aschier KRS. (eds.) Proceeding of 2nd Int Neem Conf, GTZ, Germany, pp. 91–94.

  • Ermel K, Pahlich E, Schmutterer H. 1986. Azadirachtin content of neem kernels from ecotypes different geographical locations and it dependence on temperature, relative humidity and light. In: Schnutterer H, Aschier KRS. (eds.), Proceeding of 2nd Int Neem Conf, GTZ, Germany, pp. 171–184.

  • Gupta PK, Tripathi YC, Prabhu VV, Pal RS. 1998. Variation in fatty oil and azadirachtin contents of neem seed kernels of different geographical origins. In: Gupta BN, Sharma KK. (eds.), Neem A Wonder Tree. Dehra Dun, India: ICFRE, pp. 142–148.

    Google Scholar 

  • Kaushik N. 2002. Determination of azadirachtin and fatty acid methyl esters of Azadirachta indica seeds by HPLC and GLC. Anal Bioanal Chem, 374: 1199–1204.

    Article  PubMed  CAS  Google Scholar 

  • Kaushik N, Singh BG, Tomar UK, Naik SN, Vir S, Bisla SS, Sharma KK, Banerjee SK, Thakkar P. 2007. Regional and habitat variability in azadirachtin content of Indian neem (Azadirachta indica A. Jusieu). Current Science, 92: 1400–1406.

    Google Scholar 

  • Kumar MG, Kumar RJ, Regupathy A, Rajasekaran B. 1995. Liquid chromatographic determination and monitoring of Azadirachtin in Neem ecotypes. Neem Update, 1: 4–5.

    Google Scholar 

  • Momchilova S, Antonova D, Marekov I, Kuleva L, Damyanova BN, Jham G. 2007. Fatty acids, triacylglycerols, and sterols in Neem oil (Azadirachta Indica A. Juss) as determined by a combination of chromatographic and spectral techniques. J Liquid Chromatography & Related Technology, 30: 11–25.

    Article  CAS  Google Scholar 

  • Muñoz-Valenzuela S, Ibarra-López AA, Rubio-Silva LM, Valdez-Dávila H, Borboa-Flores J. 2007. Neem tree morphology and oil Content. In: Janick, J, hipkey A. (eds.), Issues in new crops and new uses. Alexandria, VA: ASHS Press, pp. 126–128.

    Google Scholar 

  • Pinzi S, Garcia IL, Lopez-Gimenez FJ, Luque de Castro MD, Dorado G, Dorado MP. 2009. The ideal vegetable oil-based biodiesel composition: a review of social, economical and technical implications. Energy Fuels, 23: 2325–2341.

    Article  CAS  Google Scholar 

  • Pradhan S, Jotwani MG, Rai BK 1962. The neem seed deterrent to locusts. Indian Farming, 12: 7–11.

    Google Scholar 

  • Rengasamy S, Kaushik N, Kumar J, Kaul O, Parmar BS. 1993. Azadircahtin content and bioactivity of Neem ecotypes of India. In: Singh RP. (ed.), Proceedings of World Neem Conference. New Delhi: IHCO, pp. 207–211

    Google Scholar 

  • Schmutterer H. 1981. Ten years of Neem research in Federal Republic of Germany. In: Schnutterer H, Aschier KRS, Rembold H. (eds.), Natural pesticide from Neem tree. Proceeding of 1st Int. Neem Conf, GTZ, Germany, pp. 21–32.

  • Schmutterer H, Singh RP. 1995 List of insect pests susceptible to neem products. In: Schnutterer H. (ed.), The Neem Tree. VCH Verlagsgesellsschaft, Weinheim, Federal Republic of Germany, pp. 326–365.

  • Schmutterer H, Zebitz CPW. 1984 Effect of methanolic extracts from seed of single neem trees of Africa and Asian origin on the Epilachnan varivestis & Aedes aegypti. In: Schnutterer H, Aschier KRS. (eds.), Natural pesticide from Neem tree. Proceeding of 2nd Int. Neem Confrence, Rottach-Egern, GTZ, Germany. pp. 83–90.

  • Schroeder N. 1987. A simplified isolation procedure for Azadirachtin. J Natural Products, 50: 241–244.

    Article  CAS  Google Scholar 

  • Sidhu OP, Behl HM. 1996. Seasonal variation in azadirchtins in seeds of Azadirachta indica. Current Science, 70: 1084–1086.

    CAS  Google Scholar 

  • Sidhu OP, Kumar V, Behl HM. 2003. Variability in Neem (Azadirchta indica) with respect to azadirchtin content. J Agri Food Chem, 51: 910–915.

    Article  CAS  Google Scholar 

  • Sridharan S, Venugopal MS, Dhaliwal GS, Arora R, Randhawa NS, Dhawan AK. 1998. Effect of environmental conditions on the yield of azadirachtin and oil in neem. In: Ecological agriculture and sustainable Development: Volume 1, Indian Ecological Society and the Centre for research in Rural and industrial development. Chandigarh, India, pp. 510–518.

    Google Scholar 

  • Zanno PR, Miara E, Nakanaishi K, Elder DL. 1975. Structure of the insect phagorepellant Azadirachtin, application of PRFT/CWD carbon 13 nuclear magnetic resonance. J Am Chem Soc, 97: 1975–1977.

    Article  PubMed  CAS  Google Scholar 

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Correspondence to U. K. Tomar.

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Foundation project: This work is supported by the NOVOD Board, Ministry of Agriculture, Government of India.

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Tomar, U.K., Singh, G. & Kaushik, N. Screening Azadirachta indica tree for enhancing azadirachtin and oil contents in dry areas of Gujarat, India. Journal of Forestry Research 22, 217–224 (2011). https://doi.org/10.1007/s11676-011-0153-0

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