Abstract
Atmospheric pressure chemical ionization (APCI) mass spectrometry (MS) has proven to be a very valuable technique for analysis of lipids from a variety of classes. This instrumental method readily produces useful ions with gentle fragmentation from large neutral molecules such as triacylglycerols and carotenoids, which are often difficult to analyze using other techniques. Molecules that are easily ionized, such as phospholipids, produce molecular ions and diagnostically useful fragment ions that are complementary to those produced by methods such as electrospray ionization MS with collision-induced dissociation. The simplicity and versatility of APCI-MS make it an ideal tool for use in solving hitherto very difficult analytical problems.
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Abbreviations
- ACN:
-
acyl carbon number
- AMVN:
-
azobis(2,4-dimethylvaleronitrile)
- APCI:
-
atmospheric pressure chemical ionization
- API:
-
atmospheric pressure ionization
- Br-MB:
-
3-bromomethyl-7-methoxy-1,4-benzoxazin-2-one
- CID:
-
collision-induced dissociation
- cSFC:
-
capillary supercritical fluid chromatography
- DAG:
-
diacylglycerol
- ECN:
-
equivalent carbon number
- EIC:
-
extracted ion chromatogram
- ELSD:
-
evaporative light-scattering detector
- ESI:
-
electrospray ionization
- FA:
-
fatty acid
- FAME:
-
fatty acid methyl ester
- FI:
-
flow injection
- FID:
-
flame-ionization detection
- GC:
-
gas chromatography
- L:
-
linoleic acid
- LC:
-
liquid chromatography
- Ln:
-
linolenic acid
- LSIMS:
-
liquid secondary ion mass spectrometry
- MAG:
-
monoacylglycerol
- MS:
-
mass spectrometry
- MS/MS:
-
tandem mass spectrometry
- O:
-
oleic acid
- P:
-
palmitic acid
- PC:
-
phosphatidylcholine
- PG:
-
phosphatidylglycerol
- RP-HPLC:
-
reversed-phase high-performance liquid chromatography
- S:
-
stearic acid
- SFC:
-
supercritical fluid chromatography
- SIM:
-
selected ion monitoring
- TAG:
-
triacylglycerol
- THCA:
-
3α,7α,12α-trihydroxy-5β-cholestanic acid
- TSP:
-
thermospray
- UV:
-
ultraviolet
- Vis:
-
visible
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Byrdwell, W.C. Atmospheric pressure chemical ionization mass spectrometry for analysis of lipids. Lipids 36, 327–346 (2001). https://doi.org/10.1007/s11745-001-0725-5
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DOI: https://doi.org/10.1007/s11745-001-0725-5