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Jasmonate-dependent plant defenses mediate soybean thrips and soybean aphid performance on soybean

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Abstract

Insect herbivores from different feeding guilds induce different signaling pathways in plants. In this study, we examined the effects of salicylic acid (SA)- and jasmonic acid (JA)-mediated defenses on performance of insect herbivores from two different feeding guilds: cell-content feeders, soybean thrips and phloem feeders, soybean aphids. We used a combination of RT-qPCR analysis and elicitor-induced plant resistance to determine induction of SA and JA signaling pathways and the impact on herbivore performance. In the early interaction between the host plant and the two herbivores, SA and JA signaling seems to occur simultaneously. But overall, soybean thrips induced JA-related marker genes, whereas soybean aphids increased SA and ABA-related marker genes over a 24-h period. Populations of both soybean thrips and soybean aphids were reduced (47 and 25 %, respectively) in methyl jasmonate (MeJA)-pretreated soybean plants. SA treatment has no effect on either herbivore performance. A combination pretreatment of SA and MeJA did not impact soybean thrips population but reduced soybean aphid numbers which was comparable with MeJA treatment. Our data suggest that SA–JA antagonism could be responsible for the effect of hormone pretreatment on thrips performance, but not on aphid performance. By linking plant defense gene expression and elicitor-induced resistance, we were able to pinpoint the role for JA signaling pathway in resistance to two herbivores from different feeding guilds.

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Acknowledgments

This research was funded by Grants from the Indiana Academy of Sciences and Indiana Soybean Alliance. We thank Jenna Davidson, Lee Danels and Dominic Snowball for their help with the insect performance assays. We would also like to thank two anonymous reviewers for their feedback that significantly improved the manuscript.

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  1. Indiana University-Purdue University Fort Wayne, 2101 E. Coliseum Blvd, Fort Wayne, IN, 46805, USA

    Patrick Selig, Stacy Keough, Vamsi J. Nalam & Punya Nachappa

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  1. Patrick Selig
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  2. Stacy Keough
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  3. Vamsi J. Nalam
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Correspondence to Punya Nachappa.

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Supplementary Fig. 1

Expression of salicylate-responsive PR1 and jasmonate-responsive JAR1 in plants treated with SA or MeJA. Reverse transcriptase-PCR (RT-PCR) was performed on RNA extracted from leaves of soybean plants 24 h after they were treated with 0.1 or 0.5 mM salicylic acid and 0.5 or 1.5 mM MeJA. RNA was also collected from plants that were treated with water as a control (C). Each lane on the gel image represents pooled RNA extracted from leaf tissue collected from three individual plants. Expression of soybean FBOX gene provided the control for RT-PCR (JPEG 27 kb)

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Selig, P., Keough, S., Nalam, V.J. et al. Jasmonate-dependent plant defenses mediate soybean thrips and soybean aphid performance on soybean. Arthropod-Plant Interactions 10, 273–282 (2016). https://doi.org/10.1007/s11829-016-9437-9

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