Skip to main content
SpringerLink
Log in

Production, Purification, and Biochemical Characterization of a Fibrinolytic Enzyme from Thermophilic Streptomyces sp. MCMB-379

  • Published:
Applied Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

Production of the fibrinolytic enzyme was carried out using 2.5-L glass fermentor, culture of thermophilic Streptomyces sp., and glucose yeast extract peptone medium of pH 8.0. Five successive batches were carried out under controlled fermentation conditions viz., agitation 140 rpm, aeration 0.5 vvm, 55 °C, and 18 h. The total protein extracellularly produced in the cell-free broth was ~300–500 mg/L. The enzyme belongs to serine endopeptidase type. Studies on the fibrin degradation indicate that the enzyme degrades the fibrin into small molecular weight products as seen from HPLC profile. Phase-contrast microscopic structure of fibrin showed that enzyme cleaves the fibrin filaments. The ex vivo activity of the actinokinase was compared with 500 IU of urokinase and 350 IU of streptokinase. The ex vivo clot lysis was found to be faster as compared to the commercial available enzymes.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  1. Sumi, H., Hamada, K., Nakanishi, K., & Hiratani, H. (1990). Acta Haematologica, 84, 139–143.

    Article  CAS  Google Scholar 

  2. Collen, D., & Lijen, H. R. (1991). Blood, 75, 3114–3124.

    Google Scholar 

  3. Gesheva, V. (2009). Journal of Microbiology, Biochemistry and Technology, 1(1), 57–58.

    Article  CAS  Google Scholar 

  4. Longstaff, C., Williams, S., & Thelwell, C. (2008). Cardiovascular & Hematological Agents in Medicinal Chemistry, 6(3), 212–223.

    Article  CAS  Google Scholar 

  5. Kim, J. S., Kim, J. E., Choi, B. S., Park, S. E., Sapkota, K., Kim, S., et al. (2008). Mycological Research, 112(Pt 8), 990–998.

    Article  CAS  Google Scholar 

  6. Lee, J., Park, S., Choi, W. A., Lee, K. H., Jeong, Y. K., Kong, I. S., et al. (1999). Journal of Microbiology and Biotechnology, 9(4), 443–449.

    Google Scholar 

  7. Seo, J. H., & Lee, S. P. (2004). Journal of Medicinal Food, 7(4), 442–449.

    Article  CAS  Google Scholar 

  8. Chitte, R. R., & Dey, S. (2000). Letters in Applied Microbiology, 31(6), 405–410.

    Article  CAS  Google Scholar 

  9. Dey, S., & Chitte, R. (2001). Medicinal chemistry Research, 10(78), 507–515.

    CAS  Google Scholar 

  10. Chitte, R., & Dey, S. (2002). World Journal of Microbiology and Biotechnology, 18(4), 289–294.

    Article  CAS  Google Scholar 

  11. Chitte, R., & Dey, S. (2003). US Patent No. 6,638,503.

  12. Dey, S., & Chitte, R. R. (2005). Indian Patent No. 193435.

  13. Hopwood, D. A., Biss, M. J., Chater, K. F., Kieser, T., Bruton, C. J., Ward, J. M., et al. (1985). A laboratory manual. Norwich: The John Inn Foundation.

    Google Scholar 

  14. Lowry, O. H., Rosenberg, W. J., Farr, A. L., & Randell, R. J. (1951). Journal of Biological Chemistry, 193, 265–275.

    CAS  Google Scholar 

  15. Plough, J., & Kjeldgaard, N. (1957). Biochimica et Biophysica Acta, 24, 278–282.

    Article  Google Scholar 

  16. Kim, J. S., Sapkota, K., Park, S. E., Choi, B. S., Kim, S., Hiep, N. T., et al. (2006). A fibrinolytic enzyme from the medicinal mushroom Cordyceps militaris. Journal of Microbiology, 44, 622–631.

    CAS  Google Scholar 

  17. Liu, X., Du, L., Lu, F., Zheng, X., & Xiao, J. (2005). Applied Microbiology and Biotechnology, 67, 209–214.

    Article  CAS  Google Scholar 

  18. Wang, C., Du, M., Zheng, D., Kong, F., & Zu, G. (2009). Journal of Agricultural and Food Chemistry, 57, 9722–9729.

    Article  CAS  Google Scholar 

  19. Veklich, Y. V., Francis, C. W., White, J., & Weisel, J. W. (1998). Blood, 92, 4721–4729.

    CAS  Google Scholar 

  20. Dubey, R., Kumar, J., Agrawala, D., Char, T., & Pusp, P. (2011). African Journal of Biotechnology, 10(8), 1408–1420.

    CAS  Google Scholar 

  21. Peng, Y., Yang, X., & Zhang, Y. (2005). Applied Microbiology and Biotechnology, 69, 126–132.

    Article  CAS  Google Scholar 

  22. Park, S., Li, M. H., Kim, J. S., Sapkota, K., Kim, J. E., Choi, B. S., et al. (2007). Bioscience Biotechnology and Biochemistry, 71(9), 2214–2222.

    Article  CAS  Google Scholar 

  23. Prasad, S., Kashyap, R. S., Deopujari, J. Y., Purohit, H. J., Taori, G. M., & Daginawala, H. F. (2006). Thrombosis Journal, 4(14), 1–4.

    Google Scholar 

Download references

Acknowledgments

The authors are thankful to the Director of ARI for the financial support to this research work.

Author information

Authors and Affiliations

  1. Microbial Sciences Division, MACS-Agharkar Research Institute, G.G. Agarkar Road, Pune, 411004, Maharashtra, India

    Ratnakar Ravindra Chitte, Siddharath V. Deshmukh & Pradnya Pralhad Kanekar

Authors
  1. Ratnakar Ravindra Chitte
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Siddharath V. Deshmukh
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Pradnya Pralhad Kanekar
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Ratnakar Ravindra Chitte.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Chitte, R.R., Deshmukh, S.V. & Kanekar, P.P. Production, Purification, and Biochemical Characterization of a Fibrinolytic Enzyme from Thermophilic Streptomyces sp. MCMB-379. Appl Biochem Biotechnol 165, 1406–1413 (2011). https://doi.org/10.1007/s12010-011-9356-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12010-011-9356-2

Keywords

Search

Navigation