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A Halotolerant Endo-1,4-β-Xylanase from Aspergillus clavatus with Potential Application for Agroindustrial Residues Saccharification

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Abstract

The use of non-potable water (such as seawater) is an attractive alternative for water intensive processes such as biomass pretreatment and saccharification steps in the production of biochemicals and biofuels. Identification and application of halotolerant enzymes compatible with high-salt conditions may reduce the energy needed for non-potable water treatment and decrease waste treatment costs. Here we present the biochemical properties of a halotolerant endo-1,4-β-xylanase produced by Aspergillus clavatus in submerged fermentation, using paper sludge (XPS) and sugarcane bagasse (XSCB), and its potential application in the hydrolysis of agroindustrial residues. The peptide mass fingerprint and amino acid sequencing of the XPS and XSCB enzymes showed primary structure similarities with an endo-1,4-β-xylanase from Aspergillus clavatus (XYNA_ASPCL). Both enzyme preparations presented good thermal stability at 50 °C and were stable over a wide range of pH and Vmax up to 2450 U/mg for XPS. XPS and XSCB were almost fully stable even after 24 h of incubation in the presence of up to 3 M NaCl, and their activity were not affected by 500 mM NaCl. Both enzyme preparations were capable of hydrolyzing paper sludge and sugarcane bagasse to release reducing sugars. These characteristics make this xylanase attractive to be used in the hydrolysis of biomass, particularly with brackish water or seawater.

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Acknowledgments

We thank Fabio M. Squina from Universidade de Sorocaba, Brazil, for providing the A. clavatus NRRL1 strain, Mauricio de Oliveira for technical assistance and Jorge H. A. Betini for providing the paper sludge samples.

Funding

This work was supported by grants from Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)/Brasil-financing Code 001 and Programa de Doutorado Sanduíche no Exterior (PDSE/CAPES no. 88881.186934/2018-01), Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-Process numbers 2010/52322-3, 2014/50884-5 and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, process 563260/2010-6), and the National Institute of Science and Technology of the Bioethanol (465319/2014-9). T.M.P. was recipient of CAPES/PDSE Fellowship and J.C.S.S., A.S.A.S., and R.C.L. were recipients of CAPES Fellowship; T.B.O. is recipient of FAPESP Fellowship (process 2017/09000-4); and J.C.R., R.J.W., M.S.B., and M.L.T.M.P. (process 301963/2017–7) are Research Fellows of CNPq.

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Author notes

  1. Thiago Machado Pasin and José Carlos Santos Salgado contributed equally to this work.

Authors and Affiliations

  1. Department of Biochemistry and Immunology, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, SP, 14049-900, Brazil

    Thiago Machado Pasin, Ana Sílvia de Almeida Scarcella, Rosymar Coutinho de Lucas & Maria de Lourdes Teixeira de Moraes Polizeli

  2. Department of Chemistry and Chemical Biology, Indiana University-Purdue University Indianapolis, Indianapolis, IN, USA

    Thiago Machado Pasin

  3. Department of Chemistry, Faculty of Philosophy, Sciences and Letters of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, 14040-901, Brazil

    José Carlos Santos Salgado & Richard John Ward

  4. Department of Biology, Faculty of Philosophy, Sciences and Letters of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, 14040-901, Brazil

    Tássio Brito de Oliveira, Rosymar Coutinho de Lucas, Mariana Cereia & Maria de Lourdes Teixeira de Moraes Polizeli

  5. Protein Chemistry Center and Department of Molecular and Cell Biology and Pathogenic Bioagents, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, SP, 14049-900, Brazil

    José César Rosa

  6. Institute of Bioscience, University of São Paulo, São Paulo, SP, 05508-090, Brazil

    Marcos Silveira Buckeridge

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  1. Thiago Machado Pasin
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Pasin, T.M., Salgado, J.C.S., Scarcella, A.S.d.A. et al. A Halotolerant Endo-1,4-β-Xylanase from Aspergillus clavatus with Potential Application for Agroindustrial Residues Saccharification. Appl Biochem Biotechnol 191, 1111–1126 (2020). https://doi.org/10.1007/s12010-020-03232-x

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