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Effect of Colloidal Metal Nanoparticles on Biomass, Polysaccharides, Flavonoids, and Melanin Accumulation in Medicinal Mushroom Inonotus obliquus (Ach.:Pers.) Pilát

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Abstract

The article explores effect of colloidal nanoparticles (NPs) of Ag, Fe, and Mg metals on the growth activity of the medicinal mushroom Inonotus obliquus (Ach.:Pers.) Pilát and the synthesis of biologically active compounds (polysaccharides, flavonoids, and melanins). It was found that all the studied NPs stimulated growth activity. AgNPs inhibited polysaccharide and flavonoid synthesis, and stimulated melanin synthesis by 140%. Using MgNPs was effective to increase the level of accumulation of endopolysaccharides, flavonoids, and melanin pigments. FeNPs significantly increased the yield of endopolysaccharides. This effect should be used for biosynthesis stimulation for polysaccharides, flavonoids, and melanins obtaining from I. obliquus cultivated in vitro. The results demonstrate the potential of the use of metal colloidal solutions NPs for the development of environmentally friendly and effective biotechnology to produce biologically active compounds by medicinal macromycete I. obliquus.

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Authors and Affiliations

  1. Institute of Food Biotechnology and Genomics, National Academy of Sciences of Ukraine, Kyiv, 04123, Ukraine

    Natalia Poyedinok

  2. M.G. Kholodny Institute of Botany, National Academy of Sciences of Ukraine, Kyiv, 01601, Ukraine

    Oksana Mykhaylova

  3. Open International University of Human Development “Ukraine”, Kyiv, Ukraine

    Natalia Sergiichuk & Tatyana Tugay

  4. Danilo Zabolotny Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, Kyiv, GSP, 03680, Ukraine

    Andrei Tugay

  5. National University of Life and Environmental Sciences of Ukraine, Kyiv, 03041, Ukraine

    Seraphim Lopatko

  6. Institute of Cell Biology and Genetic Engineering, National Academy of Science of Ukraine, Kyiv, 03143, Ukraine

    Nadiia Matvieieva

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Correspondence to Natalia Poyedinok.

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Poyedinok, N., Mykhaylova, O., Sergiichuk, N. et al. Effect of Colloidal Metal Nanoparticles on Biomass, Polysaccharides, Flavonoids, and Melanin Accumulation in Medicinal Mushroom Inonotus obliquus (Ach.:Pers.) Pilát. Appl Biochem Biotechnol 191, 1315–1325 (2020). https://doi.org/10.1007/s12010-020-03281-2

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