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A Series of Novel Rare Earth Molybdotungstosilicate Heteropolyoxometalates Binding to Bovine Serum Albumin: Spectroscopic Approach

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Abstract

Heteropolyoxometalate complexes have been widely applied in many fields. In this paper, the interaction between a series of novel rare earth molybdotungstosilicate heteropolyoxometalates, K10H3[Ln(SiMo6W5O39)2]·xH2O (abbr. LnW5, Ln = Pr (x = 30), Gd (x = 29), Dy (x = 28), and Yb (x = 31)), and bovine serum albumin (BSA) was investigated by spectroscopic approach under the physiological conditions. In the mechanism discussion, it was proved that the fluorescence quenching of BSA by LnW5 is a result of the formation of LnW5–BSA complex. Fluorescence quenching constants were determined using the Stern–Volmer equation to provide a measure of the binding affinity between LnW5 and BSA. The binding affinity ranked in the order GdW5 > DyW5 > PrW5 > YbW5. The results of thermodynamic parameters ΔG, ΔH, and ΔS at different temperatures indicate that van der Waals interactions and hydrogen bonds play a major role for LnW5–BSA association. Furthermore, the distance r between donor (BSA) and acceptor (LnW5) was obtained according to fluorescence resonance energy transfer.

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Acknowledgments

The authors gratefully acknowledge financial support of the National Natural Science Foundation of China (grant no. 20803019, 20873096, and 20621502), the Research Foundation of Education Bureau of Hubei Province, China (grant no. Q20082205), and Hubei Normal University Foundation, China (grant no. 2007F10).

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Authors and Affiliations

  1. Hubei Key Laboratory of Pollutant Analysis & Reuse Technology, Department of Chemistry, Hubei Normal University, Huangshi, 435002, People’s Republic of China

    Yan-Jun Hu, Yu Ou-Yang & Ai-Min Bai

  2. State Key Laboratory of Virology & Department of Chemistry, Wuhan University, Wuhan, 430072, People’s Republic of China

    Yan-Jun Hu, Ru-Ming Zhao & Yi Liu

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  1. Yan-Jun Hu
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  2. Yu Ou-Yang
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  3. Ai-Min Bai
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  4. Ru-Ming Zhao
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  5. Yi Liu
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Correspondence to Yi Liu.

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Hu, YJ., Ou-Yang, Y., Bai, AM. et al. A Series of Novel Rare Earth Molybdotungstosilicate Heteropolyoxometalates Binding to Bovine Serum Albumin: Spectroscopic Approach. Biol Trace Elem Res 136, 8–17 (2010). https://doi.org/10.1007/s12011-009-8521-8

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