Abstract
Dietary zinc status was recently approved to exert a powerful influence on liver health, and zinc deficiency results in hepatic injury caused by fat deposition, inflammation, and oxidant stress, but the effect of zinc on hepatic lipid metabolism and liver injury in meat duck has not been well defined. To determine the hepatoprotective effects of graded zinc glycine in meat ducks. A total of 384 1-day-old male meat ducks were subjected to 5 weeks feeding program with three experimental diets: (1) low-zinc diet, (2) adequate-zinc diet, and (3) high-zinc diet. Blood and liver samples were collected for biochemical analysis, gene expression analysis, and histopathological study. Diet with low zinc increased hepatic lipid content and triglyceride concentration. Meat ducks fed low-zinc diet exhibited considerably increased serum alanine aminotransferase (ALT) activity than birds fed other diets among all groups (P < 0.05). Low zinc administration also notably induced hepatocyte apoptosis and stimulated hepatic inflammatory gene expression. Adequate or high zinc supplementation increased hepatic zinc level, reduced hepatic lipid deposition and hepatosomatic indices through suppressing the expression of lipogenic genes including fatty acid synthase (FAS) and acetyl-CoA carboxylase (ACC) (P < 0.05), and upregulated the mRNA expression of both fatty acid secretion and β-oxidation, including carnitine palmitoyltransferase 1a (Cpt1a), peroxisome proliferator–activated receptor (PPAR)α, and apolipoprotein B (ApoB) (P < 0.05). Dietary zinc addition also declined hepatic mRNA expression of interleukin (IL)-1β and IL-6 (P < 0.05). Furthermore, diets with adequate or high zinc significantly decreased serum ALT activity and hepatocyte apoptosis. These data revealed that supplementing adequate- or high-zinc glycine efficiently protects liver injury by attenuating lipid deposition and hepatic inflammation.
Similar content being viewed by others
References
Karagozian R, Derdak Z, Baffy G (2014) Obesity-associated mechanisms of hepatocarcinogenesis. Metabolism 63:607–617
Hermier D (1997) Lipoprotein metabolism and fattening in poultry. J Nutr 127:805 s–808 s
Lu L, Chen Y, Wang Z, Li X, Chen W, Tao Z, Shen J, Tian Y, Wang D, Li G, Chen L, Chen F, Fang D, Yu L, Sun Y, Ma Y, Li J, Wang J (2015) The goose genome sequence leads to insights into the evolution of waterfowl and susceptibility to fatty liver. Genome Biol 16:89
Molee W, Bouillier-Oudot M, Auvergne A, Babile R (2005) Changes in lipid composition of hepatocyte plasma membrane induced by overfeeding in duck. Comp Biochem Physiol B Biochem Mol Biol 141:437–444
Hermier D, Guy G, Guillaumin S, Davail S, Andre JM, Hoo-Paris R (2003) Differential channelling of liver lipids in relation to susceptibility to hepatic steatosis in two species of ducks. Comp Biochem Physiol B Biochem Mol Biol 135:663–675
Liu J (2014) Ethanol and liver: recent insights into the mechanisms of ethanol-induced fatty liver. World J Gastroenterol 20:14672–14685
Liu J, Han L, Zhu L, Yu Y (2016) Free fatty acids, not triglycerides, are associated with non-alcoholic liver injury progression in high fat diet induced obese rats. Lipids Health Dis 15:27
Qin S, Han H, Zhang K, Ding X, Bai S, Wang J, Zeng Q (2018) Dietary fibre alleviates hepatic fat deposition via inhibiting lipogenic gene expression in meat ducks. J Anim Physiol Anim Nutr (Berl) 102:e736–e745
Dongiovanni P, Lanti C, Riso P, Valenti L (2016) Nutritional therapy for nonalcoholic fatty liver disease. J Nutr Biochem 29:1–11
Olechnowicz J, Tinkov A, Skalny A, Suliburska J (2018) Zinc status is associated with inflammation, oxidative stress, lipid, and glucose metabolism. J Physiol Sci 68:19–31
Zhou Z, Kang X, Jiang Y, Song Z, Feng W, McClain CJ, Kang YJ (2007) Preservation of hepatocyte nuclear factor-4alpha is associated with zinc protection against TNF-alpha hepatotoxicity in mice. Exp Biol Med (Maywood) 232:622–628
Bao B, Prasad AS, Beck FW, Fitzgerald JT, Snell D, Bao GW, Singh T, Cardozo LJ (2010) Zinc decreases C-reactive protein, lipid peroxidation, and inflammatory cytokines in elderly subjects: a potential implication of zinc as an atheroprotective agent. Am J Clin Nutr 91:1634–1641
Zhong W, Zhao Y, Sun X, Song Z, McClain CJ, Zhou Z (2013) Dietary zinc deficiency exaggerates ethanol-induced liver injury in mice: involvement of intrahepatic and extrahepatic factors. PLoS One 8:e76522
Tupe RS, Tupe SG, Tarwadi KV, Agte VV (2010) Effect of different dietary zinc levels on hepatic antioxidant and micronutrients indices under oxidative stress conditions. Metabolism 59:1603–1611
Huang C, Luo Z, Hogstrand C, Chen F, Shi X, Chen QL, Song YF, Pan YX (2016) Effect and mechanism of waterborne prolonged Zn exposure influencing hepatic lipid metabolism in javelin goby Synechogobius hasta. J Appl Toxicol 36:886–895
Xu C, Huang Z, Liu L, Luo C, Lu G, Li Q, Gao X (2015) Zinc regulates lipid metabolism and MMPs expression in lipid disturbance rabbits. Biol Trace Elem Res 168:411–420
Kang X, Zhong W, Liu J, Song Z, McClain CJ, Kang YJ, Zhou Z (2009) Zinc supplementation reverses alcohol-induced steatosis in mice through reactivating hepatocyte nuclear factor-4alpha and peroxisome proliferator-activated receptor-alpha. Hepatology 50:1241–1250
Zhou Z, Liu J, Song Z, McClain CJ, Kang YJ (2008) Zinc supplementation inhibits hepatic apoptosis in mice subjected to a long-term ethanol exposure. Exp Biol Med (Maywood) 233:540–548
Rishi P, Kaur P, Virdi JS, Shukla G, Koul A (2008) Amelioratory effects of zinc supplementation on Salmonella-induced hepatic damage in the murine model. Dig Dis Sci 53:1063–1070
Kim J, Ahn J (2014) Effect of zinc supplementation on inflammatory markers and adipokines in young obese women. Biol Trace Elem Res 157:101–106
Jarosz Ł, Marek A, Grądzki Z, Kwiecień M, Kalinowski M (2017) The effect of feed supplementation with zinc chelate and zinc sulphate on selected humoral and cell-mediated immune parameters and cytokine concentration in broiler chickens. Res Vet Sci 112:59–65
Jarosz Ł, Marek A, Grądzki Z, Laskowska E, Kwiecień M (2019) Effect of zinc sulfate and zinc glycine chelate on concentrations of acute phase proteins in chicken serum and liver tissue. Biol Trace Elem Res 187:258–272
National Research Council (1994) Nutrient requirements of poultry, 9th edn. National Academy Press, Washington, DC
Kleiner DE, Brunt EM, Van Natta M, Behling C, Contos MJ, Cummings OW, Ferrell LD, Liu YC, Torbenson MS, Unalp-Arida A, Yeh M, McCullough AJ, Sanyal AJ (2005) Design and validation of a histological scoring system for nonalcoholic fatty liver disease. Hepatology 41:1313–1321
Stradiot L, Verhulst S, Roosens T, Oie CI, Moya IM, Halder G, Mannaerts I, van Grunsven LA (2017) Functionality based method for simultaneous isolation of rodent hepatic sinusoidal cells. Biomaterials 139:91–101
Zhou Z, Wang L, Song Z, Saari JT, McClain CJ, Kang YJ (2005) Zinc supplementation prevents alcoholic liver injury in mice through attenuation of oxidative stress. Am J Pathol 166:1681–1690
Elliott WH, Elliott DC (2009) Biochemistry and molecular biology, 4th edn. Oxford University, Oxford, UK
Takai T, Saito Y, Yamamoto K, Tanabe T (1988) Developmental changes of the content of acetyl-CoA carboxylase mRNA in chicken liver. Arch Biochem Biophys 266:313–318
Ribet C, Montastier E, Valle C, Bezaire V, Mazzucotelli A, Mairal A, Viguerie N, Langin D (2010) Peroxisome proliferator-activated receptor-alpha control of lipid and glucose metabolism in human white adipocytes. Endocrinology 151:123–133
Watt AJ, Garrison WD, Duncan SA (2003) HNF4: a central regulator of hepatocyte differentiation and function. Hepatology 37:1249–1253
Kerner J, Hoppel C (2000) Fatty acid import into mitochondria. Biochim Biophys Acta 1486:1–17
Blasiole DA, Davis RA, Attie AD (2007) The physiological and molecular regulation of lipoprotein assembly and secretion. Mol BioSyst 3:608–619
Dowman JK, Tomlinson JW, Newsome PN (2010) Pathogenesis of non-alcoholic fatty liver disease. QJM 103:71–83
Sun Q, Zhong W, Zhang W, Li Q, Sun X, Tan X, Sun X, Dong D, Zhou Z (2015) Zinc deficiency mediates alcohol-induced apoptotic cell death in the liver of rats through activating ER and mitochondrial cell death pathways. Am J Physiol Gastrointest Liver Physiol 308:G757–G766
Jarrar MH, Baranova A, Collantes R, Ranard B, Stepanova M, Bennett C, Fang Y, Elariny H, Goodman Z, Chandhoke V, Younossi ZM (2008) Adipokines and cytokines in non-alcoholic fatty liver disease. Aliment Pharmacol Ther 27:412–421
Goel A, Dani V, Dhawan DK (2005) Protective effects of zinc on lipid peroxidation, antioxidant enzymes and hepatic histoarchitecture in chlorpyrifos-induced toxicity. Chem Biol Interact 156:131–140
Yousef MI, El-Hendy HA, El-Demerdash FM, Elagamy EI (2002) Dietary zinc deficiency induced-changes in the activity of enzymes and the levels of free radicals, lipids and protein electrophoretic behavior in growing rats. Toxicology 175:223–234
Acknowledgments
The authors thank Gregory Fraley, Ahsan Mustafa, and Huaiyong Zhang for the revision of English.
Funding
Financial support was provided by the Science and Technology Support Program of Sichuan Province (Grant No. 2013NZ0054) and the research funding was also provided by Sichuan Longda Animal Husbandry Science and Technology Co., Ltd. (No. 009H2200).
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Animals, diets and experimental design Care, handling and sampling procedures was approved by the Animal Care and Use Committee of Sichuan Agricultural University (approval No. SAU-13-147).
Additional information
Publisher’s Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
About this article
Cite this article
Zhang, Y., Chang, Y., Yang, T. et al. The Hepatoprotective Effects of Zinc Glycine on Liver Injury in Meat Duck Through Alleviating Hepatic Lipid Deposition and Inflammation. Biol Trace Elem Res 195, 569–578 (2020). https://doi.org/10.1007/s12011-019-01860-x
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12011-019-01860-x